AB150185

Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 488)

Name: Goat Anti-Guinea pig IgG (Alexa Fluor® 488) secondary antibody (ab150185) | Abcam
Brand: Abcam Limited
SKU: AB150185
Price: 170 USD
Availability: InStock
Rating: 5 (4 reviews)

(4 Reviews)

(80 Publications)

Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 488) is a secondary antibody with a maximum absorption wavelength of 495nm and a maximum emission wavelength of 519nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.

- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 60 publications

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

2 Images

Immunocytochemistry/ Immunofluorescence - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 488) (AB150185)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 488) (AB150185)

PDX1 staining of Rin-5F cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with Triton X-100 for 5 minutes and then blocked with 1% BSA / 0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab47308, Guinea pig polyclonal to PDX1 at 1µg/ml. Cells were then incubated with ab150185 Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 488) at 2µg/ml (shown in pseudocolour Green). Nuclear DNA was labelled in blue with DAPI. The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Images were acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

Alexa Fluor® - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 488) (AB150185)

Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 488) (AB150185)

Key facts

Host species

Goat

Target species

Guinea pig

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Applications

IHC-P, Flow Cyt, ELISA, ICC/IF, IHC-Fr

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"2 µg/mL", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" } } }

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Product details

This antibody reacts with the heavy and light chains of Guinea pig IgG

Fluorochrome chart- a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Guinea pig IgG

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Publications (80)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:691 PubMed41053007

2025

Repeated ablations of mature Tmem10 oligodendrocytes recapitulates key pathological features of multiple sclerosis with prolonged demyelination.

Applications

Unspecified application

Species

Unspecified reactive species

Feiyan Zhu,Haijiao Huang,Yuting Shu,Guoru Ren,Bo Jing,Honglin Tan,Wanxiang Jiang,Yiyuan Cui,Paul F Worley,Bo Xiao,Mina Chen

Nature neuroscience 28:2201-2216 PubMed41044342

2025

Muscle-derived miR-126 regulates TDP-43 axonal local synthesis and NMJ integrity in ALS models.

Applications

Unspecified application

Species

Unspecified reactive species

Ariel Ionescu,Lior Ankol,Anand Ganapathy Subramaniam,Topaz Altman,Iddo Magen,Yahel Cohen,Yehuda Danino,Tal Gradus-Pery,Yoav Niv,Ori Bar Avi,Danielle Geller,Amjd Ibraheem,Ruilei Cheng,Noam Steinberg,Leenor Alfahel,Pauline Duc,Zeynep Ergul-Ulger,Doruk Arslan,Ersin Tan,Florence Rage,Nilo Riva,Angello Quattrini,Can Ebru Bekircan-Kurt,Adrian Israelson,Amir Dori,Eran Hornstein,Eran Perlson

Nature communications 16:4512 PubMed40374608

2025

Sex-specific perturbations of neuronal development caused by mutations in the autism risk gene DDX3X.

Applications

Unspecified application

Species

Unspecified reactive species

Adele Mossa,Lauren Dierdorff,Jeronimo Lukin,Marta Garcia-Forn,Wei Wang,Fatemeh Mamashli,Yeaji Park,Chiara Fiorenzani,Zeynep Akpinar,Janine Kamps,Jörg Tatzelt,Zhuhao Wu,Silvia De Rubeis

PLoS pathogens 21:e1013126 PubMed40294039

2025

Astrocyte-derived complement C3 facilitated microglial phagocytosis of synapses in Staphylococcus aureus-associated neurocognitive deficits.

Applications

Unspecified application

Species

Unspecified reactive species

Haifang Zhang,Qiyuan Jin,Jijie Li,Jiali Wang,Mengqi Li,Qiao Yin,Qi Li,Yuwan Qi,Lingling Feng,Liang Shen,Yuan Qin,Qifei Cong

Glia 73:840-856 PubMed39743758

2025

Effect of Cytoskeletal Linker Protein GAS2L1 on Oligodendrocyte and Myelin Development.

Applications

Unspecified application

Species

Unspecified reactive species

Yanping Zou,Yili Jin,Yuqian Yang,Liuning Zhang,Yuanyu Feng,Yu Long,ZhengTao Xu,Yuehua He,Wei Zheng,Shuming Wang,Yongxiang He,Jiong Li,Huiliang Li,Zhigang Luo,Chun Hu,Lin Xiao

Chinese medicine 19:156 PubMed39529111

2024

Electroacupuncture alleviates neuropathic pain in a rat model of CCD via suppressing P2X3 expression in dorsal root ganglia.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zheng,Minjian Jiang,Zhouyuan Wei,Hengyu Chi,Yurong Kang,Siyi Li,Yinmu Zheng,Xiaofen He,Xiaomei Shao,Jianqiao Fang,Yongliang Jiang

Neurobiology of stress 33:100680 PubMed39502835

2024

Cholecystokinin-expressing interneurons mediated inhibitory transmission and plasticity in basolateral amygdala modulate stress-induced anxiety-like behaviors in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Fang,Xi Chen,Jufang He

Frontiers in molecular neuroscience 17:1417567 PubMed39282658

2024

Dock4 contributes to neuropathic pain by regulating spinal synaptic plasticity in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaochu Fu,Hongyi Li,Zhuanxu Zhu,Wencui Li,Zhihua Ruan,Ruijie Chang,Huixia Wei,Xueqin Xu,Xunliang Xu,Yanqiong Wu

Scientific reports 14:18426 PubMed39117696

2024

High-throughput image processing software for the study of nuclear architecture and gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Adib Keikhosravi,Faisal Almansour,Christopher H Bohrer,Nadezda A Fursova,Krishnendu Guin,Varun Sood,Tom Misteli,Daniel R Larson,Gianluca Pegoraro

Nature communications 15:3330 PubMed38684656

2024

Atypical heat shock transcription factor HSF5 is critical for male meiotic prophase under non-stress conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Saori Yoshimura,Ryuki Shimada,Koji Kikuchi,Soichiro Kawagoe,Hironori Abe,Sakie Iisaka,Sayoko Fujimura,Kei-Ichiro Yasunaga,Shingo Usuki,Naoki Tani,Takashi Ohba,Eiji Kondoh,Tomohide Saio,Kimi Araki,Kei-Ichiro Ishiguro

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 488) (AB150185)

Alexa Fluor® - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 488) (AB150185)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (80)

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Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

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