AB6009

Goat F(ab')2 Anti-Rabbit IgG - H&L (FITC), pre-adsorbed

Name: Goat F(ab')2 Anti Rabbit (IgG) secondary antibody preadsorbed FITC (ab6009)
Brand: Abcam Limited
SKU: AB6009
Price: 295 USD
Availability: InStock

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(8 Publications)

Suitable for WB, Dot, IM, ICC/IF, Flow Cyt, ELISA. Preadsorbed to minimise non-specific binding and high background staining. Cited in 8 publications.

1 Images

Immunohistochemistry (Frozen sections) - Goat F(ab')2 Anti-Rabbit IgG - H&L (FITC), pre-adsorbed (AB6009)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Goat F(ab')2 Anti-Rabbit IgG - H&L (FITC), pre-adsorbed (AB6009)

Sample : mouse embryonic stem cell-differentiated embryoid bodies (EBs)

Preparation :

Fix in 3%PFA in PBS for 30 min at RT
Incubate in 7.5% sucrose-PBS for 3h at RT
Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
Embed the EBs in tissue-Tek OCT compound
Cut frozen sections to 4-20 µm thickness

Primary antibody : Rabbit anti-laminin alpha 1 (basement membrane marker), 1 : 400
Secondary antibody : Goat F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC), pre-adsorbed (ab6009), 1 : 100
Nuclei were counterstained with DAPI
F-actin was stained with rhodamine-phalloidin, 1 : 100

Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Sheep, Goat, Chicken, Guinea pig, Hamster, Cow, Human, Horse

Pre-adsorbed

Yes

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Applications

WB, Flow Cyt, ICC/IF, Dot, IM, ELISA

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/5000", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500 - 1/2500", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/50000", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 97:e0001523 PubMed36794941

2023

Calcium Influx Regulates the Replication of Several Negative-Strand RNA Viruses Including Severe Fever with Thrombocytopenia Syndrome Virus.

Applications

Unspecified application

Species

Unspecified reactive species

Shuzo Urata,Rokusuke Yoshikawa,Jiro Yasuda

Viruses 13: PubMed34068464

2021

Loperamide Inhibits Replication of Severe Fever with Thrombocytopenia Syndrome Virus.

Applications

Unspecified application

Species

Unspecified reactive species

Shuzo Urata,Jiro Yasuda,Masaharu Iwasaki

The Journal of general virology 101:573-586 PubMed32375950

2020

Human BST-2/tetherin inhibits Junin virus release from host cells and its inhibition is partially counteracted by viral nucleoprotein.

Applications

Unspecified application

Species

Unspecified reactive species

Vahid Rajabali Zadeh,Shuzo Urata,Miako Sakaguchi,Jiro Yasuda

Journal of virology 93: PubMed30814285

2019

Species-Specific Pathogenicity of Severe Fever with Thrombocytopenia Syndrome Virus Is Determined by Anti-STAT2 Activity of NSs.

Applications

Unspecified application

Species

Unspecified reactive species

Rokusuke Yoshikawa,Saori Sakabe,Shuzo Urata,Jiro Yasuda

Science immunology 4: PubMed30683669

2019

Impaired enolase 1 glycolytic activity restrains effector functions of tumor-infiltrating CD8 T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lelisa F Gemta,Peter J Siska,Marin E Nelson,Xia Gao,Xiaojing Liu,Jason W Locasale,Hideo Yagita,Craig L Slingluff,Kyle L Hoehn,Jeffrey C Rathmell,Timothy N J Bullock

Biochemical and biophysical research communication 503:631-636 PubMed29906459

2018

The cholesterol, fatty acid and triglyceride synthesis pathways regulated by site 1 protease (S1P) are required for efficient replication of severe fever with thrombocytopenia syndrome virus.

Applications

Unspecified application

Species

Unspecified reactive species

Shuzo Urata,Yukiko Uno,Yohei Kurosaki,Jiro Yasuda

Proceedings of the National Academy of Sciences of 111:12930-5 PubMed25136096

2014

Pseudomonas aeruginosa triggers CFTR-mediated airway surface liquid secretion in swine trachea.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojie Luan,Verónica A Campanucci,Manoj Nair,Orhan Yilmaz,George Belev,Terry E Machen,Dean Chapman,Juan P Ianowski

Blood 106:193-200 PubMed15746078

2005

T-cell generation by lymph node resident progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rafik Terra,Isabelle Louis,Richard Le Blanc,Sophie Ouellet,Juan Carlos Zúñiga-Pflücker,Claude Perreault

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Goat F(ab')2 Anti-Rabbit IgG - H&L (FITC), pre-adsorbed

Immunohistochemistry (Frozen sections) - Goat F(ab')2 Anti-Rabbit IgG - H&L (FITC), pre-adsorbed (AB6009)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Complementary Products

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (8)

Product promise