AB102288

Goat F(ab')2 Anti-Rabbit IgG - (Fab')2, pre-adsorbed

Name: Goat F(ab')2 Anti-Rabbit IgG - (Fab')2, pre-adsorbed secondary antibody (ab102288) | Abcam
Brand: Abcam Limited
SKU: AB102288
Price: 220 USD
Availability: InStock

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(1 Publication)

Suitable for ICC, IHC-P, ELISA, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 1 publication.

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

F(ab')2

Minimal cross-reactivity

Mouse, Rat, Human

Pre-adsorbed

Yes

Applications

IHC-P, ICC, ELISA, WB

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human, mouse and rat IgG was detected. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/2000", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/2000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/30000", "notes":"(Coating: 1/100 - 1/500; Primary: 1/1000 - 1/30000)." }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/30000", "notes":"(Colorimetric: 1/1000 - 1/10000; Chemiluminescent: 1/1000 - 1/30000)." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was solid phase adsorbed to ensure specificity. Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

mBio 13:e0035822 PubMed36102516

2022

Calpain-1: a Novel Antiviral Host Factor Identified in Porcine Small Intestinal Mucus.

Applications

Unspecified application

Species

Unspecified reactive species

Yuchen Li,Xiuyu Wang,En Zhang,Ruiling Liu,Chengjie Yang,Ying Duan,Yuqi Jiang,Qian Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat F(ab')2 Anti-Rabbit IgG - (Fab')2, pre-adsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Applications

Clonality

Isotype

Specificity

Complementary Products

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise