Name: Goat Anti-Human IgG Fc (HRP) preadsorbed
SKU: ab98624
Rating: 5 (2 reviews)

Goat Anti-Human IgG Fc (HRP) is a preadsorbed secondary antibody for chemiluminescent detection. Ideal for Western blot. Suitable for Western blot, IHC, ELISA and ICC.

- Preadsorbed to prevent cross-reactivity and reduce background staining
- Cited in over 30 publications

Images

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (AB98624), expandable thumbnail

Publications

Key facts

Host species: Goat
Target species: Human
Target isotype: IgG
Target specificity: Fc region
Minimal cross-reactivity: Mouse, Rat, Sheep, Rabbit, Goat, Horse, Chicken, Cow
Conjugation: HRP
Applications: ICC, IHC-P, ELISA, WB
Clonality: Polyclonal

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Reactivity data

Application	Reactivity	Dilution info	Notes
Application ICC	Reactivity Reacts	Dilution info -	Notes -
Application IHC-P	Reactivity Reacts	Dilution info 1/200.00000 - 1/2000.00000	Notes -
Application ELISA	Reactivity Reacts	Dilution info 1/10000.00000 - 1/100000.00000	Notes (Primary).
Application WB	Reactivity Reacts	Dilution info 1/2000.00000 - 1/25000.00000	Notes (Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000).

Target data

See full target information

Function

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). Mediates IgG effector functions on monocytes triggering ADCC of virus-infected cells.

Additional Targets

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Alternative names

Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1

Key facts

Description: Goat Anti-Human IgG Fc (HRP) preadsorbed
Host species: Goat
Target species: Human
Target isotype: IgG
Target specificity: Fc region
Minimal cross-reactivity: Mouse, Rat, Sheep, Rabbit, Goat, Horse, Chicken, Cow
Conjugation: HRP
Applications: ICC, IHC-P, ELISA, WB
Clonality: Polyclonal
Pre-adsorbed: Yes
Isotype: IgG
Concentration

Properties

Form: Liquid
Storage buffer: pH: 6.8 - 7.4
Constituents: PBS, 0.2% BSA, 0.00063% 2-methyl-4-isothiazolin-3-one, 0.00063% 5-chloro-2-methyl-4-isothiazolin-3-one
Purification technique: Affinity purification Immunogen
Purification notes: This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase.

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

This antibody reacts with the Fc region of Human IgG

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9 product images

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624)
This data was developed using Anti-Myc tag antibody [Hyper-myc] ab289981, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concnetration are 5% NFDM/TBST.
Anti-Myc tag antibody [Hyper-myc] ab289981 detects a ranged of myc tagged proteins in WB.
All lanes: Western blot - Anti-Myc tag antibody [Hyper-myc] (Anti-Myc tag antibody [Hyper-myc] ab289981) at 1/5000 dilution
Lane 1: HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 1 µg
Lane 2: HEK-293T transfected with cas9 ( S. Aureas) expression vector containing a myc-His-tag®, whole cell lysate at 1 µg
Lane 3: HEK-293T transfected with human SNCA140 expression vector containing a myc-His-tag®, whole cell lysate at 1 µg
Lane 4: HEK-293T transfected with human IDS expression vector containing a myc-His-tag®, whole cell lysate at 1 µg
Lane 5: HEK-293T transfected with human NF2 S518A expression vector containing a myc-His-tag®, whole cell lysate at 1 µg
Lane 6: HEK-293T transfected with human Alpha-synuclein expression vector containing a myc-His-tag®, whole cell lysate at 1 µg
Secondary
All lanes: Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1/10000 dilution
Predicted band size: 49 kDa
Exposure time: 1s
Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624)
Blocking and diluting buffer was 5% NFDM/TBST
All lanes: Western blot - Anti-PLBD2 antibody [IGX4128H] (Anti-PLBD2 antibody [IGX4128H] ab213295) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse heart tissue lysate at 20 µg
Lane 3: Human cerebellum tissue lysate at 20 µg
Lane 4: Rat brain tissue lysate at 20 µg
Lane 5: Rat heart tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1/10000 dilution
Exposure time: 4s
Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624)
Lane 1: Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active, His tagged) (Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) ab273068) at 0.2 ug
Lane 2: Recombinant SARS-COV-2 NSP2 protein (His tagged) at 0.2 ug
Performed under reducing conditions.
Observed band size: 135 kDa.
False colour image of Western blot: Rabbit monoclonal [EPR24852-116] to SARS-CoV-2 Spike Glycoprotein S1 - Human IgG1 (Chimeric), Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [EPR24852-116] - Human IgG1 (Chimeric) ab323000 staining at 1/500 dilution, shown in black; Mouse Monoclonal 6X His tag® (Anti-6X His tag® antibody [HIS.H8] ab18184) loading control staining at 1/1000 dilution, shown in red. In Western blot, Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [EPR24852-116] - Human IgG1 (Chimeric) ab323000 was shown to bind specifically to SARS-CoV-2 spike glycoprotein S1. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1:10000 and Goat anti-Mouse IgG H&L 680RD at 1:20000.
Blocking buffer: 3% milk in TBS-0.1% Tween® 20 (TBS-T).
Exposure time: 30 Sec.
All lanes: Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [EPR24852-116] - Human IgG1 (Chimeric) (Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [EPR24852-116] - Human IgG1 (Chimeric) ab323000) at 1/500 dilution
Lane 1: Western blot - Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) (Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) ab273068) at 0.2 µg
Lane 2: Recombinant SARS-COV-2 NSP2 protein (His tagged) at 0.2 µg
Secondary
Lanes 1 - 2: Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1/10000 dilution
Lanes 1 - 2: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 135 kDa
Exposure time: 30s
Indirect ELISA - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624)
Indirect ELISA showing primary antibody Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [EPR24852-116] - Human IgG1 (Chimeric) ab323000 binding to Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera) ab272105). Plates were coated with Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera) ab272105), Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera, Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera) ab272106) and Recombinant human coronavirus SARS spike glycoprotein (Tagged, Recombinant Human coronavirus SARS spike glycoprotein (Tagged) ab270844) at 1000 ng/ml. Binding of Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [EPR24852-116] - Human IgG1 (Chimeric) ab323000 was assessed in a serial dilution range 0.016- 1000 ng/mL (a 3-fold serial dilution). Secondary antibody, Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) was used at 1:5000.
Indirect ELISA - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624)
Indirect ELISA showing primary antibody Anti-SARS-CoV-2 Spike Glycoprotein S1 [8B12-C2] – Human IgG1 (Chimeric) ab322272 binding to Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera) ab272105). Plates were coated with Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera) ab272105) and Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera, Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera) ab272106) at 1000 ng/ml. Binding of Anti-SARS-CoV-2 Spike Glycoprotein S1 [8B12-C2] – Human IgG1 (Chimeric) ab322272 was assessed in a serial dilution range 0.016- 1000 ng/mL (a 3-fold serial dilution). Secondary antibody, Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) was used at 1:5000.
Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624)
False colour image of Western blot: Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] - Human IgG1 (Chimeric) (Anti-SARS-CoV-2 Spike Glycoprotein S1 [8B12-C2] – Human IgG1 (Chimeric) ab322272) staining at 1/500 dilution, shown in black; Mouse Monoclonal 6X His tag® (Anti-6X His tag® antibody [HIS.H8] ab18184) loading control staining at 1/200 dilution, shown in red. In Western blot, Anti-SARS-CoV-2 Spike Glycoprotein S1 [8B12-C2] – Human IgG1 (Chimeric) ab322272 was shown to bind specifically to SARS-CoV and SARS-CoV-2 Spike Glycoproteins. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1:10000 and Goat anti-Mouse IgG H&L 680RD at 1:20000.
All lanes: Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 [8B12-C2] – Human IgG1 (Chimeric) (Anti-SARS-CoV-2 Spike Glycoprotein S1 [8B12-C2] – Human IgG1 (Chimeric) ab322272) at 1/500 dilution
Lane 1: Western blot - Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) (Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) ab273068) at 0.2 µg
Lane 2: SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His Recombinant Protein at 0.2 µg
Lane 3: SARS-CoV Spike S1+S2 ECD-His Recombinant Protein at 0.2 µg
Secondary
Lanes 1 - 3: Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1/10000 dilution
Lanes 1 - 3: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 120-160 kDa
Exposure time: 5s
Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624)
All lanes: Western blot - Anti-HMGB1 antibody [EPR21207] (Anti-HMGB1 antibody [EPR21207] ab213256) at 1/100 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 5: parental HAP1(HOWT01) (human chronic myelogenous leukemia near-haploid cell ) whole cell lysate at 20 µg
Lane 6: HMGB1 knockout HAP1 whole cell lysate at 20 µg
Lane 7: MEF (mouse embryo fibroblast) whole cell lysate at 20 µg
Lane 8: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 9: Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1/10000 dilution
Dot Blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624)
Dot blot analysis of bat SARS-like coronavirus RsSHC014 Spike glycoprotein using Anti-bat SARS-like coronavirus RsSHC014 Spike glycoprotein antibody [abd166] ab317329 at 1:1000 followed by a Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1:10,000 dilution.
All lanes: Dot Blot - Anti-bat SARS-like coronavirus RsSHC014 Spike glycoprotein antibody [abd166] (Anti-bat SARS-like coronavirus RsSHC014 Spike glycoprotein antibody [abd166] ab317329) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containing a myc-his tag whole cell lysate
Lane 2: 293T cells transfected with a Bat Spike glycoprotein expression vector containing a myc-his-tag whole cell lysate
Lane 3: His-tagged bat SARS-like coronavirus RsSHC01 recombinant fragment protein
Secondary
All lanes: Dot Blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1/10000 dilution
Exposure time: 180s
Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624)
Blocking buffer and concentration: 5% NFDM/TBST

Diluting buffer and concentration: 5% NFDM/TBST

Exposure Time: Lane 1: 3 seconds, Lane 2-3: 20 seconds.

Negative control: Skeletal muscle.

The identity of the bands between 27 kDa and 150 kDa are unknown.

Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a loading control.
All lanes: Western blot - Anti-TAGLN/Transgelin antibody [EPR21206] (Anti-TAGLN/Transgelin antibody [EPR21206] ab213273) at 1/500 dilution
Lane 1: Rat bladder tissue lysate at 20 µg
Lane 2: Rat colon tissue lysate at 20 µg
Lane 3: Rat skeletal muscle tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1/10000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa