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Recombinant secondary antibody suitable for ELISA, WB.

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Images

Western blot - Goat monoclonal RMG01 Anti-Rabbit IgG F(ab) (Biotin) (AB238530), expandable thumbnail
  • ELISA - Goat monoclonal RMG01 Anti-Rabbit IgG F(ab) (Biotin) (AB238530), expandable thumbnail
  • ELISA - Goat monoclonal RMG01 Anti-Rabbit IgG F(ab) (Biotin) (AB238530), expandable thumbnail

Key facts

Host species
Goat
Target species
Rabbit
Target isotype
IgG
Target specificity
F(ab)
Minimal cross-reactivity
Mouse, Rat, Human
Conjugation
Biotin
Applications
ELISA, WB
Clonality
Monoclonal

Reactivity data

Application
ELISA
Reactivity
Reacts
Dilution info
0.01000-0.50000 µg/mL
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
0.10000-0.50000 µg/mL
Notes

Non-reduced

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1 product for Alternative Version

Alternative names

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Recombinant secondary antibody suitable for ELISA, WB.

Key facts

Description
Goat monoclonal RMG01 Anti-Rabbit IgG F(ab) (Biotin)
Host species
Goat
Target species
Rabbit
Target isotype
IgG
Target specificity
F(ab)
Minimal cross-reactivity
Mouse, Rat, Human
Conjugation
Biotin
Applications
ELISA, WB
Clonality
Monoclonal
Clone number
RMG01
Pre-adsorbed
No
Specificity

ab238530 reacts to the Fab region of Rabbit IgG. No cross reactivity with human IgG, rat IgG, or mouse IgG.

Isotype
IgG
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Purification technique
Affinity purification Protein G
Purification notes

ab238530 was purified from an animal origin–free culture supernatant.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Goat monoclonal RMG01 Anti-Rabbit IgG F(ab) (Biotin) (ab238530), expandable thumbnail

    Western blot - Goat monoclonal RMG01 Anti-Rabbit IgG F(ab) (Biotin) (ab238530)

    Lane 1: Non-reduced rabbit IgG at 0.02 µg

    Lane 2: Reduced rabbit IgG at 0.02 µg

    Secondary

    All lanes: Western blot - Goat monoclonal RMG01 Anti-Rabbit IgG F(ab) (Biotin) (ab238530) at 0.2 µg/mL

  • ELISA - Goat monoclonal RMG01 Anti-Rabbit IgG F(ab) (Biotin) (ab238530), expandable thumbnail

    ELISA - Goat monoclonal RMG01 Anti-Rabbit IgG F(ab) (Biotin) (ab238530)

    ELISA of IgGs from different species, shows ab238530 reacts to the Fab region of rabbit IgG; no cross reactivity with human IgG, rat IgG, or mouse IgG. The plate was coated with 50 ng/well of different IgG. ab238530 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.

  • ELISA - Goat monoclonal RMG01 Anti-Rabbit IgG F(ab) (Biotin) (ab238530), expandable thumbnail

    ELISA - Goat monoclonal RMG01 Anti-Rabbit IgG F(ab) (Biotin) (ab238530)

    A titer ELISA of rabbit IgG. The plate was coated with different amounts of rabbit IgG. A serial dilution of ab238530 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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