Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Cited in 11 publications.
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- Addiction neuroscience 5:2023Sex-related differences in endogenous pituitary adenylate cyclase-activating polypeptide (PACAP) in the thalamic paraventricular nucleus: Implications for addiction neuroscience.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGenevieve R Curtis,Andrew T Gargiulo,Brody A Carpenter,Breanne E Pirino,Annie Hawks,Sierra A Coleman,Nawal A Syed,Anuranita Gupta,Jessica R BarsonPubMed 36798694
- Cell reports 36:1097192021Introduction of synaptotagmin 7 promotes facilitation at the climbing fiber to Purkinje cell synapse.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristopher Weyrer,Josef Turecek,Bailey Harrison,Wade G RegehrPubMed 34551307
- Nature communications 12:31842021Meiosis-specific ZFP541 repressor complex promotes developmental progression of meiotic prophase towards completion during mouse spermatogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuki Horisawa-Takada,Chisato Kodera,Kazumasa Takemoto,Akihiko Sakashita,Kenichi Horisawa,Ryo Maeda,Ryuki Shimada,Shingo Usuki,Sayoko Fujimura,Naoki Tani,Kumi Matsuura,Tomohiko Akiyama,Atsushi Suzuki,Hitoshi Niwa,Makoto Tachibana,Takashi Ohba,Hidetaka Katabuchi,Satoshi H Namekawa,Kimi Araki,Kei-Ichiro IshiguroPubMed 34075040
- The Journal of neuroscience : the official journal of the Society for Neuroscience 41:5386-53982021Sleep Loss Drives Brain Region-Specific and Cell Type-Specific Alterations in Ribosome-Associated Transcripts Involved in Synaptic Plasticity and Cellular Timekeeping.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarlos Puentes-Mestril,James Delorme,Lijing Wang,Marcus Donnelly,Donald Popke,Sha Jiang,Sara J AtonPubMed 34001629
- Cell reports 34:1086232021Vesicular Glutamate Transporters (SLCA17 A6, 7, 8) Control Synaptic Phosphate Levels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCyril Cheret,Marcelo Ganzella,Julia Preobraschenski,Reinhard Jahn,Gudrun Ahnert-HilgerPubMed 33440152
- Frontiers in cellular neuroscience 14:6008952020Molecular and Functional Characterization of Neurogenin-2 Induced Human Sensory Neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmy J Hulme et. al.PubMed 33362470
- Cerebral cortex (New York, N.Y. : 1991) 30:2555-25722019Distinct Laminar Requirements for NMDA Receptors in Experience-Dependent Visual Cortical Plasticity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing-Fai Fong,Peter Sb Finnie,Taekeun Kim,Aurore Thomazeau,Eitan S Kaplan,Samuel F Cooke,Mark F BearPubMed 31832634
- Scientific reports 9:160142019SQSTM-1/p62 potentiates HTLV-1 Tax-mediated NF-κB activation through its ubiquitin binding function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAurélien Schwob et. al.PubMed 31690813
- Journal of immunology and regenerative medicine 3:1-122019An elastin-based vasculogenic scaffold promotes marginal islet mass engraftment and function at an extrahepatic site.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSilvia Minardi,Michelle Guo,Xiaomin Zhang,Xunrong LuoPubMed 31681866
- The FEBS journal 287:1176-11942019Identification and classification of epithelial cells in nephron segments by actin cytoskeleton patterns.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGirishkumar Kaitholil Kumaran,Israel HanukogluPubMed 31605441
Key facts
- Host species
- Goat
- Target species
- Guinea pig
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 555
- Excitation/Emission
- Ex: 555nm, Em: 565nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Cited in 11 publications.
Key facts
- Description
- Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 555)
- Host species
- Goat
- Target species
- Guinea pig
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 555
- Excitation/Emission
- Ex: 555nm, Em: 565nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Product promise
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Full details and terms and conditions can be found here:
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3 product images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 555) (ab150186)
ICC/IF image of Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, 1μg/ml) overnight at +4°C. The cells were then incubated with Rabbit Anti-Mouse IgG H&L (HRP) preadsorbed ab7075, rabbit anti-mouse HRP conjugated antibody, for 1h at a 1/250 dilution. The cells were then incubated with ab34580, guinea pig anti-HRP antibody, for 1h at a 1/250 dilution. Lastly, the secondary antibody (yellow) was ab150186, Goat polyclonal Secondary Antibody to Guinea pig IgG - H&L (Alexa Fluor® 555), used at 1μg/ml for 1h, as a quaternary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Immunocytochemistry/ Immunofluorescence - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 555) (ab150186)
HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (yellow) was ab150186, Goat polyclonal Secondary Antibody to Guinea pig IgG - H&L (Alexa Fluor® 555), used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Alexa Fluor® - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 555) (ab150186)
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