AB6517

Goat F(ab')2 Anti-Rat IgG - (Fab')2 (HRP), pre-adsorbed

Name: Goat F(ab')2 Anti Rat (IgG (Fab')2) secondary antibody preadsorbed HRP (ab6517)
Brand: Abcam Limited
SKU: AB6517

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(3 Publications)

Suitable for WB, Dot, EM, IHC, ICC, ELISA. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 3 publications.

View Alternative Names

Ig gamma-1 chain C region

Key facts

Host species

Goat

Target species

Rat

Target isotype

IgG

Target specificity

F(ab')2

Minimal cross-reactivity

Horse, Cow, Human

Pre-adsorbed

Yes

Conjugation

HRP

Excitation/Emission

Applications

WB, Dot, ICC, ELISA, IHC, EM

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "EM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "ICC": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/500 - 1/5000", "notes":"" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/10000 - 1/100000", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Storage buffer

Constituents: 1.5% BSA, 1.46% Sodium chloride, 0.164% Sodium phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Development, growth & differentiation 56:640-52 PubMed25330272

2014

Cell-autonomous signal transduction in the Xenopus egg Wnt/β-catenin pathway.

Applications

Species

Unspecified reactive species

Eriko Motomura,Tomohiro Narita,Yuya Nasu,Hirotaka Kato,Ayako Sedohara,Shin-ichiro Nishimatsu,Masao Sakai

Glycobiology 23:677-89 PubMed23363740

2013

Advanced mass spectrometry and chemical analyses reveal the presence of terminal disialyl motif on mouse B-cell glycoproteins.

Applications

Unspecified application

Species

Unspecified reactive species

Shui-Hua Wang,Chih-Ming Tsai,Kuo-I Lin,Kay-Hooi Khoo

PloS one 6:e26508 PubMed22073170

2011

An essential farnesylated kinesin in Trypanosoma brucei.

Applications

Species

Unspecified reactive species

Erin J Engelson,Frederick S Buckner,Wesley C Van Voorhis

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat F(ab')2 Anti-Rat IgG - (Fab')2 (HRP), pre-adsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (3)

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Product promise