Suitable for WB, Dot, EM, IHC, ICC/IF, ELISA, IHC-P. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 47 publications.
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- Toxicological sciences : an official journal of the Society of Toxicology 198:288-3022024Exploring the effects of topoisomerase II inhibitor XK469 on anthracycline cardiotoxicity and DNA damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVeronika Keresteš,Jan Kubeš,Lenka Applová,Petra Kollárová,Olga Lenčová-Popelová,Iuliia Melnikova,Galina Karabanovich,Mushtaq M Khazeem,Hana Bavlovič-Piskáčková,Petra Štěrbová-Kovaříková,Caroline A Austin,Jaroslav Roh,Martin Štěrba,Tomáš Šimůnek,Anna JirkovskáPubMed 38290791
- Acta biochimica et biophysica Sinica 55:866-8772023Monomethyl fumarate prevents alloimmune rejection in mouse heart transplantation by inducing tolerogenic dendritic cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuntao Chen,Yirui Cao,Ouyang Jia,Xuanchuan Wang,Yongsheng Luo,Yin Celeste Cheuk,Tongyu Zhu,Dong Zhu,Yi Zhang,Jina WangPubMed 37184280
- Folia neuropathologica 61:53-672023HSF1 inhibits microglia activation to attenuate neuroinflammation via regulating miR-214-3p and NFATc2 in Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuangao Liao,Yong Gu,Jinhua Wang,Yu Tian,Xiaohong Ni,Lei Zhou,Ye Ye,Guangming XiaPubMed 37114961
- World journal of gastrointestinal oncology 14:2353-23662022Anti-silencing function 1B knockdown suppresses the malignant phenotype of colorectal cancer by inactivating the phosphatidylinositol 3-kinase/AKT pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGen-Hua Yu,Xu-Feng Gong,Ying-Ying Peng,Jun QianPubMed 36568946
- American journal of translational research 14:8129-81452022MiR-216b targets CPEB4 to suppress colorectal cancer progression through inhibiting IL-10-mediated M2 polarization of tumor-associated macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Zou,Boyu Wu,Chao Lin,Qunhua Ding,Jiangtao LiPubMed 36505286
- International journal of molecular sciences 23:2022Deciphering the Role and Signaling Pathways of PKCα in Luminal A Breast Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmilio M Serrano-López,Teresa Coronado-Parra,Consuelo Marín-Vicente,Zoltan Szallasi,Victoria Gómez-Abellán,María José López-Andreo,Marcos Gragera,Juan C Gómez-Fernández,Rubén López-Nicolás,Senena Corbalán-GarcíaPubMed 36430510
- Cell biology international 46:1959-19692022Silencing of COL3A1 represses proliferation, migration, invasion, and immune escape of triple negative breast cancer cells via down-regulating PD-L1 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFan Yang,Ling Lin,Xiaohua Li,Ronglan Wen,Xin ZhangPubMed 35930601
- Experimental & molecular medicine 54:1024-10372022Circular RNA ROCK1, a novel circRNA, suppresses osteosarcoma proliferation and migration via altering the miR-532-5p/PTEN axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYize Liu,Guanzhen Qiu,Yinzhou Luo,Shanshan Li,Yeqiu Xu,Yuanzhuang Zhang,Jiayuan Hu,Peifeng Li,Hai Pan,Yong WangPubMed 35879346
- Molecular medicine (Cambridge, Mass.) 28:292022Gene therapy of yeast NDI1 on mitochondrial complex I dysfunction in rotenone-induced Parkinson's disease models in vitro and vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongzhi Li,Bohao Sun,Yuting Huang,Jing Zhang,Xuejing Xu,Yuqi Shen,Zhuo Chen,Jifeng Yang,Luxi Shen,Yongwu Hu,Haihua GuPubMed 35255803
- Molecular medicine reports 25:2022Comparison of the effect of rotenone and 1‑methyl‑4‑phenyl‑1,2,3,6‑tetrahydropyridine on inducing chronic Parkinson's disease in mouse models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Zhang,Bohao Sun,Jifeng Yang,Zhuo Chen,Zhengzheng Li,Nan Zhang,Hongzhi Li,Luxi ShenPubMed 35039876
Key facts
- Host species
- Goat
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- F(ab')2
- Minimal cross-reactivity
- Mouse, Rat, Sheep, Horse, Cow, Human
- Conjugation
- HRP
- Applications
- WB, Dot, EM, IHC, ICC/IF, ELISA, IHC-P
- Clonality
- Polyclonal
- Format
- F(ab')2
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info 1/2000 - 1/10000 | Notes - |
Application Dot | Reactivity Reacts | Dilution info - | Notes - |
Application EM | Reactivity Reacts | Dilution info - | Notes - |
Application IHC | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/100000 | Notes This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/500 to 1/2000 of the reconstitution concentration is suggested for this product. Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. |
Application IHC-P | Reactivity Reacts | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
Associated Products
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6 products for Alternative Product
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS
Recommended products
Suitable for WB, Dot, EM, IHC, ICC/IF, ELISA, IHC-P. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 47 publications.
Key facts
- Description
- Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed
- Host species
- Goat
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- F(ab')2
- Minimal cross-reactivity
- Mouse, Rat, Sheep, Horse, Cow, Human
- Conjugation
- HRP
- Applications
- WB, Dot, EM, IHC, ICC/IF, ELISA, IHC-P
- Clonality
- Polyclonal
- Format
- F(ab')2
- Pre-adsorbed
- Yes
- Specificity
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Purification technique
- Affinity purification
- Purification notes
Anti-Rabbit IgG F(ab')2 Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed (ab6112)
IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with Anti-beta Actin antibody ab8227, 3μg/ml overnight at +4°C. An HRP-conjugated secondary (ab6112, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Western blot - Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed (ab6112)
ab6112 was used at dilution 1/5000 with the primary antibody Anti-HDAC2 antibody [Y461] ab32117 in WB. See the review on Anti-HDAC2 antibody [Y461] ab32117.
SecondaryAll lanes: Western blot - Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed (ab6112)
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