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AB150188

Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 594)

1

(1 Review)

|

(20 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Cited in 20 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

2 Images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 594) (AB150188)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 594) (AB150188)

PDX1 staining of Rin-5F cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with Triton X-100 for 5 minutes and then blocked with 1% BSA / 0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab47308, Guinea pig polyclonal to PDX1 at 1 µg/ml. Cells were then incubated with ab150188 Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 594) at 2 µg/ml (shown in pseudocolour Magenta). Nuclear DNA was labelled in blue with DAPI. The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Images were acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

Alexa Fluor® - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 594) (AB150188)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 594) (AB150188)

Key facts

Host species

Goat

Target species

Guinea pig

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Applications

IHC-P, ELISA, Flow Cyt, IHC-Fr, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"2 µg/mL", "notes":"<p></p>" } } }

Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Neuroscience bulletin : PubMed40835804

2025

The Bed Nucleus of the Stria Terminalis-Paraventricular Nucleus of the Hypothalamus Neural Circuit Regulates Neuropathic Pain Through the Brain-Spleen Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shoumeng Han,Xin Chen,Li Ma,Xin Zeng,Ying Wang,Tingting Xie,Fancan Wu,Kun Song,Kenji Hashimoto,Hanbing Wang,Long Wang

Biomolecules 14: PubMed39456189

2024

Cellular Profile of Subfornical Organ Insulin Receptors in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Han-Rae Kim,Jin-Kwon Jeong,Colin N Young

ACS applied materials & interfaces 16:45956-45968 PubMed39182190

2024

Carbonate Apatite Honeycomb Scaffold-Based Drug Delivery System for Repairing Osteoporotic Bone Defects.

Applications

Unspecified application

Species

Unspecified reactive species

Koichiro Hayashi,Cheng Zhang,Ahmad Nazir Taleb Alashkar,Kunio Ishikawa

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2304940 PubMed38417114

2024

Beta-Cell Tipe1 Orchestrates Insulin Secretion and Cell Proliferation by Promoting Gαs/cAMP Signaling via USP5.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Ding,Yang Sun,Yan Liang,Jie Zhang,Zhendong Fu,Caiyue Ren,Pengfei Li,Wen Liu,Rong Xiao,Hao Wang,Zhaoying Zhang,Xuetian Yue,Chunyang Li,Zhuanchang Wu,Yuemin Feng,Xiaohong Liang,Chunhong Ma,Lifen Gao

Cells 13: PubMed38247840

2024

Is Involved in Congenital Chylothorax.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan B Whitchurch,Sophia Schneider,Alina C Hilger,Ricarda Köllges,Jil D Stegmann,Lea Waffenschmidt,Laura Dyer,Holger Thiele,Bhanupriya Dhabhai,Tikam Chand Dakal,Andreas Müller,Dominic P Norris,Heiko M Reutter

iScience 26:106871 PubMed37275527

2023

Zhx2 maintains islet β-cell mass and function by transcriptionally regulating Pax6.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Ding,Yankun Zhang,Yingchun Wang,Yuzhen Wang,Zheng Tong,Pengfei Li,Chaojia Chen,Bo Wang,Xuetian Yue,Chunyang Li,Zhuanchang Wu,Xiaohong Liang,Chunhong Ma,Lifen Gao

Molecular genetics and metabolism reports 35:100964 PubMed36967721

2023

BALB.NCT- is a unique mouse model of hereditary coproporphyria.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojing Kang,Shin Shimada,Hiroki Miyahara,Keiichi Higuchi,Masayuki Mori

Cell transplantation 32:9636897221149130 PubMed36635947

2023

VEGF-Expressing Mesenchymal Stem Cell Therapy for Safe and Effective Treatment of Pain in Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Man Li,Ji Li,Hong Chen,Mingxin Zhu

The Journal of biological chemistry 298:102585 PubMed36223838

2022

Tick-borne encephalitis virus capsid protein induces translational shutoff as revealed by its structural-biological analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Selinger,Radim Novotný,Jakub Sýs,Justin A Roby,Hana Tykalová,Ganji Sri Ranjani,Marie Vancová,Kateřina Jaklová,Filip Kaufman,Marshall E Bloom,Zbyněk Zdráhal,Libor Grubhoffer,Jade K Forwood,Richard Hrabal,Michaela Rumlová,Ján Štěrba

Computational and structural biotechnology journal 20:2759-2777 PubMed35685361

2022

Integrative RNA profiling of TBEV-infected neurons and astrocytes reveals potential pathogenic effectors.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Selinger,Pavlína Věchtová,Hana Tykalová,Petra Ošlejšková,Michaela Rumlová,Ján Štěrba,Libor Grubhoffer
View all publications

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