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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Suitable for Dot, ELISA, IHC-Fr, IHC-P, IM, WB, ICC/IF. Ideal for western blot. Cited in 47 publications.
Goat
Human
IgG
Heavy & Light chains
HRP
Dot, ELISA, IHC-Fr, IHC-P, IM, WB, ICC/IF
Polyclonal
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application Dot | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application IM | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/5000 - 1/25000 | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/1000 - 1/5000 | Notes - |
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).
Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1
Suitable for Dot, ELISA, IHC-Fr, IHC-P, IM, WB, ICC/IF. Ideal for western blot. Cited in 47 publications.
Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1
Goat Anti-Human IgG H&L (HRP)
Goat
Human
IgG
Heavy & Light chains
HRP
Dot, ELISA, IHC-Fr, IHC-P, IM, WB, ICC/IF
Polyclonal
No
IgG
Liquid
pH: 6.5
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate
Affinity purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Blue Ice
+4°C
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Dot blot analysis of human IgG using ab6858 at 1/1000 dilution.
The indicated qualtities of human IgG were spotted. Blocking was performed for 1 hour at room temperature using a proprietary blocking buffer prior to addition of ab6858.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-MERS-Cov Nucleocapsid protein antibody [abd211] (AB316332) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) conteining a myc-His-tag®, whole cell lysate at 20 µg
Lane 2: 293T transfected with a MERS-CoV Nucleocapsid Protein expression vector conteining a myc-His-tag®, whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Human IgG H&L (HRP) (AB6858) at 1/5000 dilution
Observed band size: 46 kDa, 36 kDa
Exposure time: 1s
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-MERS-Cov Nucleocapsid protein antibody [abd211] (AB316332) at 1/1000 dilution
All lanes: His-tagged MERS-CoV Nucleocapsid recombinant protein at 10 ng
All lanes: Western blot - Goat Anti-Human IgG H&L (HRP) (AB6858) at 1/5000 dilution
Observed band size: 46 kDa
Exposure time: 180s
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-MERS-CoV Nucleocapsid protein antibody [abd208] (AB316330) at 1/1000 dilution
All lanes: His-tagged MERS-CoV Nucleocapsid recombinant protein at 10 ng
All lanes: Western blot - Goat Anti-Human IgG H&L (HRP) (AB6858) at 1/5000 dilution
Developed using the ECL technique.
Observed band size: 46 kDa
Exposure time: 180s
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue sections labelling TAGLN/Transgelin at 1/100 dilution (5 μg/ml) followed by Goat Anti-Human IgG H&L (HRP) (ab6858) secondary antibody at 1/1000 dilution. Negative control: no staining in rat skeletal muscle. The section was incubated with ab213273 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
Immunohistochemical analysis of paraffin-embedded rat lung tissue sections labelling TAGLN/Transgelin at 1/100 dilution (5 μg/ml) followed by Goat Anti-Human IgG H&L (HRP) (ab6858) secondary antibody at 1/1000 dilution. Positive staining on the smooth muscle in rat lung. The section was incubated with ab213273 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
Immunohistochemical analysis of paraffin-embedded rat esophagus tissue sections labelling TAGLN/Transgelin at 1/100 dilution (5 μg/ml) followed by Goat Anti-Human IgG H&L (HRP) (ab6858) secondary antibody at 1/1000 dilution. Positive staining on the smooth muscle in rat esophagus. The section was incubated with ab213273 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) was used at 1:5000 dilution.
All lanes: Western blot - Anti-HCoV-OC43 Nucleoprotein antibody [abd384] (AB309964) at 1/1000 dilution
All lanes: His-tagged HCoV-OC43 Nucleoprotein recombinant protein at 10 ng
All lanes: Western blot - Goat Anti-Human IgG H&L (HRP) (AB6858) at 1/5000 dilution
Observed band size: 65 kDa
Exposure time: 3min
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) was used at 1:200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) was used at 1:5000 dilution.
All lanes: Western blot - Anti-HCoV-OC43 Nucleoprotein antibody [abd384] (AB309964) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cells) transfected with an empty vector containing a His-tag, whole cell lysate at 4 µg
Lane 2: 293T (human embryonic kidney epithelial cells) transfected with HCoV-OC43 Nucleoprotein expression vector containing a His-tag, whole cell lysate at 4 µg
All lanes: Western blot - Goat Anti-Human IgG H&L (HRP) (AB6858) at 1/5000 dilution
Observed band size: 65 kDa
Exposure time: 1s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) was used at 1:5000 dilution.
All lanes: Western blot - Anti-HCoV-OC43 Nucleoprotein antibody [abd385] (AB309966) at 1/1000 dilution
All lanes: His-tagged HCoV-OC43 Nucleoprotein recombinant protein at 10 ng
All lanes: Western blot - Goat Anti-Human IgG H&L (HRP) (AB6858) at 1/5000 dilution
Observed band size: 65 kDa
Exposure time: 3min
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-NL63 Nucleoprotein antibody [abd259] (AB316212) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) conteining a myc-His-tag®, whole cell lysate at 20 µg
Lane 2: 293T cells transfected with an human coronavirus NL63 Nucleoprotein expression vector conteining a myc-His-tag®, whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Human IgG H&L (HRP) (AB6858) at 1/5000 dilution
Observed band size: 50 kDa, 36 kDa
Exposure time: 1s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) was used at 1:200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) was used at 1:5000 dilution.
All lanes: Western blot - Anti-HCoV-OC43 Nucleoprotein antibody [abd385] (AB309966) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cells) transfected with an empty vector containing a His-tag, whole cell lysate at 4 µg
Lane 2: 293T (human embryonic kidney epithelial cells) transfected with HCoV-OC43 Nucleoprotein expression vector containing a His-tag, whole cell lysate at 4 µg
All lanes: Western blot - Goat Anti-Human IgG H&L (HRP) (AB6858) at 1/5000 dilution
Observed band size: 65 kDa
Exposure time: 3.25s
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