Goat Anti-Mouse IgG H&L (Alexa Fluor® 680) preadsorbed
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(6 Publications)
Suitable for WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 6 publications.
View Alternative Names
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form
- WB
Lab
Western blot - Goat Anti-Mouse IgG H&L (Alexa Fluor® 680) preadsorbed (AB186694)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab7291 overnight at 4°C. Antibody binding was detected using ab186694 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes:
Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (<a href='/en-us/products/primary-antibodies/alpha-tubulin-antibody-dm1a-loading-control-ab7291'>ab7291</a>) at 1 µg/mL
All lanes:
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Mouse IgG H&L (Alexa Fluor® 680) preadsorbed (ab186694) at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 50 kDa
false
Reactivity data
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Why is this recommended?
We recommend this product because it’s often used in the same experiment or related research.
We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.
Product details
We batch test Goat Anti-Mouse IgG H&L (Alexa Fluor® 680) preadsorbed, ab186694 in fluorescent WB. Although we don't batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success using Goat Anti-Mouse IgG H&L (Alexa Fluor® 680) preadsorbed, ab186694 in these applications.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties and storage information
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Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
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Publications (6)
Recent publications for all applications. Explore the full list and refine your search
Communications biology 6:226 PubMed36854740
2023
Applications
Unspecified application
Species
Unspecified reactive species
ACS omega 6:17276-17288 PubMed34278114
2021
Applications
Unspecified application
Species
Unspecified reactive species
eLife 10: PubMed33835029
2021
Applications
Unspecified application
Species
Unspecified reactive species
Scientific reports 10:2040 PubMed32029775
2020
Applications
Unspecified application
Species
Unspecified reactive species
American journal of physiology. Endocrinology and metabolism 316:E73-E85 PubMed30422704
2018
Applications
Unspecified application
Species
Unspecified reactive species
Molecular medicine reports 12:3095-100 PubMed25955232
2015
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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