Suitable for WB, Dot, ELISA, IHC-Fr, IHC-P, IM, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 5 publications.
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Publications
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- Genes 12:2021Heterozygous Mutation Affects Glucose Tolerance in Male Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLucie Šedová,Jan Prochazka,Dagmar Zudová,Běla Bendlová,Josef Včelák,Radislav Sedlacek,Ondřej ŠedaPubMed 34356103
- Science immunology 5:2020The receptor binding domain of the viral spike protein is an immunodominant and highly specific target of antibodies in SARS-CoV-2 patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLakshmanane Premkumar,Bruno Segovia-Chumbez,Ramesh Jadi,David R Martinez,Rajendra Raut,Alena Markmann,Caleb Cornaby,Luther Bartelt,Susan Weiss,Yara Park,Caitlin E Edwards,Eric Weimer,Erin M Scherer,Nadine Rouphael,Srilatha Edupuganti,Daniela Weiskopf,Longping V Tse,Yixuan J Hou,David Margolis,Alessandro Sette,Matthew H Collins,John Schmitz,Ralph S Baric,Aravinda M de SilvaPubMed 32527802
- medRxiv : the preprint server for health sciences :2020The RBD Of The Spike Protein Of SARS-Group Coronaviruses Is A Highly Specific Target Of SARS-CoV-2 Antibodies But Not Other Pathogenic Human and Animal Coronavirus Antibodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLakshmanane Premkumar,Bruno Segovia-Chumbez,Ramesh Jadi,David R Martinez,Rajendra Raut,Alena Markmann,Caleb Cornaby,Luther Bartelt,Susan Weiss,Yara Park,Caitlin E Edwards,Eric Weimer,Erin M Scherer,Nadine Roupael,Sri Edupuganti,Daniela Weiskopf,Longping V Tse,Yixuan J Hou,David Margolis,Alessandro Sette,Matthew H Collins,John Schmitz,Ralph S Baric,Aravinda M de SilvaPubMed 32511572
- Scientific reports 8:7042018Localization and function of neurosecretory protein GM, a novel small secretory protein, in the chicken hypothalamus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKenshiro Shikano et. al.PubMed 29335496
Key facts
- Host species
- Goat
- Target species
- Guinea pig
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Sheep, Rabbit, Goat, Horse, Chicken, Hamster, Cow, Human
- Conjugation
- Alkaline Phosphatase
- Applications
- WB, Dot, ELISA, IHC-Fr, IHC-P, IM, ICC/IF
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Expected | Dilution info - | Notes - |
Application Dot | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Expected | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Expected | Dilution info - | Notes - |
Application IHC-P | Reactivity Expected | Dilution info - | Notes - |
Application IM | Reactivity Expected | Dilution info - | Notes - |
Application ICC/IF | Reactivity Expected | Dilution info 1/200 - 1/1000 | Notes - |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Suitable for WB, Dot, ELISA, IHC-Fr, IHC-P, IM, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 5 publications.
Key facts
- Description
- Goat Anti-Guinea pig IgG H&L (Alkaline Phosphatase) preadsorbed
- Host species
- Goat
- Target species
- Guinea pig
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Sheep, Rabbit, Goat, Horse, Chicken, Hamster, Cow, Human
- Conjugation
- Alkaline Phosphatase
- Applications
- WB, Dot, ELISA, IHC-Fr, IHC-P, IM, ICC/IF
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Specificity
No reaction was observed against Bovine, Chicken, Goat, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins.
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 8
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.788% Tris HCl, 0.00952% Magnesium chloride, 0.00136% Zinc chloride- Purification technique
- Affinity purification
- Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Dot Blot - Goat Anti-Guinea pig IgG H&L (Alkaline Phosphatase) preadsorbed (ab7140)
Dot blot analysis of ab7140. Detected using Alkaline Phosphatase Substrate (NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen ab7468) for 30 seconds at RT.
All lanes: Dot Blot - Goat Anti-Guinea pig IgG H&L (Alkaline Phosphatase) preadsorbed (ab7140)
Lane 1: Guinea Pig IgG at 200 ng
Lane 2: Guinea Pig IgG at 66.67 ng
Lane 3: Guinea Pig IgG at 22.2 ng
Lane 4: Guinea Pig IgG at 7.4 ng
Lane 5: Guinea Pig IgG at 2.47 ng
SecondaryAll lanes: Goat anti-Guinea Pig IgG Antibody (Min X 10) Alkaline Phosphatase Conjugated Antibody at 1/1000 dilution
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