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AB150186

Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 555)

5

(1 Review)

|

(17 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Cited in 17 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

2 Images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 555) (AB150186)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 555) (AB150186)

PDX1 staining of Rin-5F cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with Triton X-100 for 5 minutes and then blocked with 1% BSA / 0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab47308, Guinea pig polyclonal to PDX1 at 1µg/ml. Cells were then incubated with ab150186 Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 555) at 2µg/ml (shown in pseudocolour Magenta). Nuclear DNA was labelled in blue with DAPI. The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Images were acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

Alexa Fluor® - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 555) (AB150186)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 555) (AB150186)

Key facts

Host species

Goat

Target species

Guinea pig

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Applications

IHC-Fr, IHC-P, Flow Cyt, ICC/IF, ELISA

applications

Clonality

Polyclonal

Isotype

IgG

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"2 µg/mL", "notes":"<p></p>" } } }

Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:3933 PubMed40287424

2025

Nitro-oleic acid enhances mitochondrial metabolism and ameliorates heart failure with preserved ejection fraction in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Marion Müller,Torben Schubert,Cornelius Welke,Tibor Maske,Thomas Patschkowski,Elfi Donhauser,Jacqueline Heinen-Weiler,Felix-Levin Hormann,Sven Heiles,Tina Johanna Schulz,Luisa Andrea Lengenfelder,Lucia Landwehrjohann,Elisa Theres Vogt,Bernd Stratmann,Jurek Hense,Simon Lüdtke,Martina Düfer,Elena Tolstik,Johann Dierks,Kristina Lorenz,Tamino Huxohl,Jan-Christian Reil,Vasco Sequeira,Francisco Jose Schopfer,Bruce A Freeman,Volker Rudolph,Uwe Schlomann,Anna Klinke

Glia 73:840-856 PubMed39743758

2025

Effect of Cytoskeletal Linker Protein GAS2L1 on Oligodendrocyte and Myelin Development.

Applications

Unspecified application

Species

Unspecified reactive species

Yanping Zou,Yili Jin,Yuqian Yang,Liuning Zhang,Yuanyu Feng,Yu Long,ZhengTao Xu,Yuehua He,Wei Zheng,Shuming Wang,Yongxiang He,Jiong Li,Huiliang Li,Zhigang Luo,Chun Hu,Lin Xiao

BMC biology 22:262 PubMed39548509

2024

Rotating culture regulates the formation of HepaRG-derived liver organoids via YAP translocation.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoyu Zhong,Lu Zheng,Yi Wu,Shujin Sun,Qing Luo,Guanbin Song,Dongyuan Lü,Mian Long

Scientific reports 14:11591 PubMed38773220

2024

A simple protocol to establish a conditionally immortalized mouse podocyte cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Yujiao Huang,Jie Geng,Mengdan Wang,Wenbin Liu,Haikun Hu,Wei Shi,Mei Li,Guiyang Huo,Guangrui Huang,Anlong Xu

Nature communications 15:3330 PubMed38684656

2024

Atypical heat shock transcription factor HSF5 is critical for male meiotic prophase under non-stress conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Saori Yoshimura,Ryuki Shimada,Koji Kikuchi,Soichiro Kawagoe,Hironori Abe,Sakie Iisaka,Sayoko Fujimura,Kei-Ichiro Yasunaga,Shingo Usuki,Naoki Tani,Takashi Ohba,Eiji Kondoh,Tomohide Saio,Kimi Araki,Kei-Ichiro Ishiguro

International journal of nanomedicine 19:2025-2038 PubMed38476283

2024

Zinc Oxide Nanoparticles Exacerbate Epileptic Seizures by Modulating the TLR4-Autophagy Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Pingyang Ke,Jing Liu,Chengzhi Chen,Sen Luo,Huiwen Gu,Juan Gu,Yan Liu,Yuanlin Ma,Yuan Meng,Liqin Hu,Xin Tian,Fei Xiao

Addiction neuroscience 5: PubMed36798694

2023

Sex-related differences in endogenous pituitary adenylate cyclase-activating polypeptide (PACAP) in the thalamic paraventricular nucleus: Implications for addiction neuroscience.

Applications

Unspecified application

Species

Unspecified reactive species

Genevieve R Curtis,Andrew T Gargiulo,Brody A Carpenter,Breanne E Pirino,Annie Hawks,Sierra A Coleman,Nawal A Syed,Anuranita Gupta,Jessica R Barson

Cell reports 36:109719 PubMed34551307

2021

Introduction of synaptotagmin 7 promotes facilitation at the climbing fiber to Purkinje cell synapse.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher Weyrer,Josef Turecek,Bailey Harrison,Wade G Regehr

Nature communications 12:3184 PubMed34075040

2021

Meiosis-specific ZFP541 repressor complex promotes developmental progression of meiotic prophase towards completion during mouse spermatogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuki Horisawa-Takada,Chisato Kodera,Kazumasa Takemoto,Akihiko Sakashita,Kenichi Horisawa,Ryo Maeda,Ryuki Shimada,Shingo Usuki,Sayoko Fujimura,Naoki Tani,Kumi Matsuura,Tomohiko Akiyama,Atsushi Suzuki,Hitoshi Niwa,Makoto Tachibana,Takashi Ohba,Hidetaka Katabuchi,Satoshi H Namekawa,Kimi Araki,Kei-Ichiro Ishiguro

The Journal of neuroscience : the official journal of the Society for Neuroscience 41:5386-5398 PubMed34001629

2021

Sleep Loss Drives Brain Region-Specific and Cell Type-Specific Alterations in Ribosome-Associated Transcripts Involved in Synaptic Plasticity and Cellular Timekeeping.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos Puentes-Mestril,James Delorme,Lijing Wang,Marcus Donnelly,Donald Popke,Sha Jiang,Sara J Aton
View all publications

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