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Reactivity data

Application

ICC/IF

Reactivity

Reacts

Dilution info

1/50.00000 - 1/500.00000

Notes

-

Application

Flow Cyt

Reactivity

Reacts

Dilution info

1/50.00000 - 1/200.00000

Notes

-

Application

IHC-P

Reactivity

Reacts

Dilution info

1/50.00000 - 1/500.00000

Notes

DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments.

Target data

Function

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).

Alternative names

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Suitable for ICC/IF, Flow Cyt, IHC-P. Cited in 1 publication.

Alternative names

Key facts

Description

Goat Anti-Human IgG H&L (DyLight® 650)

Host species

Goat

Target species

Human

Target isotype

IgG

Target specificity

Heavy & Light chains

Conjugation

DyLight® 650

Applications

ICC/IF, Flow Cyt, IHC-P

Clonality

Polyclonal

Pre-adsorbed

No

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Human IgG and with light chains common to other Human immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

Isotype

IgG

Concentration
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Properties

Form

Liquid

Storage buffer

pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

DyLight® 650 replaces DyLight® 649.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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