Goat anti-human IgG H&L (HRP) is a secondary antibody for chemiluminescent detection. Ideal for western blot. Suitable for ICC, IHC, western blot, dot blot and ELISA.
- Validated for immunostaining on a Leica Biosystems BOND® RX instrument
- Cited in over 40 publications
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- Heliyon 10:e234782024Comparison of neutralization potency across passive immunotherapy approaches as potential treatments for emerging infectious diseases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHossein Ranjbaran,Yahya Ehteshaminia,Mohammadreza Nadernezhad,Seyedeh Farzaneh Jalali,Farhad Jadidi-Niaragh,Abdol Sattar Pagheh,Seyed Ehsan Enderami,Saeid Abedian Kenari,Hadi HassanniaPubMed 38226283
- Nature cell biology 25:1223-12342023A placental model of SARS-CoV-2 infection reveals ACE2-dependent susceptibility and differentiation impairment in syncytiotrophoblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJ Chen,J A Neil,J P Tan,R Rudraraju,M Mohenska,Y B Y Sun,E Walters,N G Bediaga,G Sun,Y Zhou,Y Li,D Drew,P Pymm,W H Tham,Y Wang,F J Rossello,G Nie,X Liu,K Subbarao,J M PoloPubMed 37443288
- Cancer research communications 3:640-6582023Identification of Membrane-expressed CAPRIN-1 as a Novel and Universal Cancer Target, and Generation of a Therapeutic Anti-CAPRIN-1 Antibody TRK-950.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFumiyoshi Okano,Takanori Saito,Yoshitaka Minamida,Shinichi Kobayashi,Takayoshi Ido,Yasushi Miyauchi,Ukei Wasai,Daisuke Akazawa,Masahiko Kume,Masaki Ishibashi,Ke Jiang,Alexandra Aicher,Christopher Heeschen,Tetsu YoneharaPubMed 37082579
- Acta biochimica et biophysica Sinica 54:1909-19162023Screening of autoantibodies as biomarkers in the serum of renal cancer patients based on human proteome microarray.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYangyang Sun,Chengxi Liu,Huidong Zhong,Chenguang Wang,Haibo Xu,Wei ChenPubMed 36789694
- Microorganisms 11:2023Cross-Reactivity of Antibodies in Intravenous Immunoglobulin Preparation for Protection against SARS-CoV-2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesToshifumi Osaka,Yoko Yamamoto,Takehisa Soma,Naoko Yanagisawa,Satoru NagataPubMed 36838436
- Experimental and therapeutic medicine 25:292022Altered genome‑wide hydroxymethylation analysis for neoadjuvant chemoradiotherapy followed by surgery in esophageal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXianjing Zhang,Mingzhu Lu,Jing Zhu,Changsong Zhang,Meihua WangPubMed 36561617
- Journal of extracellular vesicles 11:e122472022Generalized Modules for Membrane Antigens (GMMA), an outer membrane vesicle-based vaccine platform, for efficient viral antigen delivery.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai Hu,Elena Palmieri,Karnyart Samnuan,Beatrice Ricchetti,Davide Oldrini,Paul F McKay,Guanghui Wu,Leigh Thorne,Anthony R Fooks,Lorraine M McElhinney,Hooman Goharriz,Megan Golding,Robin J Shattock,Francesca MicoliPubMed 36377074
- Nature protocols 18:530-5542022A bifunctional molecule-assisted synthesis of mimics for use in probing the ubiquitination system.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingyun Zheng,Tian Wang,Junxiong Mao,Guochao Chu,Lujun Liang,Yangwode Jing,Chong Zuo,Yuanyuan Yu,Honggang Hu,Man PanPubMed 36323865
- PLoS pathogens 18:e10107542022Localization and functions of native and eGFP-tagged capsid proteins in HIV-1 particles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAshwanth C Francis,Anna Cereseto,Parmit K Singh,Jiong Shi,Eric Poeschla,Alan N Engelman,Christopher Aiken,Gregory B MelikyanPubMed 35951676
- Cell reports 39:1109052022Ultrapotent and broad neutralization of SARS-CoV-2 variants by modular, tetravalent, bi-paratopic antibodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShane Miersch,Nitin Sharma,Reza Saberianfar,Chao Chen,Francesca Caccuri,Alberto Zani,Arnaldo Caruso,James Brett Case,Michael S Diamond,Gaya K Amarasinghe,Giuseppe Novelli,Sachdev S SidhuPubMed 35617963
Key facts
- Host species
- Goat
- Target species
- Human
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- HRP
- Applications
- Dot, ELISA, IHC-Fr, IHC-P, IM, WB, ICC/IF
- Clonality
- Polyclonal
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Dot | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Expected | Dilution info - | Notes - |
Application IHC-P | Reactivity Expected | Dilution info - | Notes - |
Application IM | Reactivity Expected | Dilution info - | Notes - |
Application WB | Reactivity Expected | Dilution info 1/5000 - 1/25000 | Notes - |
Application ICC/IF | Reactivity Expected | Dilution info 1/1000 - 1/5000 | Notes - |
Target data
Function
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). Mediates IgG effector functions on monocytes triggering ADCC of virus-infected cells.
Additional Targets
, ,
Alternative names
Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1
Recommended products
Goat anti-human IgG H&L (HRP) is a secondary antibody for chemiluminescent detection. Ideal for western blot. Suitable for ICC, IHC, western blot, dot blot and ELISA.
- Validated for immunostaining on a Leica Biosystems BOND® RX instrument
- Cited in over 40 publications
Key facts
- Description
- Goat Anti-Human IgG H&L (HRP)
- Host species
- Goat
- Target species
- Human
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- HRP
- Applications
- Dot, ELISA, IHC-Fr, IHC-P, IM, WB, ICC/IF
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Purification technique
- Affinity purification
- Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies , designed to deliver precise and reliable results.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
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Full details and terms and conditions can be found here:
Terms & Conditions.
14 product images
Dot Blot - Goat Anti-Human IgG H&L (HRP) (ab6858)
Dot blot analysis of human IgG using ab6858 at 1/1000 dilution.
The indicated qualtities of human IgG were spotted. Blocking was performed for 1 hour at room temperature using a proprietary blocking buffer prior to addition of ab6858.
ELISA - Goat Anti-Human IgG H&L (HRP) (ab6858)
ELISA results of ab6858 against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 1.0 µg of Human IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.
Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody [S5V2-29] (Anti-Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin antibody [S5V2-29] ab322519) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 2: 293T transfected with a Influenza A Virus H3N2 (A/Texas/50/2012) Hemagglutinin expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 3: 293T transfected with a Influenza A Virus H1N1 (A/California/04/2009) Hemagglutinin expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 4: 293T transfected with a Influenza A Virus H5N1 (strain A/Vietnam/1203/2004 H5N1) Hemagglutinin expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858) at 1/10000 dilution
Observed band size: 75 kDa, 36 kDa
Exposure time: 4s
Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858)
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-NL63 Nucleoprotein antibody [abd259] (Anti-NL63 Nucleoprotein antibody [abd259] ab316212) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) conteining a myc-His-tag®, whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: 293T cells transfected with an human coronavirus NL63 Nucleoprotein expression vector conteining a myc-His-tag®, whole cell lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858) at 1/5000 dilution
Observed band size: 50 kDa, 36 kDa
Exposure time: 1s
Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858)
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-MERS-Cov Nucleocapsid protein antibody [abd211] (Anti-MERS-Cov Nucleocapsid protein antibody [abd211] ab316332) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) conteining a myc-His-tag®, whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: 293T transfected with a MERS-CoV Nucleocapsid Protein expression vector conteining a myc-His-tag®, whole cell lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858) at 1/5000 dilution
Observed band size: 46 kDa, 36 kDa
Exposure time: 1s
Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858)
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-MERS-Cov Nucleocapsid protein antibody [abd211] (Anti-MERS-Cov Nucleocapsid protein antibody [abd211] ab316332) at 1/1000 dilution
All lanes: His-tagged MERS-CoV Nucleocapsid recombinant protein at 10 ng with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858) at 1/5000 dilution
Observed band size: 46 kDa
Exposure time: 180s
Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858)
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-MERS-CoV Nucleocapsid protein antibody [abd208] (Anti-MERS-CoV Nucleocapsid protein antibody [abd208] ab316330) at 1/1000 dilution
All lanes: His-tagged MERS-CoV Nucleocapsid recombinant protein at 10 ng with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858) at 1/5000 dilution
Developed using the ECL technique.
Observed band size: 46 kDa
Exposure time: 180s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Human IgG H&L (HRP) (ab6858)
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue sections labelling TAGLN/Transgelin at 1/100 dilution (5 μg/ml) followed by Goat Anti-Human IgG H&L (HRP) (ab6858) secondary antibody at 1/1000 dilution. Negative control: no staining in rat skeletal muscle. The section was incubated with Anti-TAGLN/Transgelin antibody [EPR21206] ab213273 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Human IgG H&L (HRP) (ab6858)
Immunohistochemical analysis of paraffin-embedded rat lung tissue sections labelling TAGLN/Transgelin at 1/100 dilution (5 μg/ml) followed by Goat Anti-Human IgG H&L (HRP) (ab6858) secondary antibody at 1/1000 dilution. Positive staining on the smooth muscle in rat lung. The section was incubated with Anti-TAGLN/Transgelin antibody [EPR21206] ab213273 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Human IgG H&L (HRP) (ab6858)
Immunohistochemical analysis of paraffin-embedded rat esophagus tissue sections labelling TAGLN/Transgelin at 1/100 dilution (5 μg/ml) followed by Goat Anti-Human IgG H&L (HRP) (ab6858) secondary antibody at 1/1000 dilution. Positive staining on the smooth muscle in rat esophagus. The section was incubated with Anti-TAGLN/Transgelin antibody [EPR21206] ab213273 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) was used at 1:5000 dilution.All lanes: Western blot - Anti-HCoV-OC43 Nucleoprotein antibody [abd384] (Anti-HCoV-OC43 Nucleoprotein antibody [abd384] ab309964) at 1/1000 dilution
All lanes: His-tagged HCoV-OC43 Nucleoprotein recombinant protein at 10 ng
SecondaryAll lanes: Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858) at 1/5000 dilution
Observed band size: 65 kDa
Exposure time: 3min
Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) was used at 1:200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) was used at 1:5000 dilution.All lanes: Western blot - Anti-HCoV-OC43 Nucleoprotein antibody [abd384] (Anti-HCoV-OC43 Nucleoprotein antibody [abd384] ab309964) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cells) transfected with an empty vector containing a His-tag, whole cell lysate at 4 µg
Lane 2: 293T (human embryonic kidney epithelial cells) transfected with HCoV-OC43 Nucleoprotein expression vector containing a His-tag, whole cell lysate at 4 µg
SecondaryAll lanes: Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858) at 1/5000 dilution
Observed band size: 65 kDa
Exposure time: 1s
Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) was used at 1:5000 dilution.All lanes: Western blot - Anti-HCoV-OC43 Nucleoprotein antibody [abd385] (Anti-HCoV-OC43 Nucleoprotein antibody [abd385] ab309966) at 1/1000 dilution
All lanes: His-tagged HCoV-OC43 Nucleoprotein recombinant protein at 10 ng
SecondaryAll lanes: Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858) at 1/5000 dilution
Observed band size: 65 kDa
Exposure time: 3min
Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) was used at 1:200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) was used at 1:5000 dilution.All lanes: Western blot - Anti-HCoV-OC43 Nucleoprotein antibody [abd385] (Anti-HCoV-OC43 Nucleoprotein antibody [abd385] ab309966) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cells) transfected with an empty vector containing a His-tag, whole cell lysate at 4 µg
Lane 2: 293T (human embryonic kidney epithelial cells) transfected with HCoV-OC43 Nucleoprotein expression vector containing a His-tag, whole cell lysate at 4 µg
SecondaryAll lanes: Western blot - Goat Anti-Human IgG H&L (HRP) (ab6858) at 1/5000 dilution
Observed band size: 65 kDa
Exposure time: 3.25s
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