Goat Anti-Human IgG Fc (HRP) preadsorbed

• WB

Supplier Data

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (AB98624)

This data was developed using ab289981, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concnetration are 5% NFDM/TBST.

ab289981 detects a ranged of myc tagged proteins in WB.

All lanes:

Western blot - Anti-Myc tag antibody [Hyper-myc] (<a href='/en-us/products/primary-antibodies/myc-tag-antibody-hyper-myc-ab289981'>ab289981</a>) at 1/5000 dilution

Lane 1:

HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 1 µg

Lane 2:

HEK-293T transfected with cas9 ( S. Aureas) expression vector containing a myc-His-tag®, whole cell lysate at 1 µg

Lane 3:

HEK-293T transfected with human SNCA140 expression vector containing a myc-His-tag®, whole cell lysate at 1 µg

Lane 4:

HEK-293T transfected with human IDS expression vector containing a myc-His-tag®, whole cell lysate at 1 µg

Lane 5:

HEK-293T transfected with human NF2 S518A expression vector containing a myc-His-tag®, whole cell lysate at 1 µg

Lane 6:

HEK-293T transfected with human Alpha-synuclein expression vector containing a myc-His-tag®, whole cell lysate at 1 µg

Secondary

All lanes:

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1/10000 dilution

Predicted band size: 49 kDa

false

Exposure time: 1s

• WB

Lab

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (AB98624)

All lanes:

Western blot - Anti-HMGB1 antibody [EPR21207] (<a href='/en-us/products/primary-antibodies/hmgb1-antibody-epr21207-ab213256'>ab213256</a>) at 1/100 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

parental HAP1(HOWT01) (human chronic myelogenous leukemia near-haploid cell ) whole cell lysate at 20 µg

Lane 6:

HMGB1 knockout HAP1 whole cell lysate at 20 µg

Lane 7:

MEF (mouse embryo fibroblast) whole cell lysate at 20 µg

Lane 8:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 9:

Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1/10000 dilution

false

• WB

Supplier Data

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (AB98624)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 27043630).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-Rho A + B + C antibody [EPR18299] staining at 1/1000 dilution.

All lanes:

Western blot - Anti-RhoA + RhoB (ADP-ribosylated) antibody [VIF140-A1] (<a href='/en-us/products/primary-antibodies/rhoa-rhob-adp-ribosylated-antibody-vif140-a1-ab324028'>ab324028</a>) at 1/1000 dilution

Lane 1:

Untreated HT-22 (mouse hippocampal neuronal cell) whole cell lysate at 20 µg

Lane 2:

HT-22 treated with 500nM C3 for 6 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1/10000 dilution

Observed band size: 22 kDa,36 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (AB98624)

Blocking buffer and concentration : 5% NFDM/TBST  Diluting buffer and concentration : 5% NFDM/TBST Exposure Time : Lane 1 : 3 seconds, Lane 2-3 : 20 seconds. Negative control : Skeletal muscle. The identity of the bands between 27 kDa and 150 kDa are unknown. ab181602 was used as a loading control.

All lanes:

Western blot - Anti-TAGLN/Transgelin antibody [EPR21206] (<a href='/en-us/products/primary-antibodies/tagln-transgelin-antibody-epr21206-ab213273'>ab213273</a>) at 1/500 dilution

Lane 1:

Rat bladder tissue lysate at 20 µg

Lane 2:

Rat colon tissue lysate at 20 µg

Lane 3:

Rat skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1/10000 dilution

Predicted band size: 23 kDa

Observed band size: 23 kDa

false

• WB

Lab

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (AB98624)

Blocking and diluting buffer was 5% NFDM/TBST

All lanes:

Western blot - Anti-PLBD2 antibody [IGX4128H] (<a href='/en-us/products/primary-antibodies/plbd2-antibody-igx4128h-ab213295'>ab213295</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Human cerebellum tissue lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Lane 5:

Rat heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1/10000 dilution

false

Exposure time: 4s

• I-ELISA

Lab

Indirect ELISA - Goat Anti-Human IgG Fc (HRP) preadsorbed (AB98624)

Indirect ELISA showing primary antibody ab323000 binding to Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, ab272105). Plates were coated with Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, ab272105), Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera, ab272106) and Recombinant human coronavirus SARS spike glycoprotein (Tagged, ab270844) at 1000 ng/ml. Binding of ab323000 was assessed in a serial dilution range 0.016- 1000 ng/mL (a 3-fold serial dilution). Secondary antibody, Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) was used at 1 : 5000.

• I-ELISA

Lab

Indirect ELISA - Goat Anti-Human IgG Fc (HRP) preadsorbed (AB98624)

Indirect ELISA showing primary antibody ab322272 binding to Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, ab272105). Plates were coated with Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, ab272105) and Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera, ab272106) at 1000 ng/ml. Binding of ab322272 was assessed in a serial dilution range 0.016- 1000 ng/mL (a 3-fold serial dilution). Secondary antibody, Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) was used at 1 : 5000.

• WB

Lab

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (AB98624)

False colour image of Western blot : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] - Human IgG1 (Chimeric) (ab322272) staining at 1/500 dilution, shown in black; Mouse Monoclonal 6X His tag® (ab18184) loading control staining at 1/200 dilution, shown in red. In Western blot, ab322272 was shown to bind specifically to SARS-CoV and SARS-CoV-2 Spike Glycoproteins. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1 : 10000 and Goat anti-Mouse IgG H&L 680RD at 1 : 20000.

All lanes:

Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 [8B12-C2] – Human IgG1 (Chimeric) (<a href='/en-us/products/primary-antibodies/sars-cov-2-spike-glycoprotein-s1-8b12-c2-human-igg1-chimeric-ab322272'>ab322272</a>) at 1/500 dilution

Lane 1:

Western blot - Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) (<a href='/en-us/products/proteins-peptides/recombinant-human-coronavirus-sars-cov-2-spike-glycoprotein-s1-active-ab273068'>ab273068</a>) at 0.2 µg

Lane 2:

SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His Recombinant Protein at 0.2 µg

Lane 3:

SARS-CoV Spike S1+S2 ECD-His Recombinant Protein at 0.2 µg

Secondary

Lanes 1 - 3:

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1/10000 dilution

Lanes 1 - 3:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 120-160 kDa

false

Exposure time: 5s

• WB

Lab

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (AB98624)

Lane 1 : Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active, His tagged) (ab273068) at 0.2 ug

Lane 2 : Recombinant SARS-COV-2 NSP2 protein (His tagged) at 0.2 ug

Performed under reducing conditions.

Observed band size : 135 kDa.

False colour image of Western blot : Rabbit monoclonal [EPR24852-116] to SARS-CoV-2 Spike Glycoprotein S1 - Human IgG1 (Chimeric), ab323000 staining at 1/500 dilution, shown in black; Mouse Monoclonal 6X His tag® (ab18184) loading control staining at 1/1000 dilution, shown in red. In Western blot, ab323000 was shown to bind specifically to SARS-CoV-2 spike glycoprotein S1. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1 : 10000 and Goat anti-Mouse IgG H&L 680RD at 1 : 20000.

Blocking buffer : 3% milk in TBS-0.1% Tween® 20 (TBS-T).

Exposure time : 30 Sec.

All lanes:

Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [EPR24852-116] - Human IgG1 (Chimeric) (<a href='/en-us/products/primary-antibodies/sars-cov-2-spike-glycoprotein-s1-antibody-epr24852-116-human-igg1-chimeric-ab323000'>ab323000</a>) at 1/500 dilution

Lane 1:

Western blot - Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) (<a href='/en-us/products/proteins-peptides/recombinant-human-coronavirus-sars-cov-2-spike-glycoprotein-s1-active-ab273068'>ab273068</a>) at 0.2 µg

Lane 2:

Recombinant SARS-COV-2 NSP2 protein (His tagged) at 0.2 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1/10000 dilution

Lanes 1 - 2:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 135 kDa

false

Exposure time: 30s

• WB

Supplier Data

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (AB98624)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 27043630).

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

In Western blot, Anti-Rho A + B + C antibody [EPR18299] staining at 1/1000 dilution.

All lanes:

Western blot - Anti-RhoA + RhoB (ADP-ribosylated) antibody [VIF140-A1] (<a href='/en-us/products/primary-antibodies/rhoa-rhob-adp-ribosylated-antibody-vif140-a1-ab324028'>ab324028</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc-his-tag whole cell lysate at 20 µg

Lane 2:

293T transfected with an empty vector containing a myc-his-tag were treated with 500nM C3 for 30 minutes whole cell lysate at 20 µg

Lane 3:

293T transfected with an human RhoA expression vector containing a myc-his-tag whole cell lysate at 20 µg

Lane 4:

293T transfected with an human RhoA expression vector containing a myc-his-tag were treated with 500nM C3 for 30 minutes whole cell lysate at 20 µg

Lane 5:

293T transfected with an human RhoB expression vector containing a myc-his-tag whole cell lysate at 20 µg

Lane 6:

293T transfected with an human RhoB expression vector containing a myc-his-tag were treated with 500nM C3 for 30 minutes whole cell lysate at 20 µg

Lane 7:

293T transfected with an human RhoC expression vector containing a myc-his-tag whole cell lysate at 20 µg

Lane 8:

293T transfected with an human RhoC expression vector containing a myc-his-tag were treated with 500nM C3 for 30 minutes whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1/10000 dilution

Observed band size: 22 kDa,17 kDa

false

Exposure time: 1s

• WB

Supplier Data

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (AB98624)

False colour image of Western blot : Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [EPR25038-122] - BSA and Azide free - Human IgG1 (Chimeric) (ab324889) staining at 1/1000 dilution, shown in black.

In Western blot, ab324889 was shown to bind specifically to SARS-CoV-2 Spike Glycoprotein S2. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1h at room temperature, washed again four times then imaged.

Blocking buffer : 3% milk in TBS-0.1% Tween® 20 (TBS-T)

All lanes:

Western blot - Anti-SARS-CoV-2 S Glycoprotein S2 antibody [EPR25038-122] - Human IgG1 (Chimeric) - BSA & Azide free (<a href='/en-us/products/primary-antibodies/sars-cov-2-spike-glycoprotein-s2-antibody-epr25038-122-human-igg1-chimeric-bsa-and-azide-free-ab324889'>ab324889</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera) (<a href='/en-us/products/proteins-peptides/recombinant-human-coronavirus-sars-cov-2-spike-glycoprotein-s2-fc-chimera-ab272106'>ab272106</a>) at 0.2 µg

Lane 2:

Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (<a href='/en-us/products/proteins-peptides/recombinant-human-coronavirus-sars-cov-2-nucleocapsid-protein-his-tag-ab273530'>ab273530</a>) at 0.2 µg

Secondary

All lanes:

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1/10000 dilution

Observed band size: 135 kDa

false

Exposure time: 10s

• Dot

Supplier Data

Dot Blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (AB98624)

Dot blot analysis of bat SARS-like coronavirus RsSHC014 Spike glycoprotein using ab317329 at 1 : 1000 followed by a Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1 : 10,000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-bat SARS-like coronavirus RsSHC014 Spike glycoprotein antibody [abd166] (<a href='/en-us/products/primary-antibodies/bat-sars-like-coronavirus-rsshc014-spike-glycoprotein-antibody-abd166-ab317329'>ab317329</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-his tag whole cell lysate

Lane 2:

293T cells transfected with a Bat Spike glycoprotein expression vector containing a myc-his-tag whole cell lysate

Lane 3:

His-tagged bat SARS-like coronavirus RsSHC01 recombinant fragment protein

Secondary

All lanes:

Dot Blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1/10000 dilution

false

Exposure time: 180s