AB175678

Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 405)

Name: Goat Anti-Guinea pig IgG (Alexa Fluor® 405) secondary antibody (ab175678) | Abcam
Brand: Abcam Limited
SKU: AB175678
Price: 215 USD
Availability: InStock

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(35 Publications)

Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 405) is a secondary antibody with a maximum absorption wavelength of 401nm and a maximum emission wavelength of 421nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.

- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 20 publications

2 Images

Immunocytochemistry/ Immunofluorescence - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 405) (AB175678)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 405) (AB175678)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1μg/ml) overnight at +4°C. The cells were then incubated with ab7075, rabbit anti-mouse HRP conjugated antibody, for 1h at a 1/250 dilution. The cells were then incubated with ab34580, guinea pig anti-HRP antibody, for 1h at a 1/250 dilution. Lastly, the secondary antibody (blue) was ab175678, Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 405), used at 1μg/ml for 1h, as a quaternary antibody. DRAQ5™ (ab108410) was used to stain the cell nuclei (red) at a dilution of 1/4000.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 405) (AB175678)

Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 405) (AB175678)

Key facts

Host species

Goat

Target species

Guinea pig

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Alexa Fluor® 405

Excitation/Emission

Ex: 401nm, Em: 421nm

Applications

IHC-P, Flow Cyt, ELISA, IHC-Fr, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) Ex max: Alexa Fluor® 405 = 402nm / DAPI = 359nm Em max: Alexa Fluor® 405 = 421nm / DAPI = 461nm" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" } } }

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Product details

This antibody reacts with the heavy and light chains of Guinea pig IgG

We recommend storage time at 4°C should be minimal, since this may affect the signal strength.

Fluorochrome chart- a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Guinea pig IgG

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Publications (35)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:34613 PubMed41044194

2025

Patch2MAP combines patch-clamp electrophysiology with super-resolution structural and protein imaging in identified single neurons without genetic modification.

Applications

Unspecified application

Species

Unspecified reactive species

Dimitra Vardalaki,Trang L D Pham,Matthew P Frosch,Garth Rees Cosgrove,Bob S Carter,Mark Richardson,Sydney S Cash,Mark T Harnett

iScience 28:111940 PubMed40034120

2025

Mrgprb4-lineage neurons indispensable in pressure induced pleasant sensation are polymodal.

Applications

Unspecified application

Species

Unspecified reactive species

Longhua Du,Hongyi Cheng,Xiang Cui,Qianan Cao,Xia Li,Shuya Wang,Xiaoxi Wang,Yun Liu,Bing Zhu,Xinyan Gao,Kun Liu

Translational psychiatry 14:484 PubMed39632796

2024

Impacts of CACNB4 overexpression on dendritic spine density in both sexes and relevance to schizophrenia.

Applications

Unspecified application

Species

Unspecified reactive species

Emily M Parker,Nathan L Kindja,Rebecca A DeGiosio,Ryan B Salisbury,Josh M Krivinko,Claire E J Cheetham,Matthew L MacDonald,Weijia Fan,Bin Cheng,Robert A Sweet

iScience 27:110535 PubMed39220408

2024

H-Ras induces exuberant dendritic protrusion growth in mature neurons regardless of cell type.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Krüssel,Ishana Deb,Seungkyu Son,Gabrielle Ewall,Minhyeok Chang,Hey-Kyoung Lee,Won Do Heo,Hyung-Bae Kwon

Nature chemical biology 21:109-119 PubMed39090312

2024

Photoproximity labeling of endogenous receptors in the live mouse brain in minutes.

Applications

Unspecified application

Species

Unspecified reactive species

Mikiko Takato,Seiji Sakamoto,Hiroshi Nonaka,Fátima Yuri Tanimura Valor,Tomonori Tamura,Itaru Hamachi

The Journal of biological chemistry 300:107263 PubMed38582451

2024

JNK activity modulates postsynaptic scaffold protein SAP102 and kainate receptor dynamics in dendritic spines.

Applications

Unspecified application

Species

Unspecified reactive species

Stella-Amrei Kunde,Bettina Schmerl,Judith von Sivers,Elham Ahmadyar,Taanisha Gupta,Nils Rademacher,Hanna L Zieger,Sarah A Shoichet

Brain, behavior, and immunity 119:251-260 PubMed38552924

2024

Instability of excitatory synapses in experimental autoimmune encephalomyelitis and the outcome for excitatory circuit inputs to individual cortical neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca L Gillani,Eseza N Kironde,Sara Whiteman,Theodore J Zwang,Brian J Bacskai

Pain 165:1613-1624 PubMed38335004

2024

Bilateral deficiency of Meissner corpuscles and papillary microvessels in patients with acute complex regional pain syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Mehling,Juliane Becker,Jeremy Chen,Sabrina Scriba,Gudrun Kindl,Rafael Jakubietz,Claudia Sommer,Beate Hartmannsberger,Heike L Rittner

eLife 12: PubMed38318851

2024

Depletion of SMN protein in mesenchymal progenitors impairs the development of bone and neuromuscular junction in spinal muscular atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Sang-Hyeon Hann,Seon-Yong Kim,Ye Lynne Kim,Young-Woo Jo,Jong-Seol Kang,Hyerim Park,Se-Young Choi,Young-Yun Kong

iScience 26:108364 PubMed38025786

2023

Prdm12 represses the expression of the visceral neuron determinants Phox2a/b in developing somatosensory ganglia.

Applications

Unspecified application

Species

Unspecified reactive species

Simon Vermeiren,Pauline Cabochette,Maya Dannawi,Simon Desiderio,Alba Sabaté San José,Younes Achouri,Sadia Kricha,Maren Sitte,Gabriela Salinas-Riester,Benoit Vanhollebeke,Jean-François Brunet,Eric J Bellefroid

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 405)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 405) (AB175678)

Alexa Fluor® - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 405) (AB175678)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Complementary Products

Secondary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (35)

Product promise