AB6112

Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed

Name: Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed secondary antibody (ab6112) | Abcam
Brand: Abcam Limited
SKU: AB6112
Price: 295 USD
Availability: InStock

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(57 Publications)

Suitable for WB, Dot, EM, IHC, ICC, ELISA, IHC-P. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 57 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed (AB6112)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed (AB6112)

IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 3μg/ml overnight at +4°C. An HRP-conjugated secondary (ab6112, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

The inset negative control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Unknown

Western blot - Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed (AB6112)

ab6112 was used at dilution 1/5000 with the primary antibody ab32117 in WB. See the review on ab32117.

Secondary

All lanes:

Western blot - Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed (ab6112)

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Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

F(ab')2

Minimal cross-reactivity

Mouse, Rat, Sheep, Horse, Cow, Human

Pre-adsorbed

Yes

Conjugation

HRP

Excitation/Emission

Applications

ELISA, IHC, EM, Dot, ICC, IHC-P, WB

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000 - 1/10000", "notes":"" }, "Dot": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "EM": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/5000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100000", "notes":"This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/500 to 1/2000 of the reconstitution concentration is suggested for this product. Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity." }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500", "notes":" Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Anti-Rabbit IgG F(ab')2 Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Storage buffer

Preservative: 0.01% Gentamicin sulphate Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (57)

Recent publications for all applications. Explore the full list and refine your search

Hereditas 162:176 PubMed40877960

2025

HOXA13 promotes high glucose-induced trophoblast cell growth and migration during gestational diabetes by regulating the smad2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Zhao,Xinhong Liu,Hong Qin

Nature communications 16:4928 PubMed40425539

2025

Topobexin targets the Topoisomerase II ATPase domain for beta isoform-selective inhibition and anthracycline cardioprotection.

Applications

Unspecified application

Species

Unspecified reactive species

Jan Kubeš,Galina Karabanovich,Anh T Q Cong,Iuliia Melnikova,Olga Lenčová,Petra Kollárová,Hana Bavlovič Piskáčková,Veronika Keresteš,Lenka Applová,Lise C M Arrouye,Julia R Alvey,Jasmina Paluncic,Taylor L Witter,Anna Jirkovská,Jiří Kuneš,Petra Štěrbová-Kovaříková,Caroline A Austin,Martin Štěrba,Tomáš Šimůnek,Jaroslav Roh,Matthew J Schellenberg

Frontiers in endocrinology 15:1440654 PubMed39512757

2024

Small phenolic compounds as potential endocrine disruptors interacting with estrogen receptor alpha.

Applications

Unspecified application

Species

Unspecified reactive species

Raul Alva-Gallegos,Eduard Jirkovský,Přemysl Mladěnka,Alejandro Carazo

Molecular medicine reports 30: PubMed39329201

2024

Exosomal miR‑194 from adipose‑derived stem cells impedes hypertrophic scar formation through targeting TGF‑β1.

Applications

Unspecified application

Species

Unspecified reactive species

Zhishan Xu,Yuan Tian,Lijun Hao

World journal of gastrointestinal oncology 16:3241-3255 PubMed39072172

2024

RBM5 suppresses proliferation, metastasis and glycolysis of colorectal cancer cells stabilizing phosphatase and tensin homolog mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Chu-Xiang Wang,Feng Liu,Yi Wang

Cell death & disease 15:456 PubMed38937450

2024

MicroRNA miR-20a-5p targets CYCS to inhibit apoptosis in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Olaniyi Olarewaju,Yuhai Hu,Hsin-Chieh Tsay,Qinggong Yuan,Simon Eimterbäumer,Yu Xie,Renyi Qin,Michael Ott,Amar Deep Sharma,Asha Balakrishnan

World journal of gastroenterology 30:2689-2708 PubMed38855156

2024

Kinesin 26B modulates M2 polarization of macrophage by activating cancer-associated fibroblasts to aggravate gastric cancer occurrence and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Lian-Meng Huang,Ming-Jin Zhang

European journal of histochemistry : EJH 68: PubMed38779782

2024

Spinosin ameliorates osteoarthritis through enhancing the Nrf2/HO-1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Peipei Lu,Shuxiang Li,Caoyang Zhang,Xinyi Jiang,Jinghua Xiang,Hong Xu,Jian Dong,Kun Wang,Yuhua Shi

Functional & integrative genomics 24:38 PubMed38376551

2024

DKC1 aggravates gastric cancer cell migration and invasion through up-regulating the expression of TNFAIP6.

Applications

Unspecified application

Species

Unspecified reactive species

Huihua Chen,Yibo Wu,Yancheng Jiang,Zixuan Chen,Tingjin Zheng

Toxicological sciences : an official journal of the Society of Toxicology 198:288-302 PubMed38290791

2024

Exploring the effects of topoisomerase II inhibitor XK469 on anthracycline cardiotoxicity and DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Veronika Keresteš,Jan Kubeš,Lenka Applová,Petra Kollárová,Olga Lenčová-Popelová,Iuliia Melnikova,Galina Karabanovich,Mushtaq M Khazeem,Hana Bavlovič-Piskáčková,Petra Štěrbová-Kovaříková,Caroline A Austin,Jaroslav Roh,Martin Štěrba,Tomáš Šimůnek,Anna Jirkovská

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed (AB6112)

Western blot - Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed (AB6112)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (57)

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