Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed
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(2 Publications)
Suitable for IHC-P, ICC/IF, Flow Cyt. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.
View Alternative Names
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed (AB98712)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labeling RILPL1 with ab307294 at 1/500 dilution, followed by ab98712 Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed antibody at 1/1000 dilution (2 µg/ml) (Green). Confocal image showing centrosome and cytoplasmic staining in SH-SY5Y cell line. Low expression : U-937. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (10µg/ml) followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/400 dilution (4 µg/ml) (Red). The nuclear counterstain was DAPI (Blue). -ve control 1 : ab307294 at a 1/500 dilution followed by ab150080 at a 1/500 dilution. -ve control 2 : ab179513 at a 1/200 dilution followed by ab98712 at a 1/1000 dilution.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed (AB98712)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAB8A KO HeLa (RAB8A knockout human cervical adenocarcinoma epithelial cell) (ab264993) cells labeling RAB8A with ab307607 at 1/500 dilution, followed by ab98712 Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed antibody at 1/1000 dilution (2 µg/ml) (Green). Confocal image showing cytoplasmic staining in Parental HeLa cell line, and no staining in RAB8A KO HeLa cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab202272 Recombinant Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab202272) was used to counterstain tubulin at 1/50 dilution (10 µg/ml) (Red). Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab98712 Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed at 1/1000 dilution (2 µg/ml).
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed (AB98712)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labeling RAB8A with ab307607 at 1/500 dilution, followed by ab98712 Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed antibody at 1/1000 dilution (2 µg/ml) (Green). Confocal image showing cytoplasmic staining in A549 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab202272 Recombinant Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab202272) was used to counterstain tubulin at 1/50 dilution (10 ug/ml) (Red). Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab98712 Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed at 1/1000 dilution (2 µg/ml).
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed (AB98712)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labeling RILPL1 with ab307294 at 1/500 dilution, followed by ab98712 Goat Anti-Mouse IgG Fc (DyLight® 488) preadsorbed antibody at 1/1000 dilution (2 µg/ml) (Green). Confocal image showing centrosome staining in Neuro-2a cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (10µg/ml) followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/400 dilution (4 µg/ml) (Red). The nuclear counterstain was DAPI (Blue). -ve control 1 : ab307294 at a 1/500 dilution followed by ab150080 at a 1/500 dilution. -ve control 2 : ab179513 at a 1/200 dilution followed by ab98712 at a 1/1000 dilution.
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Publications (2)
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Pest management science 80:5364-5376 PubMed39031631
2024
Applications
Unspecified application
Species
Unspecified reactive species
Genome biology 22:189 PubMed34167554
2021
Applications
Unspecified application
Species
Unspecified reactive species
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