AB6012

Goat F(ab')2 Anti-Rabbit IgG - H&L (Biotin), pre-adsorbed

Name: Goat F(ab')2 Anti Rabbit (IgG) secondary antibody preadsorbed Biotin (ab6012)
Brand: Abcam Limited
SKU: AB6012
Price: 295 USD
Availability: InStock

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(15 Publications)

Suitable for Dot, IM, IHC, IHC-P, WB, ELISA, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 15 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Sheep, Goat, Chicken, Guinea pig, Hamster, Cow, Human, Horse

Pre-adsorbed

Yes

Conjugation

Biotin

Excitation/Emission

Applications

ELISA, IM, Dot, ICC/IF, IHC-P, WB, IHC

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000 - 1/10000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20000 - 1/100000", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/5000", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

EMBO molecular medicine 17:1417-1451 PubMed40374915

2025

Genotype-specific differences in infertile men due to loss-of-function variants in M1AP or ZZS genes.

Applications

Unspecified application

Species

Unspecified reactive species

Nadja Rotte,Jessica E M Dunleavy,Michelle D Runkel,Lina Bosse,Daniela Fietz,Adrian Pilatz,Johanna Kuss,Ann-Kristin Dicke,Sofia B Winge,Sara Di Persio,Christian Ruckert,Verena Nordhoff,Hans-Christian Schuppe,Kristian Almstrup,Sabine Kliesch,Nina Neuhaus,Birgit Stallmeyer,Moira K O'Bryan,Frank Tüttelmann,Corinna Friedrich

Frontiers in endocrinology 15:1483364 PubMed39758349

2025

Umbilical vein remodeling is associated with pregestational maternal overweight.

Applications

Unspecified application

Species

Unspecified reactive species

Kamilla Batista da Silva Souza,Luana Caroline Hochberger,Felippe Egon Castrignano Camargo,Gabriely Santos Silva,Giovanna Castrignano Camargo,João Pedro Lourenço Mello,Fernanda Cristina Alcantara Dos Santos,Fernanda Regina Giachini,Núbia de Souza Lobato,Paula Cristina de Souza Souto

Human reproduction (Oxford, England) 39:892-901 PubMed38365879

2024

Identification of two hidden clinical subgroups among men with idiopathic cryptozoospermia.

Applications

Unspecified application

Species

Unspecified reactive species

Lena Charlotte Schülke,Joachim Wistuba,Verena Nordhoff,Hermann M Behre,Jann-Frederik Cremers,Sabine Kliesch,Sara Di Persio,Nina Neuhaus

Molecular cell 84:1021-1035.e11 PubMed38359823

2024

C19ORF84 connects piRNA and DNA methylation machineries to defend the mammalian germ line.

Applications

Unspecified application

Species

Unspecified reactive species

Ansgar Zoch,Gabriela Konieczny,Tania Auchynnikava,Birgit Stallmeyer,Nadja Rotte,Madeleine Heep,Rebecca V Berrens,Martina Schito,Yuka Kabayama,Theresa Schöpp,Sabine Kliesch,Brendan Houston,Liina Nagirnaja,Moira K O'Bryan,Kenneth I Aston,Donald F Conrad,Juri Rappsilber,Robin C Allshire,Atlanta G Cook,Frank Tüttelmann,Dónal O'Carroll

Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation 17:32-42 PubMed36746123

2023

Testicular Architecture of Men with 46,XX Testicular Disorders of Sex Development.

Applications

Unspecified application

Species

Unspecified reactive species

Mirkka Hiort,Julia Rohayem,Regine Knaf,Sandra Laurentino,Agnethe Berglund,Claus H Gravholt,Jörg Gromoll,Joachim Wistuba

Life science alliance 6: PubMed36446526

2022

Transcriptome analyses in infertile men reveal germ cell-specific expression and splicing patterns.

Applications

Unspecified application

Species

Unspecified reactive species

Lara M Siebert-Kuss,Henrike Krenz,Tobias Tekath,Marius Wöste,Sara Di Persio,Nicole Terwort,Margot J Wyrwoll,Jann-Frederik Cremers,Joachim Wistuba,Martin Dugas,Sabine Kliesch,Stefan Schlatt,Frank Tüttelmann,Jörg Gromoll,Nina Neuhaus,Sandra Laurentino

Cell reports. Medicine 2:100395 PubMed34622232

2021

Single-cell RNA-seq unravels alterations of the human spermatogonial stem cell compartment in patients with impaired spermatogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Di Persio,Tobias Tekath,Lara Marie Siebert-Kuss,Jann-Frederik Cremers,Joachim Wistuba,Xiaolin Li,Gerd Meyer Zu Hörste,Hannes C A Drexler,Margot Julia Wyrwoll,Frank Tüttelmann,Martin Dugas,Sabine Kliesch,Stefan Schlatt,Sandra Laurentino,Nina Neuhaus

Experimental physiology 106:1359-1372 PubMed33605491

2021

Effects of chronic moderate alcohol consumption on right ventricle and pulmonary remodelling.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Filipa Silva,Fábio Sousa-Nunes,Gabriel Faria-Costa,Ilda Rodrigues,João Tiago Guimarães,Adelino Leite-Moreira,Tiago Henriques-Coelho,Rita Negrão,Daniel Moreira-Gonçalves

Andrology 7:827-839 PubMed31250567

2019

Ageing in men with normal spermatogenesis alters spermatogonial dynamics and nuclear morphology in Sertoli cells.

Applications

Unspecified application

Species

Unspecified reactive species

E Pohl,V Höffken,S Schlatt,S Kliesch,J Gromoll,J Wistuba

Oncology letters 10:2812-2820 PubMed26722247

2015

Association between human cytomegalovirus infection and histone acetylation level in various histological types of glioma.

Applications

IHC

Species

Unspecified reactive species

Rui Huang,Dongmeng Qian,Ming Hu,Xue Zhang,Jingyi Song,Ling Li,Hao Chen,Bin Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat F(ab')2 Anti-Rabbit IgG - H&L (Biotin), pre-adsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (15)

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