AB6257

Goat F(ab')2 Anti-Rat IgG - Fc (HRP), pre-adsorbed

Name: Goat F(ab')2 Anti Rat (IgG Fc) secondary antibody preadsorbed HRP (ab6257)
Brand: Abcam Limited
SKU: AB6257

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(3 Publications)

Suitable for Dot, EM, ICC, IHC, ELISA, WB. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 3 publications.

View Alternative Names

Ig gamma-1 chain C region

Key facts

Host species

Goat

Target species

Rat

Target isotype

IgG

Target specificity

Fc region

Minimal cross-reactivity

Horse, Cow, Human

Pre-adsorbed

Yes

Conjugation

HRP

Excitation/Emission

Applications

IHC, ICC, ELISA, EM, WB, Dot

applications

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthetic Peptide within Rat Ig gamma-1 chain C region. The exact immunogen used to generate this antibody is proprietary information.

P20759

Specificity

No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab) or Bovine, Horse, Human Serum Proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "EM": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500 - 1/2500", "notes":"" }, "IHC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500 - 1/5000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50000 - 1/100000", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/10000", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Storage buffer

Preservative: 0.01% Gentamicin sulphate Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ig gamma-1 chain C region

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 23:166-176 PubMed28620671

2017

An oligodeoxynucleotide with AAAG repeats significantly attenuates burn-induced systemic inflammatory responses via inhibiting interferon regulatory factor 5 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Xiao,Wenting Lu,Xin Li,Peiyan Zhao,Yun Yao,Xiaohong Wang,Ying Wang,Zhipeng Lin,Yongli Yu,Shucheng Hua,Liying Wang

Biomedical papers of the Medical Faculty of the Un 161:252-260 PubMed28452380

2017

Heterogeneity of molecular forms of dipeptidyl peptidase-IV and fibroblast activation protein in human glioblastomas.

Applications

Unspecified application

Species

Unspecified reactive species

Ivana Matrasova,Petr Busek,Eva Balaziova,Aleksi Sedo

Journal of immunology (Baltimore, Md. : 1950) 186:2503-13 PubMed21239719

2011

Proteasome inhibition with bortezomib depletes plasma cells and autoantibodies in experimental autoimmune myasthenia gravis.

Applications

sELISA

Species

Unspecified reactive species

Alejandro M Gomez,Kathleen Vrolix,Pilar Martínez-Martínez,Peter C Molenaar,Marko Phernambucq,Eline van der Esch,Hans Duimel,Fons Verheyen,Reinhard E Voll,Rudolf A Manz,Marc H De Baets,Mario Losen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat F(ab')2 Anti-Rat IgG - Fc (HRP), pre-adsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Immunogen

Specificity

Complementary Products

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (3)

Product promise