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AB7135

Goat Anti-Guinea pig IgG H&L (FITC) preadsorbed

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Suitable for Flow Cyt, IHC-Fr, IHC-P, IM, ELISA, ICC/IF, Dot. Preadsorbed to minimise non-specific binding and high background staining.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

1 Images
Dot Blot - Goat Anti-Guinea pig IgG H&L (FITC) preadsorbed (AB7135)
  • Dot

Supplier Data

Dot Blot - Goat Anti-Guinea pig IgG H&L (FITC) preadsorbed (AB7135)

Dot blot analysis of ab7135. Imaged with BioRad ChemiDoc, Fluorescein filter.

All lanes:

Dot Blot - Goat Anti-Guinea pig IgG H&L (FITC) preadsorbed (ab7135)

Lane 1:

Guinea Pig IgG at 100 ng

Lane 2:

Guinea Pig IgG at 33.3 ng

Lane 3:

Guinea Pig IgG at 11.1 ng

Lane 4:

Guinea Pig IgG at 3.7 ng

Lane 5:

Guinea Pig IgG at 1.23 ng

Secondary

All lanes:

Goat Anti-Guinea Pig IgG Antibody FITC at 1 µg/mL

false

  • Unconjugated

    Goat Anti-Guinea pig IgG H&L

  • Alkaline Phosphatase

    Goat Anti-Guinea pig IgG H&L (Alkaline Phosphatase) preadsorbed

  • 572 TRITC

    Goat Anti-Guinea pig IgG H&L (TRITC)

  • 615 Texas Red®

    Goat Anti-Guinea pig IgG H&L (Texas Red ®)

  • 596 Cy3.5®

    Goat Anti-Guinea pig IgG H&L (Cy3.5 ®) preadsorbed

  • 10nm Gold

    Goat Anti-Guinea pig IgG H&L (10nm Gold)

  • Biotin

    Goat Anti-Guinea pig IgG H&L (Biotin)

  • HRP

    Goat Anti-Guinea pig IgG H&L (HRP)

  • 518 DyLight® 488

    Goat Anti-Guinea pig IgG H&L (DyLight® 488)

  • 618 DyLight® 594

    Goat Anti-Guinea pig IgG H&L (DyLight® 594)

  • 576 DyLight® 550

    Goat Anti-Guinea pig IgG H&L (DyLight® 550) preadsorbed

  • 565 Cy3®

    Goat Anti-Guinea pig IgG H&L (Cy3 ®) preadsorbed

  • 519 Alexa Fluor® 488

    Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 488)

  • 565 Alexa Fluor® 555

    Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 555)

  • 775 Alexa Fluor® 750

    Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 750)

  • 603 Alexa Fluor® 568

    Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 568)

  • 421 Alexa Fluor® 405

    Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 405)

  • 665 Alexa Fluor® 647

    Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 647)

  • 617 Alexa Fluor® 594

    Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 594)

  • 667 Cy5®

    Goat Anti-Guinea pig IgG H&L (Cy5 ®) preadsorbed

  • 673 DyLight® 650

    Goat Anti-Guinea pig IgG H&L (DyLight® 650) preadsorbed

Key facts

Host species

Goat

Target species

Guinea pig

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Sheep, Rabbit, Goat, Horse, Chicken, Hamster, Cow, Human

Pre-adsorbed

Yes

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Applications

Flow Cyt, IHC-P, ELISA, ICC/IF, IM, Dot, IHC-Fr

applications

Clonality

Polyclonal

Specificity

No reaction was observed against Bovine, Chicken, Goat, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/10000 - 1/50000", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"<p></p>" }, "Dot": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage buffer
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

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Target data

See full target information Guinea pig IgG
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Product promise

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