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AB175758

Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 750)

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(3 Publications)

Suitable for WB, ICC/IF, ELISA, IHC-P, Flow Cyt, IHC-Fr. Cited in 3 publications.
2 Images
Western blot - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 750) (AB175758)
  • WB

Lab

Western blot - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 750) (AB175758)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab6046 overnight at 4°C. Antibody binding was detected using ab175758 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-beta Tubulin antibody - Loading Control (<a href='/en-us/products/primary-antibodies/beta-tubulin-antibody-loading-control-ab6046'>ab6046</a>) at 1 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 750) (ab175758) at 1/10000 dilution

Predicted band size: 36 kDa

Observed band size: 50 kDa

false

Alexa Fluor® - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 750) (AB175758)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 750) (AB175758)

Key facts

Host species

Goat

Target species

Guinea pig

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 750

Excitation/Emission

Ex: 749nm, Em: 775nm

Applications

WB, Flow Cyt, ELISA, IHC-Fr, IHC-P, ICC/IF

applications

Clonality

Polyclonal

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p>This applicaiton is no longer batch tested</p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>This applicaiton is no longer batch tested</p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>This applicaiton is no longer batch tested</p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p>This applicaiton is no longer batch tested</p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>This applicaiton is no longer batch tested</p>" } } }

Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature 646:1243-1253 PubMed40931078

2025

Functional synapses between neurons and small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Vignesh Sakthivelu,Anna Schmitt,Franka Odenthal,Kristiano Ndoci,Marian Touet,Ali H Shaib,Abdulla Chihab,Gulzar A Wani,Pascal Nieper,Griffin G Hartmann,Isabel Pintelon,Ilmars Kisis,Maike Boecker,Naja M Eckert,Manoela Iannicelli Caiaffa,Olta Ibruli,Julia Weber,Roman Maresch,Christina M Bebber,Ali Chitsaz,Anna Lütz,Mira Kim Alves Carpinteiro,Kaylee M Morris,Camilla A Franchino,Jonas Benz,Laura Pérez-Revuelta,Jorge A Soriano-Campos,Maxim A Huetzen,Jonas Goergens,Milica Jevtic,Hannah M Jahn-Kelleter,Hans Zempel,Aleksandra Placzek,Alexandru A Hennrich,Karl-Klaus Conzelmann,Hannah L Tumbrink,Pascal Hunold,Joerg Isensee,Lisa Werr,Felix Gaedke,Astrid Schauss,Marielle Minère,Marie Müller,Henning Fenselau,Yin Liu,Alena Heimsoeth,Gülce S Gülcüler Balta,Henning Walczak,Christian Frezza,Ron D Jachimowicz,Julie George,Marcel Schmiel,Johannes Brägelmann,Tim Hucho,Silvia von Karstedt,Martin Peifer,Alessandro Annibaldi,Robert Hänsel-Hertsch,Thorsten Persigehl,Holger Grüll,Martin L Sos,Guido Reifenberger,Matthias Fischer,Dirk Adriaensen,Reinhard Büttner,Julien Sage,Inge Brouns,Roland Rad,Roman K Thomas,Max Anstötz,Silvio O Rizzoli,Matteo Bergami,Elisa Motori,Hans Christian Reinhardt,Filippo Beleggia

Journal of chemical neuroanatomy 131:102284 PubMed37164181

2023

Characterization of three cholinergic inputs to the cochlear nucleus.

Applications

Unspecified application

Species

Unspecified reactive species

Nichole L Beebe,Yoani N Herrera,William A Noftz,Michael T Roberts,Brett R Schofield

Journal of the American Society of Nephrology : JASN 32:1097-1112 PubMed33789950

2021

The Rhesus Macaque Serves As a Model for Human Lateral Branch Nephrogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Meredith P Schuh,Lyan Alkhudairy,Andrew Potter,S Steven Potter,Kashish Chetal,Kairavee Thakkar,Nathan Salomonis,Raphael Kopan
View all publications

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