Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 750)
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(3 Publications)
- WB
Lab
Western blot - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 750) (AB175758)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab6046 overnight at 4°C. Antibody binding was detected using ab175758 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes:
Western blot - Anti-beta Tubulin antibody - Loading Control (<a href='/en-us/products/primary-antibodies/beta-tubulin-antibody-loading-control-ab6046'>ab6046</a>) at 1 µg/mL
All lanes:
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 750) (ab175758) at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 50 kDa
false
- Alexa Fluor®
Unknown
Alexa Fluor® - Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 750) (AB175758)
Reactivity data
Product details
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Shipped at conditions
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Publications (3)
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Nature 646:1243-1253 PubMed40931078
2025
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Journal of chemical neuroanatomy 131:102284 PubMed37164181
2023
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Journal of the American Society of Nephrology : JASN 32:1097-1112 PubMed33789950
2021
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Unspecified reactive species
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