Goat monoclonal [RMG06] Anti-Mouse IgG Fc (Biotin)

• ELISA

Supplier Data

ELISA - Goat monoclonal [RMG06] Anti-Mouse IgG Fc (Biotin) (AB272181)

ELISA of mouse immunoglobulins shows ab272181 reacts to the Fc region of mouse IgG1, IgG2a, and IgG2b, and very slightly to IgG3; no cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, and rabbit IgG.

The plate was coated with 50 ng/well of different immunoglobulins. 500 ng/mL, 200 ng/mL, or 50 ng/mL of ab272181 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.

• ELISA

Supplier Data

ELISA - Goat monoclonal [RMG06] Anti-Mouse IgG Fc (Biotin) (AB272181)

A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of ab272181 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.