AB97233

Goat Anti-Mouse IgA alpha chain (Biotin)

Name: Goat Anti Mouse (IgA alpha chain) secondary antibody Biotin (ab97233) | Abcam
Brand: Abcam Limited
SKU: AB97233

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(6 Publications)

Suitable for WB, ELISA, IHC-P, ICC/IF. Cited in 6 publications.

View Alternative Names

Ig alpha chain C region

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgA

Target specificity

Alpha chain

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Biotin

Excitation/Emission

Applications

IHC-P, WB, ELISA, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgA. Cross reactivity to mouse IgM, IgG1, IgG2a, IgG2b, IgG2c, IgG3 and IgE is undetectable.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/200000", "notes":"(chemiluminescent)." }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/200000", "notes":"(primary)." }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/250 - 1/2500", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/500", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system.

See full target information Ig alpha chain C region

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1654126 PubMed40963609

2025

Upper respiratory tract immunization with PamCys-adjuvanted spike protein vaccine achieves sterilizing protection against SARS-CoV-2.

Applications

Unspecified application

Species

Unspecified reactive species

Erica L Stewart,Skye Stockdale,Matt D Johansen,Lachlan Smith,Sibel Alca,Joshua W C Maxwell,Duc H Nguyen,Stefan Miemczyk,Guanshu Zhao,Stuart Turville,James A Triccas,Megan Steain,Scott N Byrne,Philip M Hansbro,Richard J Payne,Warwick J Britton,Anneliese S Ashhurst

Foods (Basel, Switzerland) 11: PubMed35563952

2022

Stimulates Intestinal Secretion of Immunoglobulin A in an Individual-Specific Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Liya Mei,Ying Chen,Jialiang Wang,Jian Lu,Jianxin Zhao,Hao Zhang,Gang Wang,Wei Chen

Science advances 7: PubMed33658193

2021

Host-microbe cross-talk governs amino acid chirality to regulate survival and differentiation of B cells.

Applications

Unspecified application

Species

Unspecified reactive species

Masataka Suzuki,Tomohisa Sujino,Sayako Chiba,Yoichi Harada,Motohito Goto,Riichi Takahashi,Masashi Mita,Kenji Hamase,Takanori Kanai,Mamoru Ito,Matthew Kaden Waldor,Masato Yasui,Jumpei Sasabe

Immunity 48:120-132.e8 PubMed29343433

2018

Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Johanna Emgård,Hana Kammoun,Bethania García-Cassani,Julie Chesné,Sara M Parigi,Jean-Marie Jacob,Hung-Wei Cheng,Elza Evren,Srustidhar Das,Paulo Czarnewski,Natalie Sleiers,Felipe Melo-Gonzalez,Egle Kvedaraite,Mattias Svensson,Elke Scandella,Matthew R Hepworth,Samuel Huber,Burkhard Ludewig,Lucie Peduto,Eduardo J Villablanca,Henrique Veiga-Fernandes,João P Pereira,Richard A Flavell,Tim Willinger

BioMed research international 2016:4874809 PubMed27298823

2016

Vitamin A Inhibits Development of Dextran Sulfate Sodium-Induced Colitis and Colon Cancer in a Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Isao Okayasu,Kiyomi Hana,Noriko Nemoto,Tsutomu Yoshida,Makoto Saegusa,Aya Yokota-Nakatsuma,Si-Young Song,Makoto Iwata

Oncology letters 11:3579-3582 PubMed27313678

2016

Extraventricular neurocytoma in the left temporal lobe: A case report and review of the literature.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Chen Ji,Jing-Xia Hu,Yan Li,Peng-Xiang Yan,Huan-Cong Zuo

View all publications

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Product promise

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Goat Anti-Mouse IgA alpha chain (Biotin)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Complementary Products

Reagents, Controls & Accessories

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

Product promise