Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed

29 product images

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)

  ICC/IF image of Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, 10μg/ml) overnight at +4°C. The secondary antibody (red) was ab150119 Alexa Fluor^® 647 goat anti-mouse IgG (H+L) used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
  

  The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

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  Alexa Fluor® - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)
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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)

  GFAP immunofluorescence staining of primary hippocampal mouse neurons/glia using chicken anti-GFAP antibody
  
  Immunofluorescence staining of SOX9 using Anti-SOX9 antibody [EPR14335] ab185230 in primary hippocampal mouse neurons/glia (obtained from Transnetyx Tissue by BrainBits LLC cat.no. C57EHP) DIV14. The cells were fixed with 4% formaldehyde (10 min) permeabilized with 0.1% TritonX-100 (in PBS) for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Anti-SOX9 antibody [EPR14335] ab185230 at 5 μg/ml, Anti-GFAP antibody ab4674 (anti-GFAP) at 1/1000 dilution and Anti-NeuN antibody [1B7] - Neuronal Marker ab104224 (anti-NeuN) at 1/1000 dilution. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (shown in green) Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed ab150176 Goat Anti-Chicken IgY H&L (Alexa Fluor^® 594) preadsorbed (shown in red) and ab150119 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 647) (shown in purple) all secondary antibodies at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue).
  As expected most GFAP positive cells are also SOX9 positive while NeuN positive cells are SOX9 negative.
  Images were acquired with the Perkin Elmer Operetta HCA and a maximum intensity projection of confocal sections is shown.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)

  Vimentin immunofluorescence staining of VIM KO cells using rabbit anti-Vimentin antibody
  
  Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker ab92547 staining VIM in wild-type HeLa cells with negative expression in VIM knockout HeLa cells. The cells were fixed with 4% formaldehyde (10 min) permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker ab92547 at 2 μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 Mouse monoclonal [DM1A] to alpha Tubulin at 0.5 μg/ml. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488) pre-adsorbed at 1/1000 dilution (shown in green) and ab150119 Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor^® 647) pre-adsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.This product also work with 100% methanol (5 min) fixation under the same testing conditions.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)

  Immunofluorescence staining of Tyrosine Hydroxylase in sections of formalin-fixed paraffin-embedded rat brain (positive) and rat liver (negative).
  
  Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with Anti-Tyrosine Hydroxylase antibody [EP1532Y] – Mouse IgG1 (Chimeric) – BSA and Azide Free ab317796 at 1/500 dilution, and then incubated for 1 hour with ab150119 Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)

  Immunofluorescence staining of Histone H3 (acetyl K27) in sections of formalin-fixed paraffin-embedded rat kidney (positive).
  
  Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with Anti-Histone H3 (acetyl K27) antibody [EP16602] – Ms IgG1 (Chimeric) – BSA and Azide Free ab317802 at 1/100 dilution, and then incubated for 1 hour with ab150119 Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)

  Immunofluorescence staining of Histone H3 (acetyl K27) in sections of formalin-fixed paraffin-embedded human colon (positive).
  
  Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with Anti-Histone H3 (acetyl K27) antibody [EP16602] – Ms IgG1 (Chimeric) – BSA and Azide Free ab317802 at 1/100 dilution, and then incubated for 1 hour with ab150119 Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
  
  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)

  Immunofluorescence staining of Histone H3 (tri methyl K9) in sections of formalin-fixed paraffin-embedded human colon*.
  
  Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with Anti-Histone H3 (tri methyl K9) antibody [EPR16601] – Mouse IgG1 (Chimeric) – BSA and Azide Free ab317800 at 1/500 dilution, and then incubated for 1 hour with ab150119 Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
  
  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)

  Immunofluorescence staining of Collagen alpha-1(I) chain in sections of formalin-fixed paraffin-embedded human colon*.
  
  Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with Anti-Collagen I antibody [EPR7785] – Mouse IgG1 (Chimeric) – BSA and Azide Free ab317799 at 1/500 dilution, and then incubated for 1 hour with ab150119 Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
  
  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK293-GFP-LRRK2 cells labelling RAB10 (phospho T73) with Anti-RAB10 (phospho T73) antibody [MJF-R21] - Mouse IgG2a (Chimeric) ab315174 at 1/400 dilution, followed by ab150119 Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119) antibody at 1/1000 2 ug/ml dilution (Green).

  Increased number of strongly stained cytoplasmic foci after Doxycycline treatment compared to untreated cells and after MLi-2 treatment as expected. Images were acquired on the Perkin Elmer® Operetta HCA and a maximum intensity projection of 8 confocal planes is shown for the representative images.

  Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab202272 Recombinant Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab202272) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is ab150119 Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119) at 1/1000 2 ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK293-GFP-LRRK2 cells labelling RAB10 (phospho T73) with Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] - Mouse IgG2a (Chimeric) ab315172 at 1/400 dilution, followed by ab150119 Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119) antibody at 1/1000 2 ug/ml dilution (Green).

  Increased number of strongly stained cytoplasmic foci after Doxycycline treatment compared to untreated cells and after MLi-2 treatment as expected. Images were acquired on the Perkin Elmer® Operetta HCA and a maximum intensity projection of 8 confocal planes is shown for the representative images.

  Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab202272 Recombinant Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab202272) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is ab150119 Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119) at 1/1000 2 ug/ml dilution.

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  Flow Cytometry - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)

  Flow Cytometry analysis of human peripheral blood mononuclear cell (PBMC) treated with 100ng/ml GM-CSF and 50ng/ml IL-4 for 3 days then change new medium (100ng/ml GM-CSF and 50ng/ml IL-4) for another 3 days (right panel) compared to mouse monoclonal IgG isotype control (left panel).
  
  Anti-CD83 antibody [HB15e] ab123494 used at a 1/400 dilution.
  
  Secondary is Goat Anti-Mouse IgG (Alexa Fluor® 647, ab150119) used at a 1/2000 dilution.
  
  Gated on viable cells.
  Bright secondary antibody like Alexa Fluor®647 conjugated ones are recommended to get desirable signal.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)

  Anti-MLH1 antibody [EPR20522] ab223844 staining MLH1 in wild-type Hap1 cells, with negative expression in MLH1 knockout Hap1 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Anti-MLH1 antibody [EPR20522] ab223844 at 0.2 μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin at 0.5 μg/ml. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150119, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647), pre-adsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)

  Anti-PAI1 antibody [EPR21850-82] ab222754 staining SERPINE1 in HUV-EC cells, with negative expression in HEK293 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Anti-PAI1 antibody [EPR21850-82] ab222754 at 0.1 μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin at 0.5 μg/ml. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150119, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647), pre-adsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)

  Anti-DUSP4 antibody [EPR19881] ab216576 staining DUSP4 in wild-type A549 cells, with negative expression in DUSP4 knockout A549 cells. The cells were fixed with 100% methanol (5 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Anti-DUSP4 antibody [EPR19881] ab216576 at 1 μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin at 0.5 μg/ml. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150119, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647), pre-adsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)

  Anti-TSG101 antibody [EPR7130(B)] ab125011 staining TSG101 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Anti-TSG101 antibody [EPR7130(B)] ab125011 at 1 μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin at 0.5 μg/ml. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150119, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647), pre-adsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)

  Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730 staining SLC2A1 in wild-type A549 cells, with negative expression in SLC2A1 knockout A549 cells. The cells were fixed with 100% methanol (5 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Anti-Glucose Transporter GLUT1 antibody [EPR3915] ab115730 at 1 μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin at 0.5 μg/ml. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150119, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647), pre-adsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)

  Anti-ERG antibody [EPR3864] ab92513 staining ERG in THP-1 cells, with negative expression in HCT116 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Anti-ERG antibody [EPR3864] ab92513 at 1 μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin at 0.5 μg/ml. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150119, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647), pre-adsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)

  Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker ab52625 staining KRT19 in MCF7 cells, with negative expression in SHSY5Y cells. The cells were fixed with 100% methanol (5 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker ab52625 at 2 μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin at 0.5 μg/ml. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150119, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647), pre-adsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown. This product also work with 4% formaldehyde (10 min) fixation under the same testing conditions.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells labelling T2A + P2A with Anti-T2A + P2A antibody [3H4] ab269488 at 1/100 (9.29 ug/ml) dilution, followed by ab150119 Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic and nuclear staining in HEK-293T cells transfected with a T2A expression vector containing a GFP tag is observed. The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is ab150119 Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119) at 1/1000 2 ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells labelling T2A + P2A with Anti-T2A + P2A antibody [3H4] ab269488 at 1/100 (9.29 ug/ml) dilution, followed by ab150119 Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic and nuclear staining in HEK-293T cells transfected with a P2A expression vector containing a GFP tag is observed. The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is ab150119 Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119) at 1/1000 2 ug/ml dilution.

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  Flow Cytometry - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)

  Flow Cytometry analysis of HCT 116 (Human colorectal carcinoma epithelial cell, Left) / MCF7 ( Human breast adenocarcinoma epithelial cell, Right) cells stained for MUC1 using Anti-MUC1 antibody [HMFG1 (aka 1.10.F3)] ab70475 at a 1/1000 dilution (0.1 μg) (Red) compared to Mouse monoclonal IgG - Isotype Control (Black) and cells without incubation with primary antibody and secondary antibody (Blue).
  
  Negative control; HCT 116. (PMID: 14998492)

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)

  PE Anti-p53 antibody [E47] ab208992 staining wildtype p53 in Hek293 cells (a high expressing cell line). The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with PE Anti-p53 antibody [E47] ab208992 at 0.2µg/ml (pseudocoloured in green) and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150119, Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
  
  Also suitable in cells fixed with 4% paraformaldehyde (10 min).
  
  Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)

  PE Anti-p53 antibody [E47] ab208992 staining mutant p53 in wild-type Hap1 cells (top panel) and p53 knockout Hap1 cells (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with PE Anti-p53 antibody [E47] ab208992 at 0.2µg/ml (pseudocoloured in green) and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150119, Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
  
  Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)

  PE Anti-p53 antibody [E47] ab208992 staining mutant p53 in A431 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with PE Anti-p53 antibody [E47] ab208992 at 0.2µg/ml (pseudocoloured in green) and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150119, Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
  
  Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)

  Anti-HLA-DR antibody [TAL 1B5] ab20181 staining HLA DR in Raji cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Anti-HLA-DR antibody [TAL 1B5] ab20181 at 1/1000 dilution and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal to alpha Tubulin at 1/1000 dilution. Cells were then incubated with ab150119, Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed, at 1/1000 dilution (shown in red) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488), preadsorbed at 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)

  Immunofluorescence staining of SOX9 using Alexa Fluor® 488 Anti-SOX9 antibody [EPR14335] ab196450 in primary hippocampal mouse neurons/glia (obtained from Transnetyx Tissue by BrainBits LLC cat.no. C57EHP) DIV14. The cells were fixed with 4% formaldehyde (10 min) permeabilized with 0.1% TritonX-100 (in PBS) for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Alexa Fluor® 488 Anti-SOX9 antibody [EPR14335] ab196450 at 1 μg/ml (shown in green), Anti-GFAP antibody ab4674 (anti-GFAP) at 1/1000 dilution and Anti-NeuN antibody [1B7] - Neuronal Marker ab104224 (anti-NeuN) at 1/1000 dilution. Cells were then incubated with Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed ab150176 Goat Anti-Chicken IgY H&L (Alexa Fluor^® 594) preadsorbed (shown in red) and ab150119 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 647) (shown in purple) all secondary antibodies at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue).
  As expected most GFAP positive cells are also SOX9 positive while NeuN positive cells are SOX9 negative.
  Images were acquired with the Perkin Elmer Operetta HCA and a maximum intensity projection of confocal sections is shown.

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  Flow Cytometry (Intracellular) - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized p53 KO HAP1 (human p53 knockout chronic myelogenous leukemia near-haploid cell, Left) / Parental HAP1 (Right) cells labelling p53 with Anti-p53 antibody [G59-12] ab308609 at 1/1000 dilution (0.1 ug) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Mouse IgG (Alexa Fluor® 647, ab150119) at 1/5000 dilution was used as the secondary antibody.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (ab150119)

  Immunofluorescence staining of SOX9 using Anti-SOX9 antibody [EPR14335-78] ab185966 in primary hippocampal mouse neurons/glia, (obtained from Transnetyx Tissue by BrainBits, LLC, cat.no. C57EHP), DIV14. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% TritonX-100 (in PBS) for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Anti-SOX9 antibody [EPR14335-78] ab185966 at 1 μg/ml, Anti-GFAP antibody ab4674 (anti-GFAP) at 1/1000 dilution and Anti-NeuN antibody [1B7] - Neuronal Marker ab104224 (anti-NeuN) at 1/1000 dilution. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green), Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed ab150176, Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (shown in red) and ab150119, Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (shown in purple), all secondary antibodies at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue).
  As expected, most GFAP positive cells are also SOX9 positive, while NeuN positive cells are SOX9 negative. SOX9 positive cells, which are not GFAP positive (e.g. asterisk) are likely neural stem cells/ oligodendrocyte precursor cells present in the culture.
  Images were acquired with the Perkin Elmer Operetta HCA and a maximum intensity projection of confocal sections is shown.