Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

41 product images

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neurons labelling Synaptophysin with Anti-Synaptophysin antibody [YE269] - Mouse IgG1 (Chimeric) ab309493 at 1/100 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).
  
  Confocal image showing cytoplasmic staining in mouse primary neuron cell line.
  
  Confocal scanning Z step was set as 0.3 µM followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Anti-MAP2 antibody [EPR19691] - Neuronal Marker ab183830 Anti-MAP2 rabbit monoclonal antibody was used to counterstain tubulin at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).
  
  Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

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  Flow Cytometry (Intracellular) - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Overlay histogram showing SV40LT-SMC cells stained with Anti-alpha smooth muscle Actin antibody [1A4] ab7817 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (Anti-alpha smooth muscle Actin antibody [1A4] ab7817 1.137μg/ml) for 30 min at 22°C. The secondary antibody used was Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) (ab150113) at 1/2000 dilution for 30 min at 22°C

  Isotype control antibody (black line) was mouse IgG2a [18C8BC7AD10] (Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control ab170191) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

  Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

  This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

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  Flow Cytometry (Intracellular) - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Overlay histogram showing HeLa cells stained with Anti-PCNA antibody [PC10] ab29 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween 20 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-PCNA antibody [PC10] ab29, 0.1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control ab91361, 0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.
  Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

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  Flow Cytometry - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Overlay histogram showing HeLa cells stained with Anti-p53 antibody [PAb 240] ab26 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-p53 antibody [PAb 240] ab26, 1μg/1x10^6 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-mouse IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  ICC/IF image of Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, 1μg/ml) overnight at +4°C. The secondary antibody (green) was ab150113 Alexa Fluor^® 488 goat anti-mouse IgG (H+L) used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
  

  The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 cells labelling Mouse IgG1 with Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control ab280974 at 1/20 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Anti-beta Tubulin antibody [EPR16774] - Loading Control ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
  

  Confocal image showing no staining in RAW 264.7 cells.

  Negative control 1: Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control ab280974 at a 1/20 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 at a 1/1000 dilution.

  Negative control 2: Anti-beta Tubulin antibody [EPR16774] - Loading Control ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.

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  Alexa Fluor® - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
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  Flow Cytometry (Intracellular) - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling CTSD (cathepsin D) with Anti-Cathepsin D [EPR3057Y] - Mouse IgG2a (Chimeric) ab302649 at 1/1000 dilution (0.1ug) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-Mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labeling Bassoon/BSN with Anti-Bassoon/BSN antibody [L124-59] ab306593 at 1/50 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/ml) (Green).
  Confocal image showing cytoplasmic staining in mouse primary neuron.
  Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. Anti-MAP2 antibody [EPR19691] - Neuronal Marker ab183830 Anti-MAP2 rabbit monoclonal antibody was used to counterstain tubulin at 1/1000 dilution (1 µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088) at a 1/500 dilution (2 µg/ml) (Red). The nuclear counterstain was DAPI (Blue).
  
  -ve control 1: Anti-Bassoon/BSN antibody [L124-59] ab306593 at a 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088 at a 1/500 dilution.
  
  -ve control 2: Anti-MAP2 antibody [EPR19691] - Neuronal Marker ab183830 at a 1/1000 dilution followed by ab150113 at a 1/1000 dilution.

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  Flow Cytometry (Intracellular) - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Flow cytometric (Intracellular) overlay histogram showing 4% paraformaldehyde fixed, 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labeling Ubiquitin with Anti-Ubiquitin antibody [P4D1] ab303664 at 1/800 dilution (0.1μg) (red line). Isotype control antibody (black line) was a mouse monoclonal IgG used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.

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  Flow Cytometry (Intracellular) - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Flow cytometric (Intracellular) overlay histogram showing 4% paraformaldehyde fixed, 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labeling Ubiquitin with Anti-Ubiquitin antibody [P4D1] ab303664 at 1/800 dilution (0.1μg) (red line). Isotype control antibody (black line) was a mouse monoclonal IgG used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.

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  Flow Cytometry (Intracellular) - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling NFkB p105 / p50 with Anti-NFkB p105 / p50 antibody [1298CT792.105.117.133] ab305263 at 1/100 dilution (1µg) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).
  
  Goat anti-Mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

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  Flow Cytometry (Intracellular) - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Raji (human Burkitt's lymphoma B lymphocyte) cells labeling NFkB p105 / p50 with Anti-NFkB p105 / p50 antibody [1298CT792.105.117.133] ab305263 at 1/100 dilution (1µg) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).
  
  Goat anti-Mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  MCF7/ HCT 116 cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] ab70475 at 1:100 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti-Mouse secondary antibody (ab150113) at 1/1000 dilution at RT for 45 min. Anti-beta Tubulin antibody [EPR16774] - Loading Control ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with Anti-Integrin beta 3 antibody [Y2/51] ab9509 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
  
  Confocal image showing membranous and cytoplasmic staining in MCF7 cells.
  Negative control: HCT 116 (PMID: 14998492)

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labeling MAP2 with Alexa Fluor® 568 Anti-MAP2 antibody [EPR19691] - Neuronal Marker ab303465 at 1/250 dilution (2 ug/ml) (Green). Confocal image showing positive staining in rat primary neuron. Confocal scanning Z step was set as 0.3 μm, followed by image processing with maximum Z projection. Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 μg/ml dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (Green). The Nuclear counterstain was DAPI (Blue).

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labeling MAP2 with Alexa Fluor® 568 Anti-MAP2 antibody [EPR19691] - Neuronal Marker ab303465 at 1/250 dilution (2 μg/ml) (Red). Confocal image showing positive staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 μg/ml dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (Green). The Nuclear counterstain was DAPI (Blue).

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neurons labelling Synaptophysin with Anti-Synaptophysin antibody [YE269] - Mouse IgG1 (Chimeric) ab309493 at 1/100 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).
  
  Confocal image showing cytoplasmic staining in rat primary neuron cell line.
  
  Confocal scanning Z step was set as 0.3 µM followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Anti-MAP2 antibody [EPR19691] - Neuronal Marker ab183830 Anti-MAP2 rabbit monoclonal antibody was used to counterstain tubulin at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).
  
  Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human cervical adenocarcinoma epithelial cells labeling MTCO1 with Anti-MTCO1 antibody [1D6E1A8] ab14705 at 1/100 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution. Confocal image showing cytoplasmic and membranous staining in subsets of Human cervical adenocarcinoma epithelial cells. Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab186735 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker was used to counterstain tubulin at 1/400 dilution. The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse neuroblastoma neuroblast cells labeling MTCO1 with Anti-MTCO1 antibody [1D6E1A8] ab14705 at 1/100 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution. Confocal image showing cytoplasmic and membranous staining in subsets of Mouse neuroblastoma neuroblast cells. Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab186735 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker was used to counterstain tubulin at 1/400 dilution. The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Rat glial tumor cells labeling MTCO1 with Anti-MTCO1 antibody [1D6E1A8] ab14705 at 1/100 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution. Confocal image showing cytoplasmic and membranous staining in subsets of Rat glial tumor cells. Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab186735 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker was used to counterstain tubulin at 1/400 dilution. The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized LNCaP (human prostate carcinoma epithelial cell) cells labelling TMPRSS2 with Anti-TMPRSS2 antibody [P5H9-A3] ab309546 at 1/100 (10.28 ug/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in LNCaP and negative staining in 293T (PMID: 33493182).Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
  antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

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  Immunohistochemistry (Frozen sections) - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse spleen (fresh) tissue labeling CD32B with Anti-CD32B antibody [AT130-5] ab290737 at 1/100 (11.1 μg/ml) dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2 μg/ml) (Green). Positive staining on mouse spleen is observed. The nuclear counterstain was DAPI (Blue).
  
  Secondary antibody control:  PBS was used instead of primary antibody followed by secondary antibody ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)at 1/1000 dilution (2 μg/ml).

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  Immunohistochemistry (Frozen sections) - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse pituitary (fresh frozen) tissue labeling ACTH with Anti-ACTH antibody [AH26] ab233953 at 1/100 (11.64 µg/ml) dilution followed by ab150113, Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green).

  Positive staining on mouse pituitary. The nuclear counterstain was DAPI (Blue).

  Secondary antibody control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluorr^® 488) at 1/1000 dilution.

  Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

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  Immunohistochemistry (Frozen sections) - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat spleen (fresh) tissue labeling CD2 with Anti-CD2 antibody [OX34] ab243837 at 1/100 (10.43 ug/ml) dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 (2 ug/mL) dilution (Green).

  Confocal image showing positive staining on rat spleen. The nuclear counterstain was DAPI (Blue).

  Secondary antibody control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 (2 ug/mL) dilution

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized Calu-3 (human lung adenocarcinoma epithelial cell) labelling (A) MUC5B with Anti-MUC5B antibody [EPR27200-159] ab315330 at 1/500 (1.008 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green), or (B) MUC5AC with Anti-Mucin 5AC antibody [45M1] ab3649 at 1/100 (10.45 ug/ml) followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

  This antibody does not cross-react with human MUC5AC.
  
  (A) No staining of MUC5B in Calu-3 cell line.
  
  (B) Positive staining of MUC5AC (Anti-Mucin 5AC antibody [45M1] ab3649, green) in Calu-3 cell line.
  

  Images were taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

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  Immunohistochemistry (Frozen sections) - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized fresh frozen rat cerebrum tissue labeling GFAP with Anti-GFAP antibody [EPR1034Y] - Mouse IgG2a (Chimeric) ab279290 at 1/200 (10 µg/ml) dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 (2μg/mL) dilution. Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (blue).

  Secondary antibody control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

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  Immunohistochemistry (Frozen sections) - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized fresh frozen mouse cerebrum tissue labeling GFAP with Anti-GFAP antibody [EPR1034Y] - Mouse IgG2a (Chimeric) ab279290 at 1/200 (10 µg/ml) dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 (2μg/mL) dilution. Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (blue).

  Secondary antibody control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling influenza A H1N1 (A/California/07/2009) neuraminidase with Anti-influenza A H1N1 (A/California/07/2009) neuraminidase antibody [CD6] ab318988 at 1/2000 (0.465 ug/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (2ug/ml) dilution (Green).

  Confocal image showing cytoplasmic and membranous staining in 293T cells transfected with a N1, H1N1, A/California/07/2009 expression vector containing a myc-His-tag® (shown in green) but no staining in 293T cells transfected with a N9, H7N9, A/Anhui/1/2013 or N1, H1N1, A/Brisbane/59/2007 vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
  
  A: Myc transfected cells
  
  B: N1, H1N1, A/Brisbane/59/2007-Myc transfected cells
  
  C: N1, H1N1, A/California/07/2009-Myc transfected cells
  
  D: N9, H7N9, A/Anhui/1/2013-Myc transfected cells
  
  Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Magenta). The nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 (2ug/ml) dilution.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus type 59 L1 protein with Anti-HPV 59 L1 antibody [FR_αHPV59L1_6E12] ab322271 at 1/2000 (0.49ug/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2ug/ml dilution (Green).

  Confocal image showing positive staining in 293T cells transfected with a Human papillomavirus type 59 (HPV59) L1 protein expression vector containing a myc-His-tag®(shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 2.5 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 2ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling Hsp27 with Anti-Hsp27 antibody [G3.1] ab2790 at 1/100 dilution, followed by AlexaFluor® 488-conjugated Goat anti-Mouse secondary antibody (ab150113) at 1/1000 dilution at RT for 45 min. Anti-beta Tubulin antibody [EPR16774] - Loading Control ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with Anti-Integrin beta 3 antibody [Y2/51] ab9509 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized BxPC-3 (human pancreas adenocarcinoma epithelial cell) cells labelling Lewis a,b carbohydrate with Anti-Lewis a,b carbohydrate antibody [HEA164] ab322960 at 1/2000 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

  Confocal image showing cytoplasmic and membranous staining in BxPC-3 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
  
  Negative control: HepG2.
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] - Microtubule Marker ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

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  Flow Cytometry - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Flow Cytometry analysis of human monocyte-derived dendritic cells treated with 100ng/mL Lipopolysaccharide (LPS) for 24 hours (Right) / Untreated control (Left).
  
  Anti-CD83 antibody [HB15e] ab123494 used at a 1/1000 dilution.
  
  Secondary is Goat anti-Mouse IgG (Alexa Fluor® 488, ab150113) used at a 1/2000 dilution.
  
  The cells were simultaneously stained with CD209. Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
  Events were gated on viable single cells.

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  Flow Cytometry (Intracellular) - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol-permeabilized RAB8A KO HeLa (ED010027, Left) / Parental HeLa (EDWT01, Right) cells labeling RAB8A with Anti-RAB8A antibody [MJF-R22-79-3] - Mouse IgG2a (Chimeric) ab307607 at 1/800 dilution (0.1 ug) (Red) compared with a mouse monoclonal IgG (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-Mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

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  Flow Cytometry - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell) (Left panel) / MCF7 (Human breast adenocarcinoma epithelial cell) (Right panel) cells labeling EpCAM with Anti-EpCAM antibody [AUA1] ab308057 at 1/1000 dilution (0.1ug) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
  
  Negative control: HeLa. (PMID: 31806375).
  
  Gated on viable cells.

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  Flow Cytometry (Intracellular) - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) (Left panel) compared to HDLM-2 (human Hodgkin lymphoma cell) (Right panel) cells labelling Semaphorin 4D/CD100 with Anti-Semaphorin 4D/CD100 antibody [30/CD100] ab307685 at 1/1000 dilution (0.1ug) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-Mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
  
  Negative control: PC-3 (PMID: 23775445).

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  Flow Cytometry - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Flow cytometric analysis of rat bone marrow cells labelling SIRPA with Anti-SIRP alpha antibody [OX-41] ab307563 at 1/1000 dilution (0.1ug) (Right panel) compared with a Mouse monoclonal IgG isotype control (Left panel).
  
  Goat anti-Mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
  
  Gated on viable cells.

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  Flow Cytometry (Intracellular) - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Jurkat (human T cell leukemia T lymphocyte, Left) / Ramos (human Burkitt's lymphoma B lymphocyte, Right) cells labelling Bcl6 with Anti-Bcl6 antibody [K112-91] ab307681 at 1/1000 dilution (0.1ug) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-Mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
  
  Negative control: Jurkat (PMID: 8652841, 10757802).

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  Flow Cytometry (Intracellular) - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized human PBMC (human peripheral blood mononuclear cell) cells labelling CD16 with Anti-CD16 antibody [SP175] - Mouse IgG2a (Chimeric) ab308605 at 1/1000 dilution (0.1 ug)/Right compared with a Mouse monoclonal IgG isotype control/ Left. A Goat anti-Mouse IgG (Alexa Fluor® 488, ab150113) at 1/5000 dilution was used as the secondary antibody. Cells were stained with anti-CD14 conjugated to A647. Fixed with 2% PFA for 10 min followed by intracellularly staining with mouse IgG or Anti-CD16 antibody [SP175] - Mouse IgG2a (Chimeric) ab308605.

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  Flow Cytometry (Intracellular) - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Raji (human Burkitt's lymphoma B lymphocyte) cells labelling CD16 with Anti-CD16 antibody [SP175] - Mouse IgG2a (Chimeric) ab308605 at 1/1000 dilution (0.1 ug)/ (Red) compared with a mouse monoclonal IgG isotype control / Left and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-Mouse IgG (Alexa Fluor® 488, ab150113) at 1/5000 dilution was used as the secondary antibody.
  
  Negative control: Raji. (PMID:11207281).

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  Flow Cytometry - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Flow cytometric analysis of Raji (human Burkitt's lymphoma B lymphocyte, Left) / Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Right) cells labelling CD3 epsilon with Anti-CD3 epsilon antibody [BC3] ab307971 at 1/1000 dilution (0.1 ug)/Red compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-Mouse IgG (Alexa Fluor® 488, ab150113) at 1/5000 dilution was used as the secondary antibody. Negative control: Raji. Gated on viable cells.

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  Flow Cytometry - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

  Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) cells labelling CD3 epsilon with Anti-CD3 epsilon antibody [BC3] ab307971 at 1/1000 dilution (0.1 ug)/Red compared with a Mouse monoclonal IgG / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-Mouse IgG (Alexa Fluor® 488, ab150113) at 1/5000 dilution was used as the secondary antibody.