Goat Anti-Mouse IgG H&L is a unconjugated secondary antibody that is suitable for Conjugation, Western blot, IHC, ICC/IF, IP, ELISA and more.
- Cited in over 60 publications
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application Conjugation | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application IP | Reactivity Reacts | Dilution info - | Notes - |
Application ID | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/3000 - 1/15000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/25000 | Notes - |
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form
Goat Anti-Mouse IgG H&L is a unconjugated secondary antibody that is suitable for Conjugation, Western blot, IHC, ICC/IF, IP, ELISA and more.
- Cited in over 60 publications
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Secondary Antibody Anti-Mouse IgG (H&L) was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose.
This antibody reacts with the heavy and light chains of Mouse IgG.
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Full details and terms and conditions can be found here:
Terms & Conditions.
ab6708 incubation overnight at 4°C.
Both heavy (55kDa) and light (28kDa) chains are detected.
All lanes: Mouse IgG at 0.1 µg
Lane 1: Secondary antibody: DyLight™ 800 goat secondary antibody at 1/20000 dilution
Lane 1: Western blot - Goat Anti-Mouse IgG H&L (ab6708) at 1/1000 dilution
ab6708 incubated for 1 hour at room temperature.
Lane 1: Mouse IgG (whole molecule) at 0.05 µg
Lane 2: Mouse IgG Fc fragment at 0.05 µg
Lane 3: Mouse IgG Fab fragment at 0.05 µg
Lane 4: Mouse IgM kappa at 0.05 µg
Lane 5: Mouse serum at 0.05 µg
Lanes 1 - 5: HRP Donkey Anti-Goat IgG at 1/40000 dilution
Lanes 1 - 5: Western blot - Goat Anti-Mouse IgG H&L (ab6708) at 1/1000 dilution
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