AB7140

Goat Anti-Guinea pig IgG H&L (Alkaline Phosphatase) preadsorbed

Name: Goat Anti Guinea pig (IgG) secondary antibody preadsorbed AP (ab7140) | Abcam
Brand: Abcam Limited
SKU: AB7140
Price: 295 USD
Availability: InStock

Be the first to review this product! Submit a review

(4 Publications)

Suitable for WB, Dot, ELISA, IHC-Fr, IHC-P, IM, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 4 publications.

1 Images

Supplier Data

Dot Blot - Goat Anti-Guinea pig IgG H&L (Alkaline Phosphatase) preadsorbed (AB7140)

Dot blot analysis of ab7140. Detected using Alkaline Phosphatase Substrate (ab7468) for 30 seconds at RT.

All lanes:

Dot Blot - Goat Anti-Guinea pig IgG H&L (Alkaline Phosphatase) preadsorbed (ab7140)

Lane 1:

Guinea Pig IgG at 200 ng

Lane 2:

Guinea Pig IgG at 66.67 ng

Lane 3:

Guinea Pig IgG at 22.2 ng

Lane 4:

Guinea Pig IgG at 7.4 ng

Lane 5:

Guinea Pig IgG at 2.47 ng

Secondary

All lanes:

Goat anti-Guinea Pig IgG Antibody (Min X 10) Alkaline Phosphatase Conjugated Antibody at 1/1000 dilution

false

Key facts

Host species

Goat

Target species

Guinea pig

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Sheep, Rabbit, Goat, Horse, Chicken, Hamster, Cow, Human

Pre-adsorbed

Yes

Conjugation

Alkaline Phosphatase

Excitation/Emission

Applications

IM, WB, Dot, ELISA, ICC/IF, IHC-P, IHC-Fr

applications

Clonality

Polyclonal

Specificity

No reaction was observed against Bovine, Chicken, Goat, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "Dot": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/200 - 1/1000", "notes":"" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage buffer

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.788% Tris HCl, 0.00952% Magnesium chloride, 0.00136% Zinc chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information Guinea pig IgG

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Genes 12: PubMed34356103

2021

Heterozygous Mutation Affects Glucose Tolerance in Male Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Lucie Šedová,Jan Prochazka,Dagmar Zudová,Běla Bendlová,Josef Včelák,Radislav Sedlacek,Ondřej Šeda

Science immunology 5: PubMed32527802

2020

The receptor binding domain of the viral spike protein is an immunodominant and highly specific target of antibodies in SARS-CoV-2 patients.

Applications

Unspecified application

Species

Unspecified reactive species

Lakshmanane Premkumar,Bruno Segovia-Chumbez,Ramesh Jadi,David R Martinez,Rajendra Raut,Alena Markmann,Caleb Cornaby,Luther Bartelt,Susan Weiss,Yara Park,Caitlin E Edwards,Eric Weimer,Erin M Scherer,Nadine Rouphael,Srilatha Edupuganti,Daniela Weiskopf,Longping V Tse,Yixuan J Hou,David Margolis,Alessandro Sette,Matthew H Collins,John Schmitz,Ralph S Baric,Aravinda M de Silva

medRxiv : the preprint server for health sciences : PubMed32511572

2020

The RBD Of The Spike Protein Of SARS-Group Coronaviruses Is A Highly Specific Target Of SARS-CoV-2 Antibodies But Not Other Pathogenic Human and Animal Coronavirus Antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Lakshmanane Premkumar,Bruno Segovia-Chumbez,Ramesh Jadi,David R Martinez,Rajendra Raut,Alena Markmann,Caleb Cornaby,Luther Bartelt,Susan Weiss,Yara Park,Caitlin E Edwards,Eric Weimer,Erin M Scherer,Nadine Roupael,Sri Edupuganti,Daniela Weiskopf,Longping V Tse,Yixuan J Hou,David Margolis,Alessandro Sette,Matthew H Collins,John Schmitz,Ralph S Baric,Aravinda M de Silva

Scientific reports 8:704 PubMed29335496

2018

Localization and function of neurosecretory protein GM, a novel small secretory protein, in the chicken hypothalamus.

Applications

Unspecified application

Species

Unspecified reactive species

Kenshiro Shikano,Yuki Bessho,Masaki Kato,Eiko Iwakoshi-Ukena,Shusuke Taniuchi,Megumi Furumitsu,Tetsuya Tachibana,George E Bentley,Lance J Kriegsfeld,Kazuyoshi Ukena

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat Anti-Guinea pig IgG H&L (Alkaline Phosphatase) preadsorbed

Dot Blot - Goat Anti-Guinea pig IgG H&L (Alkaline Phosphatase) preadsorbed (AB7140)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Specificity

Complementary Products

Reagents, Controls & Accessories

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

Product promise