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Suitable for IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 1 publication.
Alternative names=Ig gamma-1 chain C region
Goat
Rat
IgG
Heavy & Light chains
Mouse, Human
Alexa Fluor® 555
Ex: 555nm, Em: 565nm
IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA
Polyclonal
F(ab')2
The exact immunogen used to generate this antibody is proprietary information.
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes Use at an assay dependent dilution |
Application ELISA | Reactivity Reacts | Dilution info - | Notes Use at an assay dependent dilution |
Suitable for IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 1 publication.
Alternative names=Ig gamma-1 chain C region
Goat F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed
Goat
Rat
IgG
Heavy & Light chains
Mouse, Human
Alexa Fluor® 555
Ex: 555nm, Em: 565nm
IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA
Polyclonal
F(ab')2
Yes
The exact immunogen used to generate this antibody is proprietary information.
Reacts with the heavy and light chains of rat IgG, and with the light chains of rat IgM and IgA as demonstrated by ELISA, FLISA, and flow cytometry. Goat F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed shows minimal cross reactivity with mouse immunoglobulins.
IgG
Liquid
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Affinity purification Immunogen
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
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