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AB175775

Goat Anti-Mouse IgG H&L (Alexa Fluor® 680)

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(23 Publications)

Suitable for WB. Cited in 23 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

1 Images
Western blot - Goat Anti-Mouse IgG H&L (Alexa Fluor® 680) (AB175775)
  • WB

Lab

Western blot - Goat Anti-Mouse IgG H&L (Alexa Fluor® 680) (AB175775)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab7291 overnight at 4°C. Antibody binding was detected using ab175775 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (<a href='/en-us/products/primary-antibodies/alpha-tubulin-antibody-dm1a-loading-control-ab7291'>ab7291</a>) at 1 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (Alexa Fluor® 680) (ab175775) at 1/10000 dilution

Predicted band size: 36 kDa

Observed band size: 50 kDa

false

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 680

Excitation/Emission

Ex: 679nm, Em: 702nm

Applications

WB

applications

Clonality

Polyclonal

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000", "notes":"<p></p>" } } }

Product details

We batch test Goat Anti-Mouse IgG H&L (Alexa Fluor® 680),ab175775 in fluorescent WB. Although we don't batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success using Goat Anti-Mouse IgG H&L (Alexa Fluor® 680), ab175775 in these applications.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

Target data

See full target information Ighg1

Additional targets

Ighg1

Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Science (New York, N.Y.) 389:eadr5817 PubMed40638752

2025

Cryo-EM structure of human telomerase dimer reveals H/ACA RNP-mediated dimerization.

Applications

Unspecified application

Species

Unspecified reactive species

Sebastian Balch,Zala Sekne,Elsa Franco-Echevarría,Patryk Ludzia,Rachael C Kretsch,Wenqing Sun,Haopeng Yu,George E Ghanim,Sigurdur Thorkelsson,Yiliang Ding,Rhiju Das,Thi Hoang Duong Nguyen

Scientific reports 15:4507 PubMed39915561

2025

Purpurin suppresses Salmonella invasion of host cells by reducing the secretion of T3SS-1 effector proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenxu Shi,Zhimin Guo,Siqi Li,Chenxiao Jiang,Jianfeng Wang,Xuming Deng,Hongtao Liu,Jiazhang Qiu

Proteomes 12: PubMed39728915

2024

Peptidomic Analysis Reveals Temperature-Dependent Proteolysis in Rainbow Trout () Meat During Sous-Vide Cooking.

Applications

Unspecified application

Species

Unspecified reactive species

Miyu Sakuyama,Yuri Kominami,Hideki Ushio

Frontiers in immunology 15:1490776 PubMed39588374

2024

Distinct distribution and responses of IgM, IgT1 and IgT2 B cells in common carp.

Applications

Unspecified application

Species

Unspecified reactive species

Awatif Eltijani,Carmen W E Embregts,Susana Magadan,Jingjing Wang,Sylvia Brugman,Pierre Boudinot,Geert F Wiegertjes,Maria Forlenza

Nature protocols 20:1057-1081 PubMed39438698

2024

High-throughput capture and in situ protein analysis of extracellular vesicles by chemical probe-based array.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Feng,Ao Shen,Wei Zhang,Shengnan Jia,Anton Iliuk,Yuling Wang,Wenke Zhang,Ying Zhang,W Andy Tao,Lianghai Hu

Nature communications 15:746 PubMed38272871

2024

2.7 Å cryo-EM structure of human telomerase H/ACA ribonucleoprotein.

Applications

Unspecified application

Species

Unspecified reactive species

George E Ghanim,Zala Sekne,Sebastian Balch,Anne-Marie M van Roon,Thi Hoang Duong Nguyen

Zoological research 45:13-24 PubMed38114429

2023

Full T-cell activation and function in teleosts require collaboration of first and co-stimulatory signals.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Liang,Kang Li,Haiyou Gao,Kunming Li,Jiansong Zhang,Qian Zhang,Xinying Jiao,Jialong Yang,Xiumei Wei

Carbohydrate polymers 320:121214 PubMed37659814

2023

Low-molecular weight sulfated marine polysaccharides: Promising molecules to prevent neurodegeneration in mucopolysaccharidosis IIIA?

Applications

Unspecified application

Species

Unspecified reactive species

Noemi Veraldi,Isabelle Dentand Quadri,Yohan van de Looij,Laura Malaguti Modernell,Corinne Sinquin,Agata Zykwinska,Benjamin B Tournier,Fabien Dalonneau,Honglian Li,Jin-Ping Li,Philippe Millet,Romain Vives,Sylvia Colliec-Jouault,Ariane de Agostini,Eduardo Farias Sanches,Stéphane V Sizonenko

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2201164 PubMed36890649

2023

Glutamine Metabolism Underlies the Functional Similarity of T Cells between Nile Tilapia and Tetrapod.

Applications

Unspecified application

Species

Unspecified reactive species

Kang Li,Xiumei Wei,Xinying Jiao,Wenhai Deng,Jiaqi Li,Wei Liang,Yu Zhang,Jialong Yang

Microbiology spectrum 10:e0294922 PubMed36377917

2022

Inhibition of the Type III Secretion System of Salmonella enterica Serovar Typhimurium via Treatment with Fraxetin.

Applications

Unspecified application

Species

Unspecified reactive species

Yunjia Shi,Zeyu Sun,Yang Liu,Jingyan Shu,Yong Zhang,Qianghua Lv,Jianfeng Wang,Xuming Deng,Hongtao Liu,Jiazhang Qiu
View all publications

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