Goat Anti-Human IgG (Fab')2 (HRP) is a secondary antibody for chemiluminescent detection. Ideal for Western blot. Suitable for Western blot and ELISA.
- Reacts specifically with the Fab and F(ab')2 portion of human IgG
- Cited in over 5 publications
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- mAbs 15:22817632023Anti-citrullinated histone monoclonal antibody CIT-013, a dual action therapeutic for neutrophil extracellular trap-associated autoimmune diseases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaarten van der Linden,Sangeeta Kumari,Daphne Montizaan,Stephanie van Dalen,Annemarie Kip,Martyn Foster,Inge Reinieren-Beeren,Elsa Neubert,Luise Erpenbeck,Kelsy Waaijenberg,Tirza Bruurmijn,Rezie Te Poele,Peter van Zandvoort,Paul Vink,Eric Meldrum,Helmuth van Es,Renato G S ChiriviPubMed 38031350
- Life sciences 320:1215252023Enhancement of immunogenicity and neutralizing responses against SARS-CoV-2 spike protein using the Fc fusion fragment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYahya Ehteshaminia,Seyedeh Farzaneh Jalali,Farhad Jadidi-Niaragh,Seyed Ehsan Enderami,Abdol Sattar Pagheh,Esmaeil Akbari,Saeid Abedian Kenari,Hadi HassanniaPubMed 36841470
- NPJ vaccines 7:1692022Effective vaccination strategy using SARS-CoV-2 spike cocktail against Omicron and other variants of concern.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuan Shi,Gang Wang,Jian Zheng,Abhishek K Verma,Xiaoqing Guan,Moffat M Malisheni,Qibin Geng,Fang Li,Stanley Perlman,Lanying DuPubMed 36535987
- Translational oncology 21:1014242022A novel multifunctional anti-PD-L1-CD16a-IL15 induces potent cancer cell killing in PD-L1-positive tumour cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYumei Li,Lingjun Wu,Yueying Liu,Siwen Ma,Biyi Huang,Xianjing Feng,Hui WangPubMed 35477065
- mAbs 10:1060-10722018Massive parallel screening of phage libraries for the generation of repertoires of human immunomodulatory monoclonal antibodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmanuele Sasso,Chiara D'Avino,Margherita Passariello,Anna Morena D'Alise,Daniela Siciliano,Maria Luisa Esposito,Guendalina Froechlich,Riccardo Cortese,Elisa Scarselli,Nicola Zambrano,Alfredo Nicosia,Claudia De LorenzoPubMed 29995563
- PloS one 12:e01778102017Development of a versatile oncolytic virus platform for local intra-tumoural expression of therapeutic transgenes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNalini Marino,Sam Illingworth,Prithvi Kodialbail,Ashvin Patel,Hugo Calderon,Rochelle Lear,Kerry D Fisher,Brian R Champion,Alice C N BrownPubMed 28542292
- Proceedings of the National Academy of Sciences of 111:10209-142014Poly(ADP-ribose) polymerase-dependent energy depletion occurs through inhibition of glycolysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShaida A Andrabi et. al.PubMed 24987120
Key facts
- Host species
- Goat
- Target species
- Human
- Target isotype
- IgG
- Target specificity
- F(ab')2
- Conjugation
- HRP
- Applications
- ELISA, WB
- Clonality
- Polyclonal
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
Target data
Function
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). Mediates IgG effector functions on monocytes triggering ADCC of virus-infected cells.
Additional Targets
, ,
Alternative names
Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1
Recommended products
Goat Anti-Human IgG (Fab')2 (HRP) is a secondary antibody for chemiluminescent detection. Ideal for Western blot. Suitable for Western blot and ELISA.
- Reacts specifically with the Fab and F(ab')2 portion of human IgG
- Cited in over 5 publications
Key facts
- Description
- Goat Anti-Human IgG (Fab')2 (HRP)
- Host species
- Goat
- Target species
- Human
- Target isotype
- IgG
- Target specificity
- F(ab')2
- Conjugation
- HRP
- Applications
- ELISA, WB
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Specificity
ab87422 is specific for the Fab and F(ab')2 portion of human IgG by IEP. It reacts with other human immunoglobulins through common light chain reactivity.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 0.1% BSA- Purification technique
- Affinity purification Protein G
- Purification notes
This antibody has been cross adsorbed against the Fc domain and will react with less than 1 % of the Fc domain of human IgG.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
This antibody reacts with the (Fab')2 region of Human IgG
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1 product image
Marino et al PLoS One. 2017 May 18;12(5):e0177810. doi: 10.1371/journal.pone.0177810. eCollection 2017. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/ELISA - Goat Anti-Human IgG (Fab')2 (HRP) (ab87422)
Characterisation of virus activity and functional expression of monoclonal antibodies.(Panel D) IgG1 antibody expression, assessed by anti-IgG1 ELISA, in cellular supernatants of carcinoma cell lines infected for 24-96hrs with NG-135 at 1ppc.
Media was removed from infected or control cells, clarified by centrifugation and diluted 1 in 2 in 3% BSA/PBS for storage at -20°C. For quantification of human IgG1, 96 well plates were coated overnight, 4°C, with mouse monoclonal anti-human IgG1 Fc antibody (Mouse monoclonal [2C11] Anti-Human IgG1 Fc ab1927, Abcam) at a 1:1000 dilution in carbonate/bicarbonate buffer. Plates were blocked in 3% BSA/PBS, washed and samples and standards added to the plate for 1 hr and RT. Secondary detection was carried out using a HRP conjugated goat anti-human IgG Fab (ab87422, Abcam) incubated for 1 hr, RT.
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