Suitable for ICC, IHC-P, WB, ELISA. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.
Images
Publications
Filter by
- Journal of Parkinson's disease 11:1791-18032021DNAJB6b is Downregulated in Synucleinopathies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJonas Folke,Sertan Arkan,Isak Martinsson,Susana Aznar,Gunnar Gouras,Tomasz Brudek,Christian HansenPubMed 34334418
- Scientific reports 10:97502020A cross-reactive mouse monoclonal antibody against rhinovirus mediates phagocytosis in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohammad Amin Behzadi et. al.PubMed 32546721
Key facts
- Host species
- Goat
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Fc region
- Minimal cross-reactivity
- Rat, Human
- Conjugation
- HRP
- Applications
- ICC, IHC-P, WB, ELISA
- Clonality
- Polyclonal
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/200.00000 - 1/2000.00000 | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/2000.00000 - 1/25000.00000 | Notes (Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000). |
Application ELISA | Reactivity Reacts | Dilution info 1/10000.00000 - 1/100000.00000 | Notes (Primary). |
Target data
Additional Targets
Alternative names
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form
Recommended products
Suitable for ICC, IHC-P, WB, ELISA. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.
Key facts
- Description
- Goat Anti-Mouse IgG Fc (HRP) preadsorbed
- Host species
- Goat
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Fc region
- Minimal cross-reactivity
- Rat, Human
- Conjugation
- HRP
- Applications
- ICC, IHC-P, WB, ELISA
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgG was detected. This antibody may cross react with IgG from other species.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.05% CMIT/MIT based preservative
Constituents: 0.2% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
20 product images
Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717)
This data was developed using Anti-Myc tag antibody [Hyper-myc] - Mouse IgG2a (Chimeric) ab289980, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST
Anti-Myc tag antibody [Hyper-myc] - Mouse IgG2a (Chimeric) ab289980 detects a ranged of myc tagged proteins in WB.
All lanes: Western blot - Anti-Myc tag antibody [Hyper-myc] - Mouse IgG2a (Chimeric) (Anti-Myc tag antibody [Hyper-myc] - Mouse IgG2a (Chimeric) ab289980) at 1/5000 dilution
Lane 1: HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 1 µg
Lane 2: HEK-293T transfected with cas9 ( S. Aureas) expression vector containing a myc-His-tag®, whole cell lysate at 1 µg
Lane 3: HEK-293T transfected with human SNCA140 expression vector containing a myc-His-tag®, whole cell lysate at 1 µg
Lane 4: HEK-293T transfected with human IDS expression vector containing a myc-His-tag®, whole cell lysate at 1 µg
Lane 5: HEK-293T transfected with human NF2 S518A expression vector containing a myc-His-tag®, whole cell lysate at 1 µg
Lane 6: HEK-293T transfected with human Alphasynuclein expression vector containing a myc-His-tag®, whole cell lysate at 1 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717) at 1/10000 dilution
Predicted band size: 49 kDa
Exposure time: 3s
Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717)
All lanes: Western blot - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) ab289864) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg
Lane 3: HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 20 µg
Lane 4: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 5: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg
Lane 6: A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate at 20 µg
Lane 7: C2C12 (mouse myoblasts myoblast), whole cell lysate at 20 µg
Lane 8: C6 (rat glial tumor glial cell), whole cell lysate at 20 µg
Lane 9: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysa at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717) at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 52 kDa
Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-RILPL1 antibody [MJF-R41-21] - Mouse IgG2a (Chimeric) ab307294 was shown to bind specifically to RILPL1. A band was observed at 47 kDa in wild-type A549 cell lysates with whereas no signal observed at this size in RILPL1 knockout cell line.
Wild-type and RILPL1 knockout A549 cell lysates were kindly provided by Dr. Dario Alessi, University of Dundee.
Exposure time: 3 minutes.All lanes: Western blot - Anti-RILPL1 antibody [MJF-R41-21] - Mouse IgG2a (Chimeric) (Anti-RILPL1 antibody [MJF-R41-21] - Mouse IgG2a (Chimeric) ab307294) at 1/1000 dilution
Lane 1: Wild-type A549 (human lung carcinoma epithelial cell), whole cell lysate 20 μg at 20 µg
Lane 2: RILPL1 knockout A549 (human lung carcinoma epithelial cell), whole cell lysate 20 μg at 20 µg
Lane 3: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 20 μg at 20 µg
Lane 4: MEF (mouse embryonic fibroblast (immortalized)), whole cell lysate 20 μg at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717) at 1/20000 dilution
Observed band size: 47 kDa
Exposure time: 3min
Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717)
Negative control: Jurkat (PMID: 25108024).
The expression profile observed in Jurkat is consistent with the literature (PMID: 25108024).
Exposure time: 26 seconds.All lanes: Western blot - Anti-LAG-3 antibody [EPR20261] - Mouse IgG1 (Chimeric) (Anti-LAG-3 antibody [EPR20261] - Mouse IgG1 (Chimeric) ab307517) at 1/1000 dilution
Lane 1: HDLM-2 (human Hodgkin lymphoma) whole cell lysate 20 μg
Lane 2: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate 20 μg
Observed band size: 54 kDa, 70 kDa
Exposure time: 26s
Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-RAB8A antibody [MJF-R22-79-3] - Mouse IgG2a (Chimeric) ab307607 was shown to bind specifically to RAB8A. A band was observed at 23 kDa in wild-type HeLa cell lysates with whereas no signal observed at this size in RAB8A knockout cell line Human RAB8A knockout HeLa cell line ab264993 (knockout cell lysate Human RAB8A knockout HeLa cell lysate ab257195).
Exposure time: 48 seconds.All lanes: Western blot - Anti-RAB8A antibody [MJF-R22-79-3] - Mouse IgG2a (Chimeric) (Anti-RAB8A antibody [MJF-R22-79-3] - Mouse IgG2a (Chimeric) ab307607) at 1/5000 dilution
Lane 1: Wild-type HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 20 μg
Lane 2: RAB8A knockout HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 20 μg
Lane 3: HCT116 (human colorectal carcinoma epithelial cell)whole cell lysate 20 μg
Lane 4: A549 (human lung carcinoma epithelial cell), whole cell lysate 20 μg
Observed band size: 23 kDa
Exposure time: 48s
Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: Lanes 1-3: 48 seconds；Lanes 4-9: 92 seconds；Lanes 10-13: 37 secondsAll lanes: Western blot - Anti-APG5L/ATG5 antibody [EPR1755(2)] - Mouse IgG1 (Chimeric) (Anti-APG5L/ATG5 antibody [EPR1755(2)] - Mouse IgG1 (Chimeric) ab307842) at 1/1000 dilution
Lane 1: HT-1080 (human fibrosarcoma epithelial cell)whole cell lysate at 20 µg
Lane 2: HeLa (human cervix adenocarcinoma epithelial cell)whole cell lysate at 20 µg
Lane 3: Mouse brain tissue lysate at 10 µg
Lane 4: Mouse heart tissue lysate at 10 µg
Lane 5: Mouse kidney tissue lysate at 10 µg
Lane 6: Mouse spleen tissue lysate at 10 µg
Lane 7: Rat brain tissue lysate at 10 µg
Lane 8: Rat heart tissue lysate at 10 µg
Lane 9: C6 (rat glial tumor glial cell)whole cell lysate at 10 µg
Lane 10: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage)whole cell lysate at 10 µg
Lane 11: PC-12 (rat adrenal gland pheochromocytoma)whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717) at 1/20000 dilution
Observed band size: 55 kDa
Exposure time: 48s
Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: Kidney and heart. (PMID: 14997013).
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 37 seconds.All lanes: Western blot - Anti-Alpha-synuclein antibody [MJFR1] - Mouse IgG2a (Chimeric) (Anti-Alpha-synuclein antibody [MJFR1] - Mouse IgG2a (Chimeric) ab309157) at 1/1000 dilution
Lane 1: Human cerebellum tissue lysate at 10 µg
Lane 2: Human heart tissue lysate at 10 µg
Lane 3: Human kidney tissue lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717) at 1/20000 dilution
Observed band size: 18 kDa
Exposure time: 37s
Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression tissues: Kidney and heart (PMID:11604140).
In Western blot, anti- Vinculin antibody (Anti-Vinculin antibody [EPR8185] ab129002) loading control staining at 1/10000 dilution.
Exposure time: 1 second.All lanes: Western blot - Anti-Synaptophysin antibody [YE269] - Mouse IgG1 (Chimeric) (Anti-Synaptophysin antibody [YE269] - Mouse IgG1 (Chimeric) ab309493) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse cerebellum tissue lysate at 20 µg
Lane 3: Mouse hippocampus tissue lysate at 20 µg
Lane 4: Mouse heart tissue lysate at 20 µg
Lane 5: Mouse kidney tissue lysate at 20 µg
Lane 6: Rat brain tissue lysate at 20 µg
Lane 7: Rat cerebellum tissue lysate at 20 µg
Lane 8: Rat heart tissue lysate at 20 µg
Lane 9: Rat kidney tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717) at 1/20000 dilution
Observed band size: 38 kDa
Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression tissue: Kidney (PMID:11604140).
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 8 seconds.All lanes: Western blot - Anti-Synaptophysin antibody [YE269] - Mouse IgG1 (Chimeric) (Anti-Synaptophysin antibody [YE269] - Mouse IgG1 (Chimeric) ab309493) at 1/1000 dilution
Lane 1: Human cerebellum tissue lysate at 10 µg
Lane 2: Human kidney tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717) at 1/20000 dilution
Observed band size: 38 kDa
Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Performed under reducing conditions.
In Western blot, Anti-Synaptophysin antibody [YE269] - Mouse IgG1 (Chimeric) ab309493 was shown to bind specifically to Synaptophysin (SYP). Target of interest was observed at 38 kDa wild-type 293T cell lysates (lane 1) with no signal observed at this size in SYP knockout cell line (lane 2, knockout cell line Human SYP (Synaptophysin) knockout HEK-293T cell line ab267272 / knockout cell lysate Human SYP (Synaptophysin) knockout HEK-293T cell lysate ab257060).
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 59 seconds.All lanes: Western blot - Anti-Synaptophysin antibody [YE269] - Mouse IgG1 (Chimeric) (Anti-Synaptophysin antibody [YE269] - Mouse IgG1 (Chimeric) ab309493) at 1/1000 dilution
Lane 1: Wild-type 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 2: Western blot - Human SYP (Synaptophysin) knockout HEK-293T cell lysate (Human SYP (Synaptophysin) knockout HEK-293T cell lysate ab257060) at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717) at 1/20000 dilution
Observed band size: 38 kDa
Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717)
Blocking and diluting buffer and concentration: 5% NFDM/TBST Exposure time: 26 seconds
All lanes: Western blot - Anti-Cathepsin D [EPR3057Y] - Mouse IgG2a (Chimeric) (Anti-Cathepsin D [EPR3057Y] - Mouse IgG2a (Chimeric) ab302649) at 1/1000 dilution
Lane 1: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 20 µg
Lane 3: SK-BR-3 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 4: U937 (human histiocytic lymphoma monocyte), whole cell lysate at 20 µg
Lane 5: A431 (human epidermoid carcinoma epithelial cell), whole cell lysate at 20 µg
Lane 6: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg
Lane 7: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg
Lane 8: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717) at 1/20000 dilution
Observed band size: 28 kDa, 14 kDa, 46 kDa
Exposure time: 26s
Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717)
Blocking and diluting buffer and concentration: 5% NFDM/TBST Exposure time: Lane 1-3: 15 seconds
Lane 4, 5: 125 secondsAll lanes: Western blot - Anti-Cathepsin D [EPR3057Y] - Mouse IgG2a (Chimeric) (Anti-Cathepsin D [EPR3057Y] - Mouse IgG2a (Chimeric) ab302649) at 1/1000 dilution
Lane 1: Human heart tissue lysate at 20 µg
Lane 2: Human liver tissue lysate at 20 µg
Lane 3: Human spleen tissue lysate at 20 µg
Lane 4: Mouse brain tissue lysate at 20 µg
Lane 5: Rat stomach tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717) at 1/20000 dilution
Observed band size: 14 kDa, 28 kDa, 46 kDa
Exposure time: 15s
Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: U937 (Human Protein Atlas).
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes: Western blot - Anti-RILPL1 antibody [MJF-R41-21] - Mouse IgG2a (Chimeric) (Anti-RILPL1 antibody [MJF-R41-21] - Mouse IgG2a (Chimeric) ab307294) at 1/1000 dilution
Lane 1: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 20 µg
Lane 2: HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 20 µg
Lane 3: U937 (human histiocytic lymphoma monocyte), whole cell lysate at 20 µg
Lane 4: Human cerebellum tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717) at 1/20000 dilution
Developed using the ECL technique.
Observed band size: 47 kDa
Exposure time: 81s
Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
Exposure time:
Lane 1-4: 180 seconds
Lanes 5-7: 125 seconds
All lanes: Western blot - Anti-RILPL1 antibody [MJF-R41-21] - Mouse IgG2a (Chimeric) (Anti-RILPL1 antibody [MJF-R41-21] - Mouse IgG2a (Chimeric) ab307294) at 1/1000 dilution
Lane 1: C6 (rat glial tumor cell), whole cell lysate at 10 µg
Lane 2: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg
Lane 3: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg
Lane 4: Rat brain tissue lysate at 10 µg
Lane 5: Mouse brain tissue lysate at 10 µg
Lane 6: Mouse heart tissue lysate at 10 µg
Lane 7: Mouse spleen tissue lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717) at 1/20000 dilution
Developed using the ECL technique.
Observed band size: 47 kDa
Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] - Mouse IgG2a (Chimeric) (Anti-RAB10 (phospho T73) antibody [MJF-R21] - Mouse IgG2a (Chimeric) ab315174) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc-His-tag, whole cell lysate at 20 µg
Lane 2: 293T transfected with human RAB10 expression vector containing a myc-His-tag, whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 23 kDa, 36 kDa
Exposure time: 180s
Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] - Mouse IgG2a (Chimeric) (Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] - Mouse IgG2a (Chimeric) ab315172) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc-His-tag, whole cell lysate at 20 µg
Lane 2: 293T transfected with human RAB10 expression vector containing a myc-His-tag, whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717) at 1/100000 dilution
Observed band size: 23 kDa, 36 kDa
Exposure time: 92s
Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 15 secondsAll lanes: Western blot - Anti-RAB10 antibody [MJF-R23] - Mouse IgG2a (Chimeric) (Anti-RAB10 antibody [MJF-R23] - Mouse IgG2a (Chimeric) ab307296) at 1/1000 dilution
Lane 1: A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 3: HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 5: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717) at 1/20000 dilution
Observed band size: 22 kDa
Exposure time: 15s
Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, Anti-RAB10 antibody [MJF-R23] - Mouse IgG2a (Chimeric) ab307296 was shown to bind specifically to RAB10. A band was observed at 22 kDa in wild-type A549 cell lysates with whereas no signal observed at this size in RAB10 knockout cell line.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 10 secondsAll lanes: Western blot - Anti-RAB10 antibody [MJF-R23] - Mouse IgG2a (Chimeric) (Anti-RAB10 antibody [MJF-R23] - Mouse IgG2a (Chimeric) ab307296) at 1/1000 dilution
Lane 1: Wild-type A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: RAB10 knockout A549 whole cell lysate at 20 µg
Lane 3: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717) at 1/20000 dilution
Observed band size: 22 kDa
Exposure time: 10s
Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717)
All lanes: Western blot - Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) (Anti-Beclin 1 antibody [EPR19662] - Mouse IgG2a (Chimeric) ab289864) at 1/1000 dilution
Lane 1: Human liver tissue lysate at 20 µg
Lane 2: Mouse heart tissue lysate at 20 µg
Lane 3: Mouse spleen tissue lysate at 20 µg
Lane 4: Rat heart tissue lysate at 20 µg
Lane 5: Rat spleen tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717) at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 52 kDa
Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717)
All lanes: Western blot - Anti-C5a / C5a des Arg antibody [C17/5] (Anti-C5a / C5a des Arg antibody [C17/5] ab303662) at 1/1000 dilution
Lane 1: Human plasma lysate at 20 µg
Lane 2: Human skeletal muscle tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (ab98717) at 1/5000 dilution
Observed band size: 110 kDa
Exposure time: 60s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com