Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.
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- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:11529-115452020The P387 thrombospondin-4 variant promotes accumulation of macrophages in atherosclerotic lesions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSantoshi Muppala,Mohammed Tanjimur Rahman,Irene Krukovets,Dmitriy Verbovetskiy,Elzbieta Pluskota,Aaron Fleischman,D Geoffrey Vince,Edward F Plow,Olga Stenina-AdognraviPubMed 32686880
- Cell death & disease 11:532020Effects of thrombospondin-4 on pro-inflammatory phenotype differentiation and apoptosis in macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohammed Tanjimur Rahman et. al.PubMed 31974349
Key facts
- Host species
- Goat
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Fc region
- Minimal cross-reactivity
- Mouse, Human
- Conjugation
- Alexa Fluor® 488
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
- Format
- F(ab')2
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Alternative names
Ig gamma-1 chain C region
Recommended products
Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.
Key facts
- Description
- Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed
- Host species
- Goat
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Fc region
- Minimal cross-reactivity
- Mouse, Human
- Conjugation
- Alexa Fluor® 488
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
- Format
- F(ab')2
- Pre-adsorbed
- Yes
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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18 product images
Immunocytochemistry/ Immunofluorescence - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (ab150161)
ICC/IF image of Anti-Tubulin antibody [YL1/2] - Loading Control ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-Tubulin antibody [YL1/2] - Loading Control ab6160, 2µg/ml) overnight at +4°C. The secondary antibody (green) was ab150161 Alexa Fluor® 488 goat anti-rat IgG (Fc) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Alexa Fluor® - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (ab150161)
Immunocytochemistry/ Immunofluorescence - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (ab150161)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MEF (mouse embryo fibroblast) cells labelling ATG16L1 with Anti-ATG16L1 (phospho S278) antibody [EPR19016] - Rat IgG1 (Chimeric) ab309495 at 1/100 dilution, followed by ab150161 Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).
Confocal image showing increased cytoplasmic staining in MEF cells after treatment with amino acid and serum starvation in HBSS for 3 h.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150161 Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (ab150161)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HL-60 (human acute promyelocytic leukemia promyeloblast) cells labeling Myeloperoxidase with Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) ab300650 at 1/50 dilution (20.26 µg/ml), followed by ab150161 Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 µg/ml) (Green).
Confocal image showing cytoplasmic staining in HL-60 cell line.
Negative control: HeLa (PMID: 12040446).
Anti-beta Tubulin antibody [EPR16774] - Loading Control ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (10µg/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (4 µg/ml) (Red). The nuclear counterstain was DAPI (Blue).
-ve control 1: Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) ab300650 at a 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 at a 1/500 dilution.
-ve control 2: Anti-beta Tubulin antibody [EPR16774] - Loading Control ab179513 at a 1/200 dilution followed by ab150161 at a 1/1000 dilution.
Flow Cytometry - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (ab150161)
Flow cytometric analysis of L-929 (mouse connective tissue fibroblast, Left) / EL4 (mouse lymphoma T lymphocyte, Right) cells labelling PD1 with Anti-PD1 antibody [29F.1A12] ab309360 at 1/1000 dilution (0.1 ug)/Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/5000 dilution was used as the secondary antibody.
Negative control: L-929 (PMID: 1396582). Gated on viable cells.
Flow Cytometry - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (ab150161)
Flow Cytometry analysis of mouse thymocytes (right panel) compared to a rat monoclonal IgG isotype control (left panel).
Anti-CCR9 antibody [9B1] ab303674 used at a 1/1000 dilution.
Secondary is Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) used at a 1/2000 dilution.
Cells were co-stained with APC conjugated anti-CD4. The conjugation was performed by using Anti-CD4 antibody [EPR20122] - BSA and Azide free ab251480 and APC Conjugation Kit - Lightning-Link® (APC Conjugation Kit - Lightning-Link® ab201807).
Gated on viable cells.
Flow Cytometry (Intracellular) - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (ab150161)
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized C6 (rat glial tumor glial cell) cells labeling non-muscle Myosin IIB/MYH10 with Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] - Rat IgG2a (Chimeric) ab300647 at 1/1000 dilution (0.1µg) (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (ab150161)
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling non-muscle Myosin IIB/MYH10 with Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] - Rat IgG2a (Chimeric) ab300647 at 1/1000 dilution (0.1µg) (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (ab150161)
Flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol permeabilized mouse blood cells labeling Myeloperoxidase with Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) ab300650 at 1/1000 dilution (0.1µg)/ Right compared with a rat monoclonal IgG / Left. Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (ab150161)
Flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell, Left) / HL-60 (human acute promyelocytic leukemia promyeloblast, Right) cells labeling Myeloperoxidase with Anti-Myeloperoxidase antibody [EPR20257] - Rat IgG2a (Chimeric) ab300650 at 1/1000 dilution (0.1µg) (Red) compared with a rat monoclonal IgG (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.
Negative control: HeLa (PMID: 12040446).
Flow Cytometry (Intracellular) - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (ab150161)
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labeling non-muscle Myosin IIB/MYH10 with Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] - Rat IgG2a (Chimeric) ab300647 at 1/1000 dilution (0.1µg) (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (ab150161)
Flow cytometric analysis of mouse thymocytes labeling C-C chemokine receptor type 9 with Anti-CCR9 antibody [9B1] ab303674 at 1/1000 dilution (0.1µg) (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
Flow Cytometry (Intracellular) - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (ab150161)
Flow Cytometry analysis of 4% paraformaldehyde-fixed RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling CD68 with Anti-CD68 antibody [FA-11] ab53444 at a 1/100 dilution (1 μg) (Red) compared to Rat monoclonal IgG - Isotype Control (Black) and cells without incubation with primary antibody and secondary antibody (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (ab150161)
Flow Cytometry analysis of WEHI-231 (Mouse B cell lymphoma B lymphocyte, Left) / RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage, Right) cells stained for CD11b using Anti-CD11b antibody [M1/70] ab8878 at a 1/1000 dilution (0.1 μg) (Red) compared to Rat monoclonal IgG - Isotype Control (Black) and cells without incubation with primary antibody and secondary antibody (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.
Negative control: WEHI-231.(PMID: 2457584)
Gated on viable cells.
Flow Cytometry - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (ab150161)
Flow Cytometry analysis of Mouse peripheral blood mononuclear cell (PBMC) cells labelling CD11b with Anti-CD11b antibody [M1/70] ab8878 at a 1/10,000 dilution (0.01 μg) (right) compared to Rat monoclonal IgG - Isotype Control (right). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
Flow Cytometry - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (ab150161)
Flow cytometric analysis of HeLa (human cervical adenocarcinoma epithelial cell, Left) / HepG2 (human hepatocellular carcinoma epithelial cell, Right) cells labelling CEACAM1 + CEACAM3 + CEACAM6 with Anti-CEACAM1 + CEACAM3 + CEACAM6 antibody [YTH71.3] ab104450 at 1/1000 dilution (0.1&micor;g) followed by a secondary antibody Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161)used at a 1/2000 dilution (Red) compared with an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Negative control: HeLa (PMID: 11580753)
Gated on viable cells.
Flow Cytometry - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (ab150161)
Flow Cytometry analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD11b with Anti-CD11b antibody [M1/70] ab8878 at a 1/10,000 dilution (0.01 μg) (right) compared to Rat monoclonal IgG - Isotype Control (right). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
Flow Cytometry (Intracellular) - Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed (ab150161)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SH-SY5Y(human neuroblastoma epithelial cell, Left) / MCF7(human breast adenocarcinoma epithelial cell, Right) cells labelling Cytokeration 19 with Anti-Cytokeratin 19 antibody [EP1580Y] - Rat IgG2a (Chimeric) ab323561 at 1/1000 dilution (0.1 ug) (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/5000 dilution was used as the secondary antibody.
Negative control: SH-SY5Y.
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