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Suitable for WB, Dot, ELISA, IHC-Fr, IHC-P, IM, ICC/IF. Cited in 1 publication.
Alternative names=Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1
Goat
Human
IgG
Heavy & Light chains
Biotin
WB, Dot, ELISA, IHC-Fr, IHC-P, IM, ICC/IF
Polyclonal
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application Dot | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application IM | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/1000 - 1/5000 | Notes - |
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).
Suitable for WB, Dot, ELISA, IHC-Fr, IHC-P, IM, ICC/IF. Cited in 1 publication.
Alternative names=Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1
Goat Anti-Human IgG H&L (Biotin)
Goat
Human
IgG
Heavy & Light chains
Biotin
WB, Dot, ELISA, IHC-Fr, IHC-P, IM, ICC/IF
Polyclonal
No
Liquid
pH: 6.5
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.88% Sodium chloride, 0.42% Tripotassium orthophosphate
Affinity purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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