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AB238530

Goat monoclonal RMG01 Anti-Rabbit IgG F(ab) (Biotin)

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Recombinant secondary antibody suitable for ELISA, WB.
3 Images
ELISA - Goat monoclonal RMG01 Anti-Rabbit IgG F(ab) (Biotin) (AB238530)
  • ELISA

Supplier Data

ELISA - Goat monoclonal RMG01 Anti-Rabbit IgG F(ab) (Biotin) (AB238530)

A titer ELISA of rabbit IgG. The plate was coated with different amounts of rabbit IgG. A serial dilution of ab238530 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.

ELISA - Goat monoclonal RMG01 Anti-Rabbit IgG F(ab) (Biotin) (AB238530)
  • ELISA

Supplier Data

ELISA - Goat monoclonal RMG01 Anti-Rabbit IgG F(ab) (Biotin) (AB238530)

ELISA of IgGs from different species, shows ab238530 reacts to the Fab region of rabbit IgG; no cross reactivity with human IgG, rat IgG, or mouse IgG. The plate was coated with 50 ng/well of different IgG. ab238530 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.

Western blot - Goat monoclonal RMG01 Anti-Rabbit IgG F(ab) (Biotin) (AB238530)
  • WB

Supplier Data

Western blot - Goat monoclonal RMG01 Anti-Rabbit IgG F(ab) (Biotin) (AB238530)

Lane 1:

Non-reduced rabbit IgG at 0.02 µg

Lane 2:

Reduced rabbit IgG at 0.02 µg

Secondary

All lanes:

Western blot - Goat monoclonal RMG01 Anti-Rabbit IgG F(ab) (Biotin) (ab238530) at 0.2 µg/mL

false

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

F(ab)

Minimal cross-reactivity

Mouse, Rat, Human

Pre-adsorbed

No

Conjugation

Biotin

Excitation/Emission
Applications

ELISA, WB

applications

Clonality

Monoclonal

Clone number

RMG01

Isotype

IgG

Specificity

ab238530 reacts to the Fab region of Rabbit IgG. No cross reactivity with human IgG, rat IgG, or mouse IgG.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"0.01-0.5 µg/mL", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"0.1-0.5 µg/mL", "notes":"<p>Non-reduced</p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
ab238530 was purified from an animal origin–free culture supernatant.
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark

Product protocols

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