Suitable for Flow Cyt, IHC-Fr, IHC-P, IM, ELISA, ICC/IF, Dot. Preadsorbed to minimise non-specific binding and high background staining.
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application Flow Cyt | Reactivity Expected | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Expected | Dilution info - | Notes - |
Application IHC-P | Reactivity Expected | Dilution info - | Notes - |
Application IM | Reactivity Expected | Dilution info - | Notes - |
Application ELISA | Reactivity Expected | Dilution info 1/10000 - 1/50000 | Notes - |
Application ICC/IF | Reactivity Expected | Dilution info 1/1000 - 1/5000 | Notes - |
Application Dot | Reactivity Reacts | Dilution info - | Notes - |
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Suitable for Flow Cyt, IHC-Fr, IHC-P, IM, ELISA, ICC/IF, Dot. Preadsorbed to minimise non-specific binding and high background staining.
No reaction was observed against Bovine, Chicken, Goat, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins.
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Terms & Conditions.
Dot blot analysis of ab7135. Imaged with BioRad ChemiDoc, Fluorescein filter.
All lanes: Dot Blot - Goat Anti-Guinea pig IgG H&L (FITC) preadsorbed (ab7135)
Lane 1: Guinea Pig IgG at 100 ng
Lane 2: Guinea Pig IgG at 33.3 ng
Lane 3: Guinea Pig IgG at 11.1 ng
Lane 4: Guinea Pig IgG at 3.7 ng
Lane 5: Guinea Pig IgG at 1.23 ng
All lanes: Goat Anti-Guinea Pig IgG Antibody FITC at 1 µg/mL
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