Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 555)
5
(1 Review)
|
(17 Publications)
Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Cited in 17 publications.
View Alternative Names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 555) (AB150186)
PDX1 staining of Rin-5F cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with Triton X-100 for 5 minutes and then blocked with 1% BSA / 0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab47308, Guinea pig polyclonal to PDX1 at 1µg/ml. Cells were then incubated with ab150186 Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 555) at 2µg/ml (shown in pseudocolour Magenta). Nuclear DNA was labelled in blue with DAPI. The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
Images were acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
- Alexa Fluor®
Unknown
Alexa Fluor® - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 555) (AB150186)
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Publications (17)
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Nature communications 16:3933 PubMed40287424
2025
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Glia 73:840-856 PubMed39743758
2025
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BMC biology 22:262 PubMed39548509
2024
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Scientific reports 14:11591 PubMed38773220
2024
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Nature communications 15:3330 PubMed38684656
2024
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International journal of nanomedicine 19:2025-2038 PubMed38476283
2024
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Addiction neuroscience 5: PubMed36798694
2023
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Cell reports 36:109719 PubMed34551307
2021
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Nature communications 12:3184 PubMed34075040
2021
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The Journal of neuroscience : the official journal of the Society for Neuroscience 41:5386-5398 PubMed34001629
2021
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