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Suitable for Flow Cyt, IHC-Fr, IHC-P, IM, ELISA, ICC/IF, Dot. Preadsorbed to minimise non-specific binding and high background staining.

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Images

Dot Blot - Goat Anti-Guinea pig IgG H&L (FITC) preadsorbed (AB7135), expandable thumbnail

Key facts

Host species
Goat
Target species
Guinea pig
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Mouse, Rat, Sheep, Rabbit, Goat, Horse, Chicken, Hamster, Cow, Human
Conjugation
FITC
Excitation/Emission
Ex: 495nm, Em: 519nm
Applications
Flow Cyt, IHC-Fr, IHC-P, IM, ELISA, ICC/IF, Dot
Clonality
Polyclonal

Reactivity data

Application
Flow Cyt
Reactivity
Expected
Dilution info
-
Notes

-

Application
IHC-Fr
Reactivity
Expected
Dilution info
-
Notes

-

Application
IHC-P
Reactivity
Expected
Dilution info
-
Notes

-

Application
IM
Reactivity
Expected
Dilution info
-
Notes

-

Application
ELISA
Reactivity
Expected
Dilution info
1/10000 - 1/50000
Notes

-

Application
ICC/IF
Reactivity
Expected
Dilution info
1/1000 - 1/5000
Notes

-

Application
Dot
Reactivity
Reacts
Dilution info
-
Notes

-

Alternative names

Recommended products

Suitable for Flow Cyt, IHC-Fr, IHC-P, IM, ELISA, ICC/IF, Dot. Preadsorbed to minimise non-specific binding and high background staining.

Key facts

Description
Goat Anti-Guinea pig IgG H&L (FITC) preadsorbed
Host species
Goat
Target species
Guinea pig
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Mouse, Rat, Sheep, Rabbit, Goat, Horse, Chicken, Hamster, Cow, Human
Conjugation
FITC
Excitation/Emission
Ex: 495nm, Em: 519nm
Applications
Flow Cyt, IHC-Fr, IHC-P, IM, ELISA, ICC/IF, Dot
Clonality
Polyclonal
Pre-adsorbed
Yes
Specificity

No reaction was observed against Bovine, Chicken, Goat, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins.

Concentration
Loading...

Properties

Form
Liquid
Storage buffer

Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Purification technique
Affinity purification
Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Dot Blot - Goat Anti-Guinea pig IgG H&L (FITC) preadsorbed (ab7135), expandable thumbnail

    Dot Blot - Goat Anti-Guinea pig IgG H&L (FITC) preadsorbed (ab7135)

    Dot blot analysis of ab7135. Imaged with BioRad ChemiDoc, Fluorescein filter.

    All lanes: Dot Blot - Goat Anti-Guinea pig IgG H&L (FITC) preadsorbed (ab7135)

    Lane 1: Guinea Pig IgG at 100 ng

    Lane 2: Guinea Pig IgG at 33.3 ng

    Lane 3: Guinea Pig IgG at 11.1 ng

    Lane 4: Guinea Pig IgG at 3.7 ng

    Lane 5: Guinea Pig IgG at 1.23 ng

    Secondary

    All lanes: Goat Anti-Guinea Pig IgG Antibody FITC at 1 µg/mL

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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