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Suitable for WB, Dot, ELISA, IHC-Fr, IHC-P, IM, ICC/IF. Cited in 1 publication.

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Host species

Goat

Target species

Human

Target isotype

IgG

Target specificity

Heavy & Light chains

Conjugation

Biotin

Applications

WB, Dot, ELISA, IHC-Fr, IHC-P, IM, ICC/IF

Clonality

Polyclonal

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Reactivity data

Application
WB
Reactivity
Reacts
Dilution info
-
Notes

-

Application
Dot
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC-Fr
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC-P
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IM
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ICC/IF
Reactivity
Reacts
Dilution info
1/1000 - 1/5000
Notes

-

Target data

Function

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).

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Suitable for WB, Dot, ELISA, IHC-Fr, IHC-P, IM, ICC/IF. Cited in 1 publication.

Key facts

Description

Goat Anti-Human IgG H&L (Biotin)

Host species

Goat

Target species

Human

Target isotype

IgG

Target specificity

Heavy & Light chains

Conjugation

Biotin

Applications

WB, Dot, ELISA, IHC-Fr, IHC-P, IM, ICC/IF

Clonality

Polyclonal

Pre-adsorbed

No

Concentration
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Properties

Form

Liquid

Storage buffer

pH: 6.5
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.88% Sodium chloride, 0.42% Tripotassium orthophosphate

Purification technique

Affinity purification

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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