Suitable for ICC, IHC-P, ELISA, WB. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 31 publications.
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- eLife 12:2023CTLA-4 antibody-drug conjugate reveals autologous destruction of B-lymphocytes associated with regulatory T cell impairment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMusleh M Muthana,Xuexiang Du,Mingyue Liu,Xu Wang,Wei Wu,Chunxia Ai,Lishan Su,Pan Zheng,Yang LiuPubMed 38127423
- Nature communications 14:59482023Monkeypox virus-infected individuals mount comparable humoral immune responses as Smallpox-vaccinated individuals.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAshley D Otter,Scott Jones,Bethany Hicks,Daniel Bailey,Helen Callaby,Catherine Houlihan,Tommy Rampling,Nicola Claire Gordon,Hannah Selman,Panayampalli S Satheshkumar,Michael Townsend,Ravi Mehta,Marcus Pond,Rachael Jones,Deborah Wright,Clarissa Oeser,Simon Tonge,Ezra Linley,Georgia Hemingway,Tom Coleman,Sebastian Millward,Aaron Lloyd,Inger Damon,Tim Brooks,Richard Vipond,Cathy Rowe,Bassam HallisPubMed 37741831
- mAbs 15:22320872023Improved antibody pharmacokinetics by disruption of contiguous positive surface potential and charge reduction using alternate human framework.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRomain Ollier,Aline Fuchs,Florence Gauye,Katarzyna Piorkowska,Sébastien Menant,Monisha Ratnam,Paolo Montanari,Florence Guilhot,Didier Phillipe,Mickael Audrain,Anne-Laure Egesipe,Damien Névoltris,Tamara Seredenina,Andrea Pfeifer,Marie Kosco-Vilbois,Tariq AfrozPubMed 37408314
- Frontiers in cellular and infection microbiology 13:12022762023A conserved epitope in VAR2CSA is targeted by a cross-reactive antibody originating from Duffy binding protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesUwa Iyamu,Daniel Ferrer Vinals,Bernard Tornyigah,Eliana Arango,Rakesh Bhat,Trixie Rae Adra,Simranjit Grewal,Kimberly Martin,Amanda Maestre,Michael Overduin,Bart Hazes,Stephanie K YanowPubMed 37396303
- Nature medicine 29:1448-14552023Propagative α-synuclein seeds as serum biomarkers for synucleinopathies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAyami Okuzumi,Taku Hatano,Gen Matsumoto,Shuko Nojiri,Shin-Ichi Ueno,Yoko Imamichi-Tatano,Haruka Kimura,Soichiro Kakuta,Akihide Kondo,Takeshi Fukuhara,Yuanzhe Li,Manabu Funayama,Shinji Saiki,Daisuke Taniguchi,Taiji Tsunemi,Deborah McIntyre,Jean-Jacques Gérardy,Michel Mittelbronn,Rejko Kruger,Yasuo Uchiyama,Nobuyuki Nukina,Nobutaka HattoriPubMed 37248302
- Journal of neuroinflammation 20:742023Decrease in naturally occurring antibodies against epitopes of Alzheimer's disease (AD) risk gene products is associated with cognitive decline in AD.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongmei Gu,Luchun Wang,Nan Zhang,Huali Wang,Xin YuPubMed 36922858
- Frontiers in neurology 14:11307482023Further analysis of natural antibodies against ischemic stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingjing Qi,Quanhang Jiang,Peng Wang,Zhenqi Wang,Xuan ZhangPubMed 36741286
- Nature communications 14:172023The rapid and highly parallel identification of antibodies with defined biological activities by SLISY.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSteve Lu,Austin K Mattox,P Aitana Azurmendi,Ilias Christodoulou,Katharine M Wright,Maria Popoli,Zan Chen,Surojit Sur,Yana Li,Challice L Bonifant,Chetan Bettegowda,Nickolas Papadopoulos,Shibin Zhou,Sandra B Gabelli,Bert Vogelstein,Kenneth W KinzlerPubMed 36596784
- Frontiers in neurology 13:10377772022The SFT2D2 gene is associated with the autoimmune pathology of schizophrenia in a Chinese population.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDuilin Liu,Lin Wu,Hui Wei,Caiyun Zhu,Runhui Tian,Wanwan Zhu,Qi XuPubMed 36619926
- Journal of bacteriology 204:e00335222022The Sia System and c-di-GMP Play a Crucial Role in Controlling Cell-Association of Psl in Planktonic P. aeruginosa.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulia E Dreifus,Lindsey O'Neal,Holly M Jacobs,Adithya S Subramanian,P Lynne Howell,Daniel J Wozniak,Matthew R ParsekPubMed 36448788
Key facts
- Host species
Goat
- Target species
Human
- Target isotype
IgG
- Target specificity
Fc region
- Minimal cross-reactivity
Mouse, Rat, Sheep, Rabbit, Goat, Horse, Chicken, Cow
- Conjugation
HRP
- Applications
ICC, IHC-P, ELISA, WB
- Clonality
Polyclonal
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/200.00000 - 1/2000.00000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/10000.00000 - 1/100000.00000 | Notes (Primary). |
Application WB | Reactivity Reacts | Dilution info 1/2000.00000 - 1/25000.00000 | Notes (Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000). |
Target data
Function
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).
Targets
Immunoglobulin heavy constant gamma 2, Immunoglobulin heavy constant gamma 3, Immunoglobulin heavy constant gamma 4
Alternative names
Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1
Recommended products
Suitable for ICC, IHC-P, ELISA, WB. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 31 publications.
Key facts
- Description
Goat Anti-Human IgG Fc (HRP) preadsorbed
- Host species
Goat
- Target species
Human
- Target isotype
IgG
- Target specificity
Fc region
- Minimal cross-reactivity
Mouse, Rat, Sheep, Rabbit, Goat, Horse, Chicken, Cow
- Conjugation
HRP
- Applications
ICC, IHC-P, ELISA, WB
- Clonality
Polyclonal
- Pre-adsorbed
Yes
- Isotype
IgG
- Concentration
Properties
- Form
Liquid
- Storage buffer
pH: 6.8 - 7.4
Constituents: PBS, 0.2% BSA, 0.00063% 2-methyl-4-isothiazolin-3-one, 0.00063% 5-chloro-2-methyl-4-isothiazolin-3-one- Purification technique
Affinity purification Immunogen
- Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Product promise
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9 product images
Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624)
This data was developed using Anti-Myc tag antibody [Hyper-myc] ab289981, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concnetration are 5% NFDM/TBST.
Anti-Myc tag antibody [Hyper-myc] ab289981 detects a ranged of myc tagged proteins in WB.
All lanes: Western blot - Anti-Myc tag antibody [Hyper-myc] (Anti-Myc tag antibody [Hyper-myc] ab289981) at 1/5000 dilution
Lane 1: HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 1 µg
Lane 2: HEK-293T transfected with cas9 ( S. Aureas) expression vector containing a myc-His-tag®, whole cell lysate at 1 µg
Lane 3: HEK-293T transfected with human SNCA140 expression vector containing a myc-His-tag®, whole cell lysate at 1 µg
Lane 4: HEK-293T transfected with human IDS expression vector containing a myc-His-tag®, whole cell lysate at 1 µg
Lane 5: HEK-293T transfected with human NF2 S518A expression vector containing a myc-His-tag®, whole cell lysate at 1 µg
Lane 6: HEK-293T transfected with human Alpha-synuclein expression vector containing a myc-His-tag®, whole cell lysate at 1 µg
SecondaryAll lanes: Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1/10000 dilution
Predicted band size: 49 kDa
Exposure time: 1s
Indirect ELISA - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624)
Indirect ELISA showing primary antibody Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [EPR24852-116] - Human IgG1 (Chimeric) ab323000 binding to Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera) ab272105). Plates were coated with Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera) ab272105), Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera, Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera) ab272106) and Recombinant human coronavirus SARS spike glycoprotein (Tagged, Recombinant Human coronavirus SARS spike glycoprotein (Tagged) ab270844) at 1000 ng/ml. Binding of Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [EPR24852-116] - Human IgG1 (Chimeric) ab323000 was assessed in a serial dilution range 0.016- 1000 ng/mL (a 3-fold serial dilution). Secondary antibody, Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) was used at 1:5000.
Indirect ELISA - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624)
Indirect ELISA showing primary antibody Anti-SARS-CoV-2 Spike Glycoprotein S1 [8B12-C2] – Human IgG1 (Chimeric) ab322272 binding to Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera) ab272105). Plates were coated with Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera) ab272105) and Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera, Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera) ab272106) at 1000 ng/ml. Binding of Anti-SARS-CoV-2 Spike Glycoprotein S1 [8B12-C2] – Human IgG1 (Chimeric) ab322272 was assessed in a serial dilution range 0.016- 1000 ng/mL (a 3-fold serial dilution). Secondary antibody, Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) was used at 1:5000.
Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624)
False colour image of Western blot: Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] - Human IgG1 (Chimeric) (Anti-SARS-CoV-2 Spike Glycoprotein S1 [8B12-C2] – Human IgG1 (Chimeric) ab322272) staining at 1/500 dilution, shown in black; Mouse Monoclonal 6X His tag® (Anti-6X His tag® antibody [HIS.H8] ab18184) loading control staining at 1/200 dilution, shown in red. In Western blot, Anti-SARS-CoV-2 Spike Glycoprotein S1 [8B12-C2] – Human IgG1 (Chimeric) ab322272 was shown to bind specifically to SARS-CoV and SARS-CoV-2 Spike Glycoproteins. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1:10000 and Goat anti-Mouse IgG H&L 680RD at 1:20000.
All lanes: Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 [8B12-C2] – Human IgG1 (Chimeric) (Anti-SARS-CoV-2 Spike Glycoprotein S1 [8B12-C2] – Human IgG1 (Chimeric) ab322272) at 1/500 dilution
Lane 1: Western blot - Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) (Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) ab273068) at 0.2 µg
Lane 2: SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His Recombinant Protein at 0.2 µg
Lane 3: SARS-CoV Spike S1+S2 ECD-His Recombinant Protein at 0.2 µg
SecondaryLanes 1 - 3: Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1/10000 dilution
Lanes 1 - 3: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 120-160 kDa
Exposure time: 5s
Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624)
All lanes: Western blot - Anti-HMGB1 antibody [EPR21207] (Anti-HMGB1 antibody [EPR21207] ab213256) at 1/100 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 5: parental HAP1(HOWT01) (human chronic myelogenous leukemia near-haploid cell ) whole cell lysate at 20 µg
Lane 6: HMGB1 knockout HAP1 whole cell lysate at 20 µg
Lane 7: MEF (mouse embryo fibroblast) whole cell lysate at 20 µg
Lane 8: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 9: Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1/10000 dilution
Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624)
All lanes: Western blot - Anti-PLBD2 antibody [IGX4128H] (Anti-PLBD2 antibody [IGX4128H] ab213295) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse heart tissue lysate at 20 µg
Lane 3: Human cerebellum tissue lysate at 20 µg
Lane 4: Rat brain tissue lysate at 20 µg
Lane 5: Rat heart tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1/10000 dilution
Exposure time: 4s
Dot Blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624)
Dot blot analysis of bat SARS-like coronavirus RsSHC014 Spike glycoprotein using Anti-bat SARS-like coronavirus RsSHC014 Spike glycoprotein antibody [abd166] ab317329 at 1:1000 followed by a Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1:10,000 dilution.
All lanes: Dot Blot - Anti-bat SARS-like coronavirus RsSHC014 Spike glycoprotein antibody [abd166] (Anti-bat SARS-like coronavirus RsSHC014 Spike glycoprotein antibody [abd166] ab317329) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containing a myc-his tag whole cell lysate
Lane 2: 293T cells transfected with a Bat Spike glycoprotein expression vector containing a myc-his-tag whole cell lysate
Lane 3: His-tagged bat SARS-like coronavirus RsSHC01 recombinant fragment protein
SecondaryAll lanes: Dot Blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1/10000 dilution
Exposure time: 180s
Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624)
Lane 1: Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active, His tagged) (Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) ab273068) at 0.2 ug
Lane 2: Recombinant SARS-COV-2 NSP2 protein (His tagged) at 0.2 ug
Performed under reducing conditions.
Observed band size: 135 kDa.
False colour image of Western blot: Rabbit monoclonal [EPR24852-116] to SARS-CoV-2 Spike Glycoprotein S1 - Human IgG1 (Chimeric), Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [EPR24852-116] - Human IgG1 (Chimeric) ab323000 staining at 1/500 dilution, shown in black; Mouse Monoclonal 6X His tag® (Anti-6X His tag® antibody [HIS.H8] ab18184) loading control staining at 1/1000 dilution, shown in red. In Western blot, Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [EPR24852-116] - Human IgG1 (Chimeric) ab323000 was shown to bind specifically to SARS-CoV-2 spike glycoprotein S1. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1:10000 and Goat anti-Mouse IgG H&L 680RD at 1:20000.
Blocking buffer: 3% milk in TBS-0.1% Tween® 20 (TBS-T).
Exposure time: 30 Sec.
All lanes: Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [EPR24852-116] - Human IgG1 (Chimeric) (Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [EPR24852-116] - Human IgG1 (Chimeric) ab323000) at 1/500 dilution
Lane 1: Western blot - Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) (Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) ab273068) at 0.2 µg
Lane 2: Recombinant SARS-COV-2 NSP2 protein (His tagged) at 0.2 µg
SecondaryLanes 1 - 2: Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1/10000 dilution
Lanes 1 - 2: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 135 kDa
Exposure time: 30s
Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624)
Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
Exposure Time: Lane 1: 3 seconds, Lane 2-3: 20 seconds.
Negative control: Skeletal muscle.
The identity of the bands between 27 kDa and 150 kDa are unknown.
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a loading control.All lanes: Western blot - Anti-TAGLN/Transgelin antibody [EPR21206] (Anti-TAGLN/Transgelin antibody [EPR21206] ab213273) at 1/500 dilution
Lane 1: Rat bladder tissue lysate at 20 µg
Lane 2: Rat colon tissue lysate at 20 µg
Lane 3: Rat skeletal muscle tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1/10000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
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