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Suitable for ICC, IHC-P, ELISA, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 1 publication.

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Publications

Key facts

Host species
Goat
Target species
Rabbit
Target isotype
IgG
Target specificity
F(ab')2
Minimal cross-reactivity
Mouse, Rat, Human
Applications
ICC, IHC-P, ELISA, WB
Clonality
Polyclonal
Format
F(ab')2

Reactivity data

Application
ICC
Reactivity
Reacts
Dilution info
1/200.00000 - 1/2000.00000
Notes

-

Application
IHC-P
Reactivity
Reacts
Dilution info
1/200.00000 - 1/2000.00000
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
1/100.00000 - 1/30000.00000
Notes

(Coating: 1/100 - 1/500; Primary: 1/1000 - 1/30000).

Application
WB
Reactivity
Reacts
Dilution info
1/1000.00000 - 1/30000.00000
Notes

(Colorimetric: 1/1000 - 1/10000; Chemiluminescent: 1/1000 - 1/30000).

Alternative names

Recommended products

Suitable for ICC, IHC-P, ELISA, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 1 publication.

Key facts

Description
Goat F(ab')2 Anti-Rabbit IgG - (Fab')2, pre-adsorbed
Host species
Goat
Target species
Rabbit
Target isotype
IgG
Target specificity
F(ab')2
Minimal cross-reactivity
Mouse, Rat, Human
Applications
ICC, IHC-P, ELISA, WB
Clonality
Polyclonal
Format
F(ab')2
Pre-adsorbed
Yes
Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human, mouse and rat IgG was detected. This antibody may cross react with IgG from other species.

Isotype
IgG
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS

Purification technique
Affinity purification Immunogen
Purification notes

Antiserum was solid phase adsorbed to ensure specificity. Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

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