Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Cited in 47 publications.
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- Theranostics 14:363-3782024The plasticity of neuropeptide Y-Y1 receptor system on neurons contributes to mechanical hyperknesis during chronic itch.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDanqing Dai,Tiantian Zhao,Zhen Li,Wanrong Li,Aiwen Chen,Yali Tang,Xiao-Fei Gao,Lize XiongPubMed 38164144
- Cell death & disease 14:5702023Knockdown of Porf-2 restores visual function after optic nerve crush injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDi Chen,Yi-Yu Sun,Lai-Yang Zhou,Xu Han,Shuo Yang,Fei-Yang Hong,Yuan Yuan,Xiao-Hua Wu,Guo-Hui Huang,Yuan-Chi Cheng,Ju Huang,Dong-Fu FengPubMed 37640747
- Communications biology 6:8462023Sexual dimorphism in the social behaviour of Cntnap2-null mice correlates with disrupted synaptic connectivity and increased microglial activity in the anterior cingulate cortex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatt S Dawson,Kevin Gordon-Fleet,Lingxin Yan,Vera Tardos,Huanying He,Kwong Mui,Smriti Nawani,Zeinab Asgarian,Marco Catani,Cathy Fernandes,Uwe DrescherPubMed 37582968
- BMC musculoskeletal disorders 24:5852023Expression of proinflammatory cytokines and proinsulin by bone marrow-derived cells for fracture healing in long-term diabetic mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHitomi Fujikawa,Hideto Kojima,Tomoya Terashima,Miwako Katagi,Takafumi Yayama,Kosuke Kumagai,Kanji Mori,Hideki Saito,Shinji ImaiPubMed 37464323
- Nature communications 14:33332023Spatial distribution of three ARGONAUTEs regulates the anther phasiRNA pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHinako Tamotsu,Koji Koizumi,Alejandro Villar Briones,Reina KomiyaPubMed 37286636
- Nature aging 3:34-462023Nucleophagy delays aging and preserves germline immortality.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMargarita-Elena Papandreou,Georgios Konstantinidis,Nektarios TavernarakisPubMed 37118512
- Glia 71:1592-16062023Disrupting cortical astrocyte Ca signaling in developing brain induces social deficits and depressive-like behaviors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRong Luo,Bin Zhou,Ping Liao,Yunxia Zuo,Ruotian JiangPubMed 36932889
- The European journal of neuroscience 57:940-9502023Ketamine and its metabolite 2R,6R-hydroxynorketamine promote ocular dominance plasticity and release tropomyosin-related kinase B from inhibitory control without reducing perineuronal nets enwrapping parvalbumin interneurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCecilia Cannarozzo,Anna Rubiolo,Plinio Casarotto,Eero CastrénPubMed 36740723
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e22061012023Mimicking the Biological Sense of Taste In Vitro Using a Taste Organoids-on-a-Chip System.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianguo Wu,Changming Chen,Chunlian Qin,Yihong Li,Nan Jiang,Qunchen Yuan,Yan Duan,Mengxue Liu,Xinwei Wei,Yiqun Yu,Liujing Zhuang,Ping WangPubMed 36638268
- eNeuro 10:2023Automated Image Analysis Reveals Different Localization of Synaptic Gephyrin C4 Splice Variants.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFilip Liebsch,Fynn R Eggersmann,Yvonne Merkler,Peter Kloppenburg,Günter SchwarzPubMed 36543537
Key facts
- Host species
- Goat
- Target species
- Guinea pig
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Applications
- ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
- Clonality
- Polyclonal
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 2 µg/mL | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Cited in 47 publications.
Key facts
- Description
- Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 647)
- Host species
- Goat
- Target species
- Guinea pig
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Applications
- ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Specificity
This antibody may cross react with IgG from other species.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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2 product images
Alexa Fluor® - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 647) (ab150187)
Immunocytochemistry/ Immunofluorescence - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 647) (ab150187)
PDX1 staining of Rin-5F cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with Triton X-100 for 5 minutes and then blocked with 1% BSA / 0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with Anti-PDX1 antibody ab47308, Guinea pig polyclonal to PDX1 at 1µg/ml. Cells were then incubated with ab150187 Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 647) at 2µg/ml (shown in pseudocolour Magenta). Nuclear DNA was labelled in blue with DAPI. The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
Images were acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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