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Suitable for WB, Dot, EM, IHC, ELISA, ICC/IF. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 5 publications.

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Host species

Goat

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Sheep, Rabbit, Horse, Cow, Human

Conjugation

HRP

Applications

WB, Dot, EM, IHC, ELISA, ICC/IF

Clonality

Polyclonal

Format

F(ab')2

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Reactivity data

Application
WB
Reactivity
Reacts
Dilution info
1/2000 - 1/10000
Notes

-

Application
Dot
Reactivity
Reacts
Dilution info
-
Notes

-

Application
EM
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
1/100000
Notes

-

Application
ICC/IF
Reactivity
Reacts
Dilution info
1/500.00000 - 1/2500.00000
Notes

-

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Suitable for WB, Dot, EM, IHC, ELISA, ICC/IF. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 5 publications.

Key facts

Description

Goat F(ab')2 Anti- Rat IgG - H&L (HRP), pre-adsorbed

Host species

Goat

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Sheep, Rabbit, Horse, Cow, Human

Conjugation

HRP

Applications

WB, Dot, EM, IHC, ELISA, ICC/IF

Clonality

Polyclonal

Format

F(ab')2

Pre-adsorbed

Yes

Specificity

No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.

Isotype

IgG

Concentration
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Properties

Form

Liquid

Storage buffer

pH: 6.5
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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