Recombinant secondary antibody suitable for ELISA, WB.
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info 0.01000-0.50000 µg/mL | Notes - |
Application WB | Reactivity Reacts | Dilution info 0.10000-0.50000 µg/mL | Notes Non-reduced |
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Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS
Recombinant secondary antibody suitable for ELISA, WB.
ab238530 reacts to the Fab region of Rabbit IgG. No cross reactivity with human IgG, rat IgG, or mouse IgG.
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
ab238530 was purified from an animal origin–free culture supernatant.
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Lane 1: Non-reduced rabbit IgG at 0.02 µg
Lane 2: Reduced rabbit IgG at 0.02 µg
All lanes: Western blot - Goat monoclonal RMG01 Anti-Rabbit IgG F(ab) (Biotin) (ab238530) at 0.2 µg/mL
ELISA of IgGs from different species, shows ab238530 reacts to the Fab region of rabbit IgG; no cross reactivity with human IgG, rat IgG, or mouse IgG. The plate was coated with 50 ng/well of different IgG. ab238530 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
A titer ELISA of rabbit IgG. The plate was coated with different amounts of rabbit IgG. A serial dilution of ab238530 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
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