AB96998
Goat Anti-Human IgA alpha chain (DyLight® 650)
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(5 Publications)
Suitable for ICC/IF, Flow Cyt, IHC-P. Cited in 5 publications.
View Alternative Names
Immunoglobulin heavy constant alpha 1, Ig alpha-1 chain C region, Ig alpha-1 chain C region BUR, Ig alpha-1 chain C region TRO, IGHA1, Immunoglobulin heavy constant alpha 2, Ig alpha-2 chain C region, Ig alpha-2 chain C region BUT, Ig alpha-2 chain C region LAN, IGHA2
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Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"<p>DyLight<sup>®</sup> 650 replaces DyLight<sup>®</sup> 649. The DyLight<sup>®</sup> 650 excitation is 652nm and the emission is 672nm. DyLight<sup>®</sup> 650 hence provides the same far-red fluorescence and photostability as the DyLight<sup>®</sup> 649 dye. Please refer to the vial in order to check whether the product you received is DyLight<sup>®</sup> 649 or DyLight<sup>®</sup> 650. This information however will have no impact on your experiments.</p>" } } }
Product details
DyLight® 650 replaces DyLight® 649.
Properties and storage information
Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Target data
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268). Ig alpha is the major immunoglobulin class in body secretions (PubMed : 2241915).
Additional targets
IGHA2
Publications (5)
Recent publications for all applications. Explore the full list and refine your search
Nature communications 15:6712 PubMed39112489
2024
Profiling IgG and IgA antibody responses during vaccination and infection in a high-risk gonorrhoea population.
Applications
Unspecified application
Species
Unspecified reactive species
Lenka Stejskal,Angela Thistlethwaite,Fidel Ramirez-Bencomo,Smruti Rashmi,Odile Harrison,Ian M Feavers,Martin C J Maiden,Ann Jerse,Grace Barnes,Oscar Chirro,James Chemweno,Eunice Nduati,Ana Cehovin,Christoph Tang,Eduard J Sanders,Jeremy P Derrick
iScience 26:105991 PubMed36824270
2023
The gut microbiota of people with asthma influences lung inflammation in gnotobiotic mice.
Applications
Unspecified application
Species
Unspecified reactive species
Naomi G Wilson,Ariel Hernandez-Leyva,Anne L Rosen,Natalia Jaeger,Ryan T McDonough,Jesus Santiago-Borges,Michael A Lint,Thomas R Rosen,Christopher P Tomera,Leonard B Bacharier,S Joshua Swamidass,Andrew L Kau
BioEssays : news and reviews in molecular, cellular and developmental biology 43:e2000257 PubMed33377226
2020
Microscopy-based assay for semi-quantitative detection of SARS-CoV-2 specific antibodies in human sera: A semi-quantitative, high throughput, microscopy-based assay expands existing approaches to measure SARS-CoV-2 specific antibody levels in human sera.
Applications
Unspecified application
Species
Unspecified reactive species
Constantin Pape,Roman Remme,Adrian Wolny,Sylvia Olberg,Steffen Wolf,Lorenzo Cerrone,Mirko Cortese,Severina Klaus,Bojana Lucic,Stephanie Ullrich,Maria Anders-Össwein,Stefanie Wolf,Berati Cerikan,Christopher J Neufeldt,Markus Ganter,Paul Schnitzler,Uta Merle,Marina Lusic,Steeve Boulant,Megan Stanifer,Ralf Bartenschlager,Fred A Hamprecht,Anna Kreshuk,Christian Tischer,Hans-Georg Kräusslich,Barbara Müller,Vibor Laketa
Cell : PubMed32991843
2020
Mapping Systemic Inflammation and Antibody Responses in Multisystem Inflammatory Syndrome in Children (MIS-C).
Applications
Unspecified application
Species
Unspecified reactive species
Conor N Gruber,Roosheel S Patel,Rebecca Trachtman,Lauren Lepow,Fatima Amanat,Florian Krammer,Karen M Wilson,Kenan Onel,Daniel Geanon,Kevin Tuballes,Manishkumar Patel,Konstantinos Mouskas,Timothy O'Donnell,Elliot Merritt,Nicole W Simons,Vanessa Barcessat,Diane M Del Valle,Samantha Udondem,Gurpawan Kang,Sandeep Gangadharan,George Ofori-Amanfo,Uri Laserson,Adeeb Rahman,Seunghee Kim-Schulze,Alexander W Charney,Sacha Gnjatic,Bruce D Gelb,Miriam Merad,Dusan Bogunovic
Nature 534:263-6 PubMed27279225
2016
Development of the gut microbiota and mucosal IgA responses in twins and gnotobiotic mice.
Applications
Unspecified application
Species
Unspecified reactive species
Joseph D Planer,Yangqing Peng,Andrew L Kau,Laura V Blanton,I Malick Ndao,Phillip I Tarr,Barbara B Warner,Jeffrey I Gordon
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