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Goat Anti-Mouse IgG H&L is a unconjugated secondary antibody that is suitable for Conjugation, Western blot, IHC, ICC/IF, IP, ELISA and more.

- Cited in over 60 publications

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Images

Western blot - Goat Anti-Mouse IgG H&L (AB6708), expandable thumbnail
  • Western blot - Goat Anti-Mouse IgG H&L (AB6708), expandable thumbnail

Publications

Key facts

Host species
Goat
Target species
Mouse
Target isotype
IgG
Target specificity
Heavy & Light chains
Applications
Conjugation, IHC-Fr, IHC-P, IP, ID, ICC/IF, WB, ELISA
Clonality
Polyclonal

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Reactivity data

Application
Conjugation
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC-Fr
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC-P
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IP
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ID
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ICC/IF
Reactivity
Reacts
Dilution info
-
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
1/3000 - 1/15000
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
1/25000
Notes

-

Additional Targets

Alternative names

Recommended products

Goat Anti-Mouse IgG H&L is a unconjugated secondary antibody that is suitable for Conjugation, Western blot, IHC, ICC/IF, IP, ELISA and more.

- Cited in over 60 publications

Key facts

Description
Goat Anti-Mouse IgG H&L
Host species
Goat
Target species
Mouse
Target isotype
IgG
Target specificity
Heavy & Light chains
Applications
Conjugation, IHC-Fr, IHC-P, IP, ID, ICC/IF, WB, ELISA
Clonality
Polyclonal
Pre-adsorbed
No
Isotype
IgG
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Purification technique
Affinity purification
Purification notes

Secondary Antibody Anti-Mouse IgG (H&L) was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This antibody reacts with the heavy and light chains of Mouse IgG.

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies , designed to deliver precise and reliable results.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Goat Anti-Mouse IgG H&L (ab6708), expandable thumbnail

    Western blot - Goat Anti-Mouse IgG H&L (ab6708)

    ab6708 incubation overnight at 4°C.

    Both heavy (55kDa) and light (28kDa) chains are detected.

    All lanes: Mouse IgG at 0.1 µg

    Secondary

    Lane 1: Secondary antibody: DyLight™ 800 goat secondary antibody at 1/20000 dilution

    Lane 1: Western blot - Goat Anti-Mouse IgG H&L (ab6708) at 1/1000 dilution

  • Western blot - Goat Anti-Mouse IgG H&L (ab6708), expandable thumbnail

    Western blot - Goat Anti-Mouse IgG H&L (ab6708)

    ab6708 incubated for 1 hour at room temperature.

    Lane 1: Mouse IgG (whole molecule) at 0.05 µg

    Lane 2: Mouse IgG Fc fragment at 0.05 µg

    Lane 3: Mouse IgG Fab fragment at 0.05 µg

    Lane 4: Mouse IgM kappa at 0.05 µg

    Lane 5: Mouse serum at 0.05 µg

    Secondary

    Lanes 1 - 5: HRP Donkey Anti-Goat IgG at 1/40000 dilution

    Lanes 1 - 5: Western blot - Goat Anti-Mouse IgG H&L (ab6708) at 1/1000 dilution

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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