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Recombinant secondary antibody suitable for ELISA.

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Images

ELISA - Goat monoclonal [RMG06] Anti-Mouse IgG Fc (Biotin) (AB272181), expandable thumbnail
  • ELISA - Goat monoclonal [RMG06] Anti-Mouse IgG Fc (Biotin) (AB272181), expandable thumbnail

Key facts

Host species
Goat
Target species
Mouse
Target isotype
IgG
Target specificity
Fc region
Conjugation
Biotin
Applications
ELISA
Clonality
Monoclonal

Reactivity data

Application
ELISA
Reactivity
Reacts
Dilution info
0.05000-1.00000 µg/mL
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Additional Targets

Alternative names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form

Recommended products

Recombinant secondary antibody suitable for ELISA.

Key facts

Description
Goat monoclonal [RMG06] Anti-Mouse IgG Fc (Biotin)
Host species
Goat
Target species
Mouse
Target isotype
IgG
Target specificity
Fc region
Conjugation
Biotin
Applications
ELISA
Clonality
Monoclonal
Clone number
RMG06
Pre-adsorbed
No
Specificity

Goat monoclonal [RMG06] Anti-Mouse IgG Fc (Biotin) (ab272181) reacts to the Fc region of Mouse IgG, including IgG1, IgG2a, and IgG2b. It shows very slight reaction with mouse IgG3. No cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, or rabbit IgG.

Isotype
IgG
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Purification technique
Affinity purification Protein G
Purification notes

Purified from an animal origin–free culture supernatant.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Product promise

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Terms & Conditions.

2 product images

  • ELISA - Goat monoclonal [RMG06] Anti-Mouse IgG Fc (Biotin) (ab272181), expandable thumbnail

    ELISA - Goat monoclonal [RMG06] Anti-Mouse IgG Fc (Biotin) (ab272181)

    ELISA of mouse immunoglobulins shows ab272181 reacts to the Fc region of mouse IgG1, IgG2a, and IgG2b, and very slightly to IgG3; no cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, and rabbit IgG.

    The plate was coated with 50 ng/well of different immunoglobulins. 500 ng/mL, 200 ng/mL, or 50 ng/mL of ab272181 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.

  • ELISA - Goat monoclonal [RMG06] Anti-Mouse IgG Fc (Biotin) (ab272181), expandable thumbnail

    ELISA - Goat monoclonal [RMG06] Anti-Mouse IgG Fc (Biotin) (ab272181)

    A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of ab272181 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.

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Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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