Goat Anti-Mouse IgG H&L (Alexa Fluor® 790)

4 Images

• WB

Lab

Western blot - Goat Anti-Mouse IgG H&L (Alexa Fluor® 790) (AB175783)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab8224 overnight at 4°C. Antibody binding was detected using a goat anti-mouse Alexa Fluor 790 (ab175783) at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-beta Actin antibody [mAbcam 8224] - Loading Control (<a href='/en-us/products/primary-antibodies/beta-actin-antibody-mabcam-8224-loading-control-ab8224'>ab8224</a>) at 1 µg/mL

Lane 1:

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg

Lane 3:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg

Lane 4:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 20 µg

Lane 5:

Skeletal Muscle (Human) Tissue Lysate - adult normal tissue at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (Alexa Fluor® 790) (ab175783) at 1/10000 dilution

Predicted band size: 41 kDa

Observed band size: 42 kDa

false

• WB

Unknown

Western blot - Goat Anti-Mouse IgG H&L (Alexa Fluor® 790) (AB175783)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab8226 overnight at 4°C. Antibody binding was detected using Goat Anti-Mouse IgG H&L (Alexa Fluor® 790) (ab175783) at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-beta Actin antibody [mAbcam 8226] - Loading Control (<a href='/en-us/products/primary-antibodies/beta-actin-antibody-mabcam-8226-loading-control-ab8226'>ab8226</a>) at 1/1000 dilution

Lane 1:

A431 (Human epidermoid carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg

Lane 3:

NIH 3T3 (Mouse embryo fibroblast cell line) Whole Cell Lysate at 20 µg

Lane 4:

PC12 (Rat adrenal gland pheochromocytoma cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (Alexa Fluor® 790) (ab175783) at 1/10000 dilution

Predicted band size: 36 kDa,41 kDa

Observed band size: 42 kDa

false

• WB

Unknown

Western blot - Goat Anti-Mouse IgG H&L (Alexa Fluor® 790) (AB175783)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab7291 overnight at 4°C. Antibody binding was detected using Anti-Mouse Alexa Fluor® 790 (ab175783) at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (<a href='/en-us/products/primary-antibodies/alpha-tubulin-antibody-dm1a-loading-control-ab7291'>ab7291</a>) at 1/5000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) Whole Cell Lysate at 20 µg

Lane 2:

HEK293 (Human epithelial cell line from embryonic kidney) Whole Cell Lysate at 20 µg

Lane 3:

HepG2 (Human liver hepatocellular carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 4:

Caco 2 (Human colorectal adenocarcinoma cell line) Whole Cell Lysate at 20 µg

Lane 5:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg

Lane 6:

PC12 (Rat adrenal gland pheochromocytoma cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (Alexa Fluor® 790) (ab175783) at 1/10000 dilution

Predicted band size: 36 kDa

false

• WB

Lab

Western blot - Goat Anti-Mouse IgG H&L (Alexa Fluor® 790) (AB175783)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab7291 overnight at 4°C. Antibody binding was detected using ab175783 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (<a href='/en-us/products/primary-antibodies/alpha-tubulin-antibody-dm1a-loading-control-ab7291'>ab7291</a>) at 1 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (Alexa Fluor® 790) (ab175783) at 1/10000 dilution

Predicted band size: 36 kDa

Observed band size: 50 kDa

false