Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 405) is a secondary antibody with a maximum absorption wavelength of 401nm and a maximum emission wavelength of 421nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 20 publications
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- The Journal of biological chemistry 300:1072632024JNK activity modulates postsynaptic scaffold protein SAP102 and kainate receptor dynamics in dendritic spines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStella-Amrei Kunde,Bettina Schmerl,Judith von Sivers,Elham Ahmadyar,Taanisha Gupta,Nils Rademacher,Hanna L Zieger,Sarah A ShoichetPubMed 38582451
- iScience 26:1083642023Prdm12 represses the expression of the visceral neuron determinants Phox2a/b in developing somatosensory ganglia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSimon Vermeiren,Pauline Cabochette,Maya Dannawi,Simon Desiderio,Alba Sabaté San José,Younes Achouri,Sadia Kricha,Maren Sitte,Gabriela Salinas-Riester,Benoit Vanhollebeke,Jean-François Brunet,Eric J BellefroidPubMed 38025786
- Cells 12:2023Neurofilament Levels in Dendritic Spines Associate with Synaptic Status.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClara-Marie Gürth,Maria Augusta do Rego Barros Fernandes Lima,Victor Macarrón Palacios,Angel Rafael Cereceda Delgado,Jasmine Hubrich,Elisa D'EstePubMed 36980250
- The Journal of neuroscience : the official journal of the Society for Neuroscience 43:2305-23252023A Novel CCK Receptor GPR173 Mediates Potentiation of GABAergic Inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing He,Heng Shi,Ge Zhang,Yujie Peng,Avirup Ghosh,Mengfan Zhang,Xiaofeng Hu,Chunhua Liu,Yue Shao,Shujie Wang,Lijiang Chen,Wenjian Sun,Junfeng Su,Xi Chen,Liang Zhang,Ying-Shing Chan,Duanqing Pei,Micky Tortorella,Yiping Guo,Hong Yan,Jufang HePubMed 36813575
- Journal of neuroinflammation 19:2792022Neuronal nuclear calcium signaling suppression of microglial reactivity is mediated by osteoprotegerin after traumatic brain injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlbrecht Fröhlich,Florian Olde Heuvel,Rida Rehman,Sruthi Sankari Krishnamurthy,Shun Li,Zhenghui Li,David Bayer,Alison Conquest,Anna M Hagenston,Albert Ludolph,Markus Huber-Lang,Tobias Boeckers,Bernd Knöll,Maria Cristina Morganti-Kossmann,Hilmar Bading,Francesco RoselliPubMed 36403069
- Neuron 111:362-371.e62022Dually innervated dendritic spines develop in the absence of excitatory activity and resist plasticity through tonic inhibitory crosstalk.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMason S Kleinjan,William C Buchta,Roberto Ogelman,In-Wook Hwang,Masaaki Kuwajima,Dusten D Hubbard,Dean J Kareemo,Olga Prikhodko,Samantha L Olah,Luis E Gomez Wulschner,Wickliffe C Abraham,Santos J Franco,Kristen M Harris,Won Chan Oh,Matthew J KennedyPubMed 36395772
- The Journal of clinical investigation 132:2022GPR92 activation in islet macrophages controls β cell function in a diet-induced obesity model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCamila O de Souza,Vivian A Paschoal,Xuenan Sun,Lavanya Vishvanath,Qianbin Zhang,Mengle Shao,Toshiharu Onodera,Shiuhwei Chen,Nolwenn Joffin,Lorena Ma Bueno,Rana K Gupta,Da Young OhPubMed 36066975
- PLoS biology 20:e30015032022The synaptic scaffold protein MPP2 interacts with GABAA receptors at the periphery of the postsynaptic density of glutamatergic synapses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBettina Schmerl,Niclas Gimber,Benno Kuropka,Alexander Stumpf,Jakob Rentsch,Stella-Amrei Kunde,Judith von Sivers,Helge Ewers,Dietmar Schmitz,Christian Freund,Jan Schmoranzer,Nils Rademacher,Sarah A ShoichetPubMed 35312684
- Science advances 7:eabh13762021Nanoscale molecular architecture controls calcium diffusion and ER replenishment in dendritic spines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKanishka Basnayake,David Mazaud,Lilia Kushnireva,Alexis Bemelmans,Nathalie Rouach,Eduard Korkotian,David HolcmanPubMed 34524854
- Cell death discovery 7:162021Inhibition of Aurora Kinase B activity disrupts development and differentiation of salivary glands.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbeer K Shaalan et. al.PubMed 33462217
Key facts
- Host species
- Goat
- Target species
- Guinea pig
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 405
- Excitation/Emission
- Ex: 401nm, Em: 421nm
- Applications
- ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
- Clonality
- Polyclonal
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 405) is a secondary antibody with a maximum absorption wavelength of 401nm and a maximum emission wavelength of 421nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 20 publications
Key facts
- Description
- Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 405)
- Host species
- Goat
- Target species
- Guinea pig
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 405
- Excitation/Emission
- Ex: 401nm, Em: 421nm
- Applications
- ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
This antibody reacts with the heavy and light chains of Guinea pig IgG
We recommend storage time at 4°C should be minimal, since this may affect the signal strength.
Fluorochrome chart - a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.
When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies , designed to deliver precise and reliable results.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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2 product images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 405) (ab175678)
ICC/IF image of Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, 1μg/ml) overnight at +4°C. The cells were then incubated with Rabbit Anti-Mouse IgG H&L (HRP) preadsorbed ab7075, rabbit anti-mouse HRP conjugated antibody, for 1h at a 1/250 dilution. The cells were then incubated with ab34580, guinea pig anti-HRP antibody, for 1h at a 1/250 dilution. Lastly, the secondary antibody (blue) was ab175678, Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 405), used at 1μg/ml for 1h, as a quaternary antibody. DRAQ5™ (DRAQ5™ ab108410) was used to stain the cell nuclei (red) at a dilution of 1/4000.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
Alexa Fluor® - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 405) (ab175678)
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