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Reactivity data

Application

EM

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).

Alternative names

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Suitable for EM.

Alternative names

Key facts

Description

Goat Anti-Human IgG H&L (6nm Gold)

Host species

Goat

Target species

Human

Target isotype

IgG

Target specificity

Heavy & Light chains

Conjugation

6nm Gold

Applications

EM

Clonality

Polyclonal

Pre-adsorbed

No

Isotype

IgG

Concentration
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Properties

Form

Liquid

Storage buffer

Preservative: 0.097% Sodium azide
Constituents: PBS, 1% BSA

Purification technique

Affinity purification Immunogen

Purification notes

This product has been immuno-affinity purified and immuno cross-absorbed to reduce non-specific reactions.The activity of each lot is determined using a dot-spot test system as described by Moeremans et al., J. Immunol. Methods, 74, (1984), 353.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

This product is an immunogold reagent built around colloidal gold particles of 6nm. The paritcle population is monodisperse and thus shows minimal size variation and overlap. Typically, the coefficient of variance for the 6nm particle size conjugate is less than 12 %.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com

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