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AB175741

Goat Anti-Mouse IgG H&L (Alexa Fluor® 750) preadsorbed

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(2 Publications)

Suitable for WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form

1 Images
Western blot - Goat Anti-Mouse IgG H&L (Alexa Fluor® 750) preadsorbed (AB175741)
  • WB

Lab

Western blot - Goat Anti-Mouse IgG H&L (Alexa Fluor® 750) preadsorbed (AB175741)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab7291 overnight at 4°C. Antibody binding was detected using ab175741 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (<a href='/en-us/products/primary-antibodies/alpha-tubulin-antibody-dm1a-loading-control-ab7291'>ab7291</a>) at 1 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (Alexa Fluor® 750) preadsorbed (ab175741) at 1/10000 dilution

Predicted band size: 36 kDa

Observed band size: 50 kDa

false

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rabbit, Horse, Chicken, Cow, Human, Pig, Rat

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 750

Excitation/Emission

Ex: 749nm, Em: 775nm

Applications

WB

applications

Clonality

Polyclonal

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, horse, human, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000", "notes":"<p></p>" } } }

Product details

We batch test Goat Anti-Mouse IgG H&L (Alexa Fluor® 750) preadsorbed, ab175741 in fluorescent WB. Although we don't batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success usingGoat Anti-Mouse IgG H&L (Alexa Fluor® 750) preadsorbed, ab175741 in these applications.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

Target data

See full target information Ighg1

Additional targets

Ighg1

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Free radical biology & medicine 204:252-265 PubMed37192685

2023

Performance benchmarking microplate-immunoassays for quantifying target-specific cysteine oxidation reveals their potential for understanding redox-regulation and oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmet Tuncay,Daniel R Crabtree,David J Muggeridge,Holger Husi,James N Cobley

Redox biology 26:101258 PubMed31234016

2019

Catalyst-free Click PEGylation reveals substantial mitochondrial ATP synthase sub-unit alpha oxidation before and after fertilisation.

Applications

Unspecified application

Species

Unspecified reactive species

James N Cobley,Anna Noble,Eduardo Jimenez-Fernandez,Manuel-Thomas Valdivia Moya,Matthew Guille,Holger Husi
View all publications

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