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AB97235

Goat Anti-Mouse IgA alpha chain (HRP)

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(43 Publications)

Goat Anti-Mouse IgA alpha chain (HRP) is a secondary antibody for chemiluminescent detection. Ideal for Western blot. Suitable for Western blot, IHC, ELISA and ICC.

- Reacts specifically with Mouse IgA
- Cross reactivity with other immunoglobulins and light chains is less than 0.1%
- Cross reactivity to mouse IgM, IgG1, IgG2a, IgG2b, IgG2c, IgG3 and IgE is undetectable
- Cited in over 40 publications

View Alternative Names

Ig alpha chain C region

  • Unconjugated

    Goat Anti-Mouse IgA alpha chain

  • 518 DyLight® 488

    Goat Anti-Mouse IgA alpha chain (DyLight® 488)

  • 618 DyLight® 594

    Goat Anti-Mouse IgA alpha chain (DyLight® 594)

  • Biotin

    Goat Anti-Mouse IgA alpha chain (Biotin)

  • 519 FITC

    Goat Anti-Mouse IgA alpha chain (FITC)

  • 576 DyLight® 550

    Goat Anti-Mouse IgA alpha chain (DyLight® 550)

  • 673 DyLight® 650

    Goat Anti-Mouse IgA alpha chain (DyLight® 650)

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgA

Target specificity

Alpha chain

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

HRP

Excitation/Emission
Applications

WB, IHC-P, ELISA, ICC

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgA. Cross reactivity with other immunoglobulins and light chains is less than 0.1%. Cross reactivity to mouse IgM, IgG1, IgG2a, IgG2b, IgG2c, IgG3 and IgE is undetectable. Some hybridoma clones may express aberrant immunoglobulin-related peptides that are improperly recognized by this antibody.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/2000", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/100000", "notes":"<p>(primary).</p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000 - 1/25000", "notes":"<p>Colorimetric: 1/2000 - 1/20000, Chemiluminescent: 1/5000 - 1/25000</p>" } } }

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

Product details

This antibody reacts with the alpha chain of Mouse IgA

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.05% CMIT/MIT based preservative Constituents: 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system.
See full target information Ig alpha chain C region

Publications (43)

Recent publications for all applications. Explore the full list and refine your search

Vaccines 12: PubMed38276664

2024

Antibody Response Following the Intranasal Administration of SARS-CoV-2 Spike Protein-CpG Oligonucleotide Vaccine.

Applications

Unspecified application

Species

Unspecified reactive species

Kentaro Muranishi,Mao Kinoshita,Keita Inoue,Junya Ohara,Toshihito Mihara,Kazuki Sudo,Ken J Ishii,Teiji Sawa,Hiroyasu Ishikura

NPJ vaccines 8:170 PubMed37925490

2023

Both chimpanzee adenovirus-vectored and DNA vaccines induced long-term immunity against Nipah virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Mingqing Lu,Yanfeng Yao,Xuekai Zhang,Hang Liu,Ge Gao,Yun Peng,Miaoyu Chen,Jiaxuan Zhao,XiaoYu Zhang,Chunhong Yin,Weiwei Guo,Peipei Yang,Xue Hu,Juhong Rao,Entao Li,Tong Chen,Sandra Chiu,Gary Wong,Zhiming Yuan,Jiaming Lan,Chao Shan

iScience 26:107747 PubMed37692284

2023

Gut bacteria induce IgA expression in pituitary hormone-secreting cells during aging.

Applications

Unspecified application

Species

Unspecified reactive species

Yehua Li,Jiawen Wang,Rui Wang,Ying Chang,Xiaodong Wang

Nature communications 14:1462 PubMed36927854

2023

B cell class switch recombination is regulated by DYRK1A through MSH6 phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Liat Stoler-Barak,Ethan Harris,Ayelet Peres,Hadas Hezroni,Mirela Kuka,Pietro Di Lucia,Amalie Grenov,Neta Gurwicz,Meital Kupervaser,Bon Ham Yip,Matteo Iannacone,Gur Yaari,John D Crispino,Ziv Shulman

Vaccines 11: PubMed36851165

2023

Identification of Closed Linear Epitopes in S1-RBD and S2-HR1/2 of SARS-CoV-2 Spike Protein Able to Induce Neutralizing Abs.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshihiro Watanabe,Natsuko Hosokawa,Misaki Yoshida,Tomoyuki Miura,Mitsuhiro Kawano

Small (Weinheim an der Bergstrasse, Germany) 19:e2205819 PubMed36564365

2022

Assembly of Immunogenic Protein Particles toward Advanced Synthetic Vaccines.

Applications

Unspecified application

Species

Unspecified reactive species

Shuxiong Chen,Diana H Quan,Gayathri Sam,Victoria Ozberk,Xiaonan T Wang,Peter Halfmann,Manisha Pandey,Michael F Good,Yoshihiro Kawaoka,Warwick J Britton,Bernd H A Rehm

MedComm 3:e188 PubMed36474858

2022

Mixed formulation of mRNA and protein-based COVID-19 vaccines triggered superior neutralizing antibody responses.

Applications

Unspecified application

Species

Unspecified reactive species

Jialu Zhang,Qian He,Xujia Yan,Jianyang Liu,Yu Bai,Chaoqiang An,Bopei Cui,Fan Gao,Qunying Mao,Junzhi Wang,Miao Xu,Zhenglun Liang

Current microbiology 80:2 PubMed36418790

2022

Pal Affects the Proliferation in Macrophages and Virulence of Brucella, and as Mucosal Adjuvants, Provides an Effective Protection to Mice Against Salmonella Enteritidis.

Applications

Unspecified application

Species

Unspecified reactive species

Yubin Chen,Yanfang Fu,Lingcong Kong,Fengjie Wang,Xiaowei Peng,Zhiqiang Zhang,Qiumei Shi,Qingmin Wu,Tonglei Wu

Pharmaceutics 14: PubMed36432730

2022

Formulation of SARS-CoV-2 Spike Protein with CpG Oligodeoxynucleotides and Squalene Nanoparticles Modulates Immunological Aspects Following Intranasal Delivery.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Min Ho,Chiung-Yi Huang,Chung-Hsiang Yang,Shih-Jen Liu,Hsin-Wei Chen,Guann-Yi Yu,Jen-Kun Chen,Tsung-Hsien Chuang,Ming-Hsi Huang

Frontiers in immunology 13:960405 PubMed36341456

2022

Antibody production in mice requires neither vitamin D, nor the vitamin D receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Lori A Plum,William Blaser,Logan Peter,Jean Prahl,Jeremy Seeman,Hector F DeLuca
View all publications

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