Goat Anti-Human IgG H&L (HRP)

• Dot

Supplier Data

Dot Blot - Goat Anti-Human IgG H&L (HRP) (AB6858)

Dot blot analysis of human IgG using ab6858 at 1/1000 dilution.

The indicated qualtities of human IgG were spotted. Blocking was performed for 1 hour at room temperature using a proprietary blocking buffer prior to addition of ab6858.

• ELISA

Supplier Data

ELISA - Goat Anti-Human IgG H&L (HRP) (AB6858)

ELISA results of ab6858 against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 1.0 µg of Human IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Human IgG H&L (HRP) (AB6858)

Immunohistochemical analysis of paraffin-embedded rat esophagus tissue sections labelling TAGLN/Transgelin at 1/100 dilution (5 μg/ml) followed by Goat Anti-Human IgG H&L (HRP) (ab6858) secondary antibody at 1/1000 dilution. Positive staining on the smooth muscle in rat esophagus. The section was incubated with ab213273 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Human IgG H&L (HRP) (AB6858)

Immunohistochemical analysis of paraffin-embedded rat lung tissue sections labelling TAGLN/Transgelin at 1/100 dilution (5 μg/ml) followed by Goat Anti-Human IgG H&L (HRP) (ab6858) secondary antibody at 1/1000 dilution. Positive staining on the smooth muscle in rat lung. The section was incubated with ab213273 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Human IgG H&L (HRP) (AB6858)

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue sections labelling TAGLN/Transgelin at 1/100 dilution (5 μg/ml) followed by Goat Anti-Human IgG H&L (HRP) (ab6858) secondary antibody at 1/1000 dilution. Negative control : no staining in rat skeletal muscle. The section was incubated with ab213273 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

• WB

Supplier Data

Western blot - Goat Anti-Human IgG H&L (HRP) (AB6858)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-NL63 Nucleoprotein antibody [abd259] (<a href='/en-us/products/primary-antibodies/nl63-nucleoprotein-antibody-abd259-ab316212'>ab316212</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) conteining a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with an human coronavirus NL63 Nucleoprotein expression vector conteining a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858) at 1/5000 dilution

Observed band size: 50 kDa,36 kDa

false

Exposure time: 1s

• WB

Supplier Data

Western blot - Goat Anti-Human IgG H&L (HRP) (AB6858)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody [S5V2-29] (<a href='/en-us/products/primary-antibodies/influenza-a-virus-h3n2-a-texas-50-2012-hemagglutinin-antibody-s5v2-29-ab322519'>ab322519</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T transfected with a Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

293T transfected with a Influenza A Virus H1N1 (A/California/04/2009) Hemagglutinin expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 4:

293T transfected with a Influenza A Virus H5N1 (strain A/Vietnam/1203/2004 H5N1) Hemagglutinin expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858) at 1/10000 dilution

Observed band size: 75 kDa,36 kDa

false

Exposure time: 4s

• WB

Supplier Data

Western blot - Goat Anti-Human IgG H&L (HRP) (AB6858)

Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) was used at 1 : 5000 dilution.

All lanes:

Western blot - Anti-HCoV-OC43 Nucleoprotein antibody [abd384] (<a href='/en-us/products/primary-antibodies/hcov-oc43-nucleoprotein-antibody-abd384-ab309964'>ab309964</a>) at 1/1000 dilution

All lanes:

His-tagged HCoV-OC43 Nucleoprotein recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858) at 1/5000 dilution

Observed band size: 65 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Goat Anti-Human IgG H&L (HRP) (AB6858)

Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) was used at 1 : 5000 dilution.

All lanes:

Western blot - Anti-HCoV-OC43 Nucleoprotein antibody [abd385] (<a href='/en-us/products/primary-antibodies/hcov-oc43-nucleoprotein-antibody-abd385-ab309966'>ab309966</a>) at 1/1000 dilution

All lanes:

His-tagged HCoV-OC43 Nucleoprotein recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858) at 1/5000 dilution

Observed band size: 65 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Goat Anti-Human IgG H&L (HRP) (AB6858)

Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) was used at 1 : 200000 dilution. In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) was used at 1 : 5000 dilution.

All lanes:

Western blot - Anti-HCoV-OC43 Nucleoprotein antibody [abd385] (<a href='/en-us/products/primary-antibodies/hcov-oc43-nucleoprotein-antibody-abd385-ab309966'>ab309966</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cells) transfected with an empty vector containing a His-tag, whole cell lysate at 4 µg

Lane 2:

293T (human embryonic kidney epithelial cells) transfected with HCoV-OC43 Nucleoprotein expression vector containing a His-tag, whole cell lysate at 4 µg

Secondary

All lanes:

Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858) at 1/5000 dilution

Observed band size: 65 kDa

false

Exposure time: 3.25s

• WB

Supplier Data

Western blot - Goat Anti-Human IgG H&L (HRP) (AB6858)

Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) was used at 1 : 200000 dilution. In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) was used at 1 : 5000 dilution.

All lanes:

Western blot - Anti-HCoV-OC43 Nucleoprotein antibody [abd384] (<a href='/en-us/products/primary-antibodies/hcov-oc43-nucleoprotein-antibody-abd384-ab309964'>ab309964</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cells) transfected with an empty vector containing a His-tag, whole cell lysate at 4 µg

Lane 2:

293T (human embryonic kidney epithelial cells) transfected with HCoV-OC43 Nucleoprotein expression vector containing a His-tag, whole cell lysate at 4 µg

Secondary

All lanes:

Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858) at 1/5000 dilution

Observed band size: 65 kDa

false

Exposure time: 1s

• WB

Supplier Data

Western blot - Goat Anti-Human IgG H&L (HRP) (AB6858)

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-MERS-Cov Nucleocapsid protein antibody [abd211] (<a href='/en-us/products/primary-antibodies/mers-cov-nucleocapsid-protein-antibody-abd211-ab316332'>ab316332</a>) at 1/1000 dilution

All lanes:

His-tagged MERS-CoV Nucleocapsid recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858) at 1/5000 dilution

Observed band size: 46 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Goat Anti-Human IgG H&L (HRP) (AB6858)

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-MERS-CoV Nucleocapsid protein antibody [abd208] (<a href='/en-us/products/primary-antibodies/mers-cov-nucleocapsid-protein-antibody-abd208-ab316330'>ab316330</a>) at 1/1000 dilution

All lanes:

His-tagged MERS-CoV Nucleocapsid recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858) at 1/5000 dilution

Observed band size: 46 kDa

true

Exposure time: 180s

• WB

Supplier Data

Western blot - Goat Anti-Human IgG H&L (HRP) (AB6858)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-MERS-Cov Nucleocapsid protein antibody [abd211] (<a href='/en-us/products/primary-antibodies/mers-cov-nucleocapsid-protein-antibody-abd211-ab316332'>ab316332</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) conteining a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T transfected with a MERS-CoV Nucleocapsid Protein expression vector conteining a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858) at 1/5000 dilution

Observed band size: 46 kDa,36 kDa

false

Exposure time: 1s