AB150162

Goat F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed

Name: Goat F(ab')2 Anti Rat (IgG) secondary antibody preadsorbed Alexa Fluor® 555
Brand: Abcam Limited
SKU: AB150162
Price: 295 USD
Availability: InStock

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(1 Publication)

Suitable for IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 1 publication.

View Alternative Names

Ig gamma-1 chain C region

2 Images

Immunocytochemistry/ Immunofluorescence - Goat F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (AB150162)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Goat F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (AB150162)

ICC/IF image of ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 1μg/ml) overnight at +4°C. The secondary antibody (yellow) was ab150162, Goat F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed, used at 1μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Goat F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (AB150162)

Alexa Fluor®

Unknown

Alexa Fluor® - Goat F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (AB150162)

Key facts

Host species

Goat

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Human

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Applications

ELISA, IHC-Fr, ICC/IF, Flow Cyt, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Reacts with the heavy and light chains of rat IgG, and with the light chains of rat IgM and IgA as demonstrated by ELISA, FLISA, and flow cytometry. Goat F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed shows minimal cross reactivity with mouse immunoglobulins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Use at an assay dependent dilution" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Use at an assay dependent dilution" } } }

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Product details

This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Current biology : CB 31:4713-4726.e4 PubMed34480856

2021

Evolution of crossover interference enables stable autopolyploidy by ensuring pairwise partner connections in Arabidopsis arenosa.

Applications

Unspecified application

Species

Unspecified reactive species

Chris Morgan,Martin A White,F Chris H Franklin,Denise Zickler,Nancy Kleckner,Kirsten Bomblies

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed

Immunocytochemistry/ Immunofluorescence - Goat F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (AB150162)

Alexa Fluor® - Goat F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (AB150162)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

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