Suitable for WB. Cited in 3 publications.
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- BMC cancer 22:3082022MiR-106b-5p regulates esophageal squamous cell carcinoma progression by binding to HPGD.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFan Yang,Zhanwen Sun,Dengyun Wang,Tian DuPubMed 35317779
- Oncology letters 22:6082021MicroRNA-744-5p inhibits glioblastoma malignancy by suppressing replication factor C subunit 2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFei Fan,Dongxiao Yao,Pengfei Yan,Xiaobing Jiang,Jie HuPubMed 34188710
- Oncology letters 21:1612021MicroRNA-640 promotes cell proliferation and adhesion in glioblastoma by targeting Slit guidance ligand 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Luo,Zhiying Lu,Yongli Chen,Xiaozhen Chen,Na Liu,Jing Chen,Shanwu DongPubMed 33552279
Key facts
- Host species
- Goat
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 790
- Excitation/Emission
- Ex: 782nm, Em: 805nm
- Applications
- WB
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info 1/10000 | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Additional Targets
Alternative names
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form
Recommended products
Suitable for WB. Cited in 3 publications.
Key facts
- Description
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 790)
- Host species
- Goat
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 790
- Excitation/Emission
- Ex: 782nm, Em: 805nm
- Applications
- WB
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
We batch test Goat Anti-Mouse IgG H&L (Alexa Fluor® 790), ab175783 in fluorescent WB. Although we don't batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success using Goat Anti-Mouse IgG H&L (Alexa Fluor® 790), ab175783 in these applications.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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4 product images
Western blot - Goat Anti-Mouse IgG H&L (Alexa Fluor® 790) (ab175783)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226 overnight at 4°C. Antibody binding was detected using Goat Anti-Mouse IgG H&L (Alexa Fluor® 790) (ab175783) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes: Western blot - Anti-beta Actin antibody [mAbcam 8226] - Loading Control (Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226) at 1/1000 dilution
Lane 1: A431 (Human epidermoid carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg
Lane 3: NIH 3T3 (Mouse embryo fibroblast cell line) Whole Cell Lysate at 20 µg
Lane 4: PC12 (Rat adrenal gland pheochromocytoma cell line) Whole Cell Lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (Alexa Fluor® 790) (ab175783) at 1/10000 dilution
Predicted band size: 36 kDa, 41 kDa
Observed band size: 42 kDa
Western blot - Goat Anti-Mouse IgG H&L (Alexa Fluor® 790) (ab175783)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with Anti-beta Actin antibody [mAbcam 8224] - Loading Control ab8224 overnight at 4°C. Antibody binding was detected using a goat anti-mouse Alexa Fluor 790 (ab175783) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes: Western blot - Anti-beta Actin antibody [mAbcam 8224] - Loading Control (Anti-beta Actin antibody [mAbcam 8224] - Loading Control ab8224) at 1 µg/mL
Lane 1: A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg
Lane 3: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg
Lane 4: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 20 µg
Lane 5: Skeletal Muscle (Human) Tissue Lysate - adult normal tissue at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (Alexa Fluor® 790) (ab175783) at 1/10000 dilution
Predicted band size: 41 kDa
Observed band size: 42 kDa
Western blot - Goat Anti-Mouse IgG H&L (Alexa Fluor® 790) (ab175783)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 overnight at 4°C. Antibody binding was detected using Anti-Mouse Alexa Fluor® 790 (ab175783) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes: Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/5000 dilution
Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) Whole Cell Lysate at 20 µg
Lane 2: HEK293 (Human epithelial cell line from embryonic kidney) Whole Cell Lysate at 20 µg
Lane 3: HepG2 (Human liver hepatocellular carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 4: Caco 2 (Human colorectal adenocarcinoma cell line) Whole Cell Lysate at 20 µg
Lane 5: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg
Lane 6: PC12 (Rat adrenal gland pheochromocytoma cell line) Whole Cell Lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (Alexa Fluor® 790) (ab175783) at 1/10000 dilution
Predicted band size: 36 kDa
Western blot - Goat Anti-Mouse IgG H&L (Alexa Fluor® 790) (ab175783)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 overnight at 4°C. Antibody binding was detected using ab175783 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes: Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1 µg/mL
All lanes: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (Alexa Fluor® 790) (ab175783) at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 50 kDa
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