AB150187

Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 647)

Name: Goat Anti-Guinea Pig IgG (Alexa Fluor® 647) secondary antibody (ab150187) | Abcam
Brand: Abcam Limited
SKU: AB150187
Price: 165 USD
Availability: InStock

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(62 Publications)

Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 647) is a secondary antibody with a maximum absorption wavelength of 650nm and a maximum emission wavelength of 665nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.

- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 40 publications

2 Images

Immunocytochemistry/ Immunofluorescence - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 647) (AB150187)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 647) (AB150187)

PDX1 staining of Rin-5F cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with Triton X-100 for 5 minutes and then blocked with 1% BSA / 0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab47308, Guinea pig polyclonal to PDX1 at 1µg/ml. Cells were then incubated with ab150187 Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 647) at 2µg/ml (shown in pseudocolour Magenta). Nuclear DNA was labelled in blue with DAPI. The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Images were acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

Alexa Fluor® - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 647) (AB150187)

Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 647) (AB150187)

Key facts

Host species

Goat

Target species

Guinea pig

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Applications

ICC/IF, IHC-Fr, Flow Cyt, ELISA, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"2 µg/mL", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" } } }

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Product details

This antibody reacts with the heavy and light chains of Guinea pig IgG

Fluorochrome chart- a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Guinea pig IgG

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Publications (62)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8293 PubMed40957893

2025

Gephyrin filaments represent the molecular basis of inhibitory postsynaptic densities.

Applications

Unspecified application

Species

Unspecified reactive species

Arthur Macha,Filip Liebsch,Emanuel H W Bruckisch,Nele Burdina,Imke von Stülpnagel,Konrad Benting,Monika Gunkel,Elmar Behrmann,Guenter Schwarz

Molecular neurodegeneration advances 1:3 PubMed40838110

2025

Dose-ranging and further therapeutic evaluation of a bicistronic humanized TrkB-BDNF gene therapy for glaucoma in rodents.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew Osborne,Tasneem Z Khatib,Michael Whitehead,Terrance Mensah,Sadat Yazdouni,Bart Nieuwenhuis,Zara Ali,Jared Ching,Robert Watt,Naoki Kishi,Naoki Kozono,James R Tribble,Peter S Widdowson,Keith R Martin

Scientific reports 15:29738 PubMed40804138

2025

Functional discrimination of CSF from Alzheimer's patients in a brain on chip platform.

Applications

Unspecified application

Species

Unspecified reactive species

Louise Miny,Jessica Rontard,Ahmad Allouche,Nicolas Violle,Louise Dubuisson,Aurélie Batut,Alexandre Ponomarenko,Rania Talbi,Hélène Gautier,Benoît G C Maisonneuve,Serge Roux,Florian Larramendy,Thibault Honegger,Isabelle Quadrio

Journal of neurochemistry 169:e70169 PubMed40781785

2025

Phosphoinositide- and Collybistin-Dependent Synaptic Clustering of Gephyrin.

Applications

Unspecified application

Species

Unspecified reactive species

Nele Burdina,Filip Liebsch,Arthur Macha,Joaquín Lucas Ortuño Gil,Pia Frommelt,Irina Rais,Fabian Basler,Simon Pöpsel,Guenter Schwarz

PloS one 20:e0313556 PubMed39908332

2025

Transient ocular hypertension remodels astrocytes through S100B.

Applications

Unspecified application

Species

Unspecified reactive species

Weiran Huang,Kenji Matsushita,Rumi Kawashima,Susumu Hara,Yuichi Yasukura,Kaito Yamaguchi,Shinichi Usui,Koichi Baba,Andrew J Quantock,Kohji Nishida

Theranostics 15:1376-1398 PubMed39816678

2025

Heat acclimation defense against exertional heat stroke by improving the function of preoptic TRPV1 neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Li,Ziqing Zhou,You Wu,Jianshuai Zhao,Haokai Duan,Yuliang Peng,Xiaoke Wang,Zhongmin Fan,Lu Yin,Mengyun Li,Fuhong Liu,Yongheng Yang,Lixia Du,Jin Li,Haixing Zhong,Wugang Hou,Fanglin Zhang,Hongwei Ma,Xijing Zhang

Neurobiology of stress 33:100680 PubMed39502835

2024

Cholecystokinin-expressing interneurons mediated inhibitory transmission and plasticity in basolateral amygdala modulate stress-induced anxiety-like behaviors in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Fang,Xi Chen,Jufang He

Journal of radiation research 65:640-650 PubMed39238338

2024

Luminal progenitor and mature cells are more susceptible than basal cells to radiation-induced DNA double-strand breaks in rat mammary tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Kento Nagata,Mayumi Nishimura,Kazuhiro Daino,Yukiko Nishimura,Yuya Hattori,Ritsuko Watanabe,Daisuke Iizuka,Akinari Yokoya,Keiji Suzuki,Shizuko Kakinuma,Tatsuhiko Imaoka

PloS one 19:e0306423 PubMed39088455

2024

Excitatory to inhibitory synaptic ratios are unchanged at presymptomatic stages in multiple models of ALS.

Applications

Unspecified application

Species

Unspecified reactive species

Calum Bonthron,Sarah Burley,Matthew J Broadhead,Vanya Metodieva,Seth G N Grant,Siddharthan Chandran,Gareth B Miles

Nature communications 15:5805 PubMed38987240

2024

Histamine H receptors in dentate gyrus-projecting cholinergic neurons of the medial septum suppress contextual fear retrieval in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Li Cheng,Ling Xiao,Wenkai Lin,Minzhu Li,Jiaying Liu,Xiaoyun Qiu,Menghan Li,Yanrong Zheng,Cenglin Xu,Yi Wang,Zhong Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 647)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 647) (AB150187)

Alexa Fluor® - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 647) (AB150187)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Complementary Products

Secondary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (62)

Product promise