Goat Anti-Mouse IgG Fc (HRP)

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG Fc (HRP) (AB97265)

IHC image of beta actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8224, 3μg/ml overnight at +4°C. An HRP-conjugated secondary (ab97265, 1/1000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

The inset negative control image is secondary-only at 1/200 dilution.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• WB

AbReview58470****

Western blot - Goat Anti-Mouse IgG Fc (HRP) (AB97265)

All lanes:

Western blot - Anti-SMN/Gemin 1 antibody [2B1] (<a href='/en-us/products/primary-antibodies/smn-gemin-1-antibody-2b1-ab5831'>ab5831</a>) at 1/100 dilution

All lanes:

Xenopus laevis st II-III oocytes whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG Fc (HRP) (ab97265) at 1/25000 dilution

Predicted band size: 32 kDa

Observed band size: 31 kDa

false

Exposure time: 30s

This image is courtesy of an abreview submitted by Samantha Jeschonek.