Skip to main content

Suitable for IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 1 publication.

This product has no reviews yet! Submit a review
Host species

Goat

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Human

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Applications

IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA

Clonality

Polyclonal

Format

F(ab')2

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Shipping To:
United States

Reactivity data

Application
IHC-Fr
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ICC/IF
Reactivity
Reacts
Dilution info
1/200.00000 - 1/1000.00000
Notes

-

Application
Flow Cyt
Reactivity
Reacts
Dilution info
1/2000
Notes

-

Application
IHC-P
Reactivity
Reacts
Dilution info
-
Notes
Use at an assay dependent dilution
Application
ELISA
Reactivity
Reacts
Dilution info
-
Notes
Use at an assay dependent dilution

Images

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Suitable for IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 1 publication.

Key facts

Description

Goat F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed

Host species

Goat

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Human

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Applications

IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA

Clonality

Polyclonal

Format

F(ab')2

Pre-adsorbed

Yes

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Reacts with the heavy and light chains of rat IgG, and with the light chains of rat IgM and IgA as demonstrated by ELISA, FLISA, and flow cytometry. Goat F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed shows minimal cross reactivity with mouse immunoglobulins.

Isotype

IgG

Concentration
Loading...

Properties

Form

Liquid

Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com