Skip to main content

Suitable for Dot, ELISA, IHC-Fr, IHC-P, IM, WB, ICC/IF. Ideal for western blot. Cited in 47 publications.

Be the first to review this product! Submit a review

Images

Key facts

Host species

Goat

Target species

Human

Target isotype

IgG

Target specificity

Heavy & Light chains

Conjugation

HRP

Applications

Dot, ELISA, IHC-Fr, IHC-P, IM, WB, ICC/IF

Clonality

Polyclonal

Abcam Recommends

Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Reactivity data

Application

Dot

Reactivity

Reacts

Dilution info

-

Notes

-

Application

ELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Application

IHC-Fr

Reactivity

Reacts

Dilution info

-

Notes

-

Application

IHC-P

Reactivity

Reacts

Dilution info

-

Notes

-

Application

IM

Reactivity

Reacts

Dilution info

-

Notes

-

Application

WB

Reactivity

Reacts

Dilution info

1/5000 - 1/25000

Notes

-

Application

ICC/IF

Reactivity

Reacts

Dilution info

1/1000 - 1/5000

Notes

-

Target data

Function

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).

Alternative names

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Suitable for Dot, ELISA, IHC-Fr, IHC-P, IM, WB, ICC/IF. Ideal for western blot. Cited in 47 publications.

Alternative names

Key facts

Description

Goat Anti-Human IgG H&L (HRP)

Host species

Goat

Target species

Human

Target isotype

IgG

Target specificity

Heavy & Light chains

Conjugation

HRP

Applications

Dot, ELISA, IHC-Fr, IHC-P, IM, WB, ICC/IF

Clonality

Polyclonal

Pre-adsorbed

No

Isotype

IgG

Concentration
Loading...

Properties

Form

Liquid

Storage buffer

pH: 6.5
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate

Purification technique

Affinity purification

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

  • Dot Blot - Goat Anti-Human IgG H&L (HRP) (ab6858), expandable thumbnail

    Dot Blot - Goat Anti-Human IgG H&L (HRP) (ab6858)

    Dot blot analysis of human IgG using ab6858 at 1/1000 dilution.

    The indicated qualtities of human IgG were spotted. Blocking was performed for 1 hour at room temperature using a proprietary blocking buffer prior to addition of ab6858.

  • Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858), expandable thumbnail

    Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858)

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-MERS-Cov Nucleocapsid protein antibody [abd211] (AB316332) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) conteining a myc-His-tag®, whole cell lysate at 20 µg

    Lane 2: 293T transfected with a MERS-CoV Nucleocapsid Protein expression vector conteining a myc-His-tag®, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Human IgG H&L (HRP) (AB6858) at 1/5000 dilution

    Observed band size: 46 kDa, 36 kDa

    Exposure time: 1s

  • Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858), expandable thumbnail

    Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858)

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-MERS-Cov Nucleocapsid protein antibody [abd211] (AB316332) at 1/1000 dilution

    All lanes: His-tagged MERS-CoV Nucleocapsid recombinant protein at 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Human IgG H&L (HRP) (AB6858) at 1/5000 dilution

    Observed band size: 46 kDa

    Exposure time: 180s

  • Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858), expandable thumbnail

    Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858)

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-MERS-CoV Nucleocapsid protein antibody [abd208] (AB316330) at 1/1000 dilution

    All lanes: His-tagged MERS-CoV Nucleocapsid recombinant protein at 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Human IgG H&L (HRP) (AB6858) at 1/5000 dilution

    Developed using the ECL technique.

    Observed band size: 46 kDa

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Human IgG H&L (HRP) (ab6858), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Human IgG H&L (HRP) (ab6858)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue sections labelling TAGLN/Transgelin at 1/100 dilution (5 μg/ml) followed by Goat Anti-Human IgG H&L (HRP) (ab6858) secondary antibody at 1/1000 dilution. Negative control: no staining in rat skeletal muscle. The section was incubated with ab213273 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Human IgG H&L (HRP) (ab6858), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Human IgG H&L (HRP) (ab6858)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue sections labelling TAGLN/Transgelin at 1/100 dilution (5 μg/ml) followed by Goat Anti-Human IgG H&L (HRP) (ab6858) secondary antibody at 1/1000 dilution. Positive staining on the smooth muscle in rat lung. The section was incubated with ab213273 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Human IgG H&L (HRP) (ab6858), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Human IgG H&L (HRP) (ab6858)

    Immunohistochemical analysis of paraffin-embedded rat esophagus tissue sections labelling TAGLN/Transgelin at 1/100 dilution (5 μg/ml) followed by Goat Anti-Human IgG H&L (HRP) (ab6858) secondary antibody at 1/1000 dilution. Positive staining on the smooth muscle in rat esophagus. The section was incubated with ab213273 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

  • Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858), expandable thumbnail

    Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) was used at 1:5000 dilution.

    All lanes: Western blot - Anti-HCoV-OC43 Nucleoprotein antibody [abd384] (AB309964) at 1/1000 dilution

    All lanes: His-tagged HCoV-OC43 Nucleoprotein recombinant protein at 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Human IgG H&L (HRP) (AB6858) at 1/5000 dilution

    Observed band size: 65 kDa

    Exposure time: 3min

  • Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858), expandable thumbnail

    Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) was used at 1:200000 dilution.
    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) was used at 1:5000 dilution.

    All lanes: Western blot - Anti-HCoV-OC43 Nucleoprotein antibody [abd384] (AB309964) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cells) transfected with an empty vector containing a His-tag, whole cell lysate at 4 µg

    Lane 2: 293T (human embryonic kidney epithelial cells) transfected with HCoV-OC43 Nucleoprotein expression vector containing a His-tag, whole cell lysate at 4 µg

    Secondary

    All lanes: Western blot - Goat Anti-Human IgG H&L (HRP) (AB6858) at 1/5000 dilution

    Observed band size: 65 kDa

    Exposure time: 1s

  • Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858), expandable thumbnail

    Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) was used at 1:5000 dilution.

    All lanes: Western blot - Anti-HCoV-OC43 Nucleoprotein antibody [abd385] (AB309966) at 1/1000 dilution

    All lanes: His-tagged HCoV-OC43 Nucleoprotein recombinant protein at 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Human IgG H&L (HRP) (AB6858) at 1/5000 dilution

    Observed band size: 65 kDa

    Exposure time: 3min

  • Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858), expandable thumbnail

    Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858)

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-NL63 Nucleoprotein antibody [abd259] (AB316212) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) conteining a myc-His-tag®, whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with an human coronavirus NL63 Nucleoprotein expression vector conteining a myc-His-tag®, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Human IgG H&L (HRP) (AB6858) at 1/5000 dilution

    Observed band size: 50 kDa, 36 kDa

    Exposure time: 1s

  • Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858), expandable thumbnail

    Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) was used at 1:200000 dilution.
    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) was used at 1:5000 dilution.

    All lanes: Western blot - Anti-HCoV-OC43 Nucleoprotein antibody [abd385] (AB309966) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cells) transfected with an empty vector containing a His-tag, whole cell lysate at 4 µg

    Lane 2: 293T (human embryonic kidney epithelial cells) transfected with HCoV-OC43 Nucleoprotein expression vector containing a His-tag, whole cell lysate at 4 µg

    Secondary

    All lanes: Western blot - Goat Anti-Human IgG H&L (HRP) (AB6858) at 1/5000 dilution

    Observed band size: 65 kDa

    Exposure time: 3.25s

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com