AB97020

Goat Anti-Mouse IgG H&L (Alkaline Phosphatase)

Name: Goat Anti Mouse (IgG) secondary antibody AP (ab97020) | Abcam
Brand: Abcam Limited
SKU: AB97020
Price: 265 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(33 Publications)

Suitable for ICC, WB, IHC-P, ELISA. Cited in 33 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

1 Images

Unknown

Western blot - Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (AB97020)

All lanes:

Western blot - Anti-beta Actin antibody [mAbcam 8226] - Loading Control (<a href='/en-us/products/primary-antibodies/beta-actin-antibody-mabcam-8226-loading-control-ab8226'>ab8226</a>) at 1 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97020) at 1/5000 dilution

Lanes 3 - 4:

Western blot - Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97020) at 1/20000 dilution

Lanes 5 - 6:

Western blot - Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97020) at 1/50000 dilution

Predicted band size: 36 kDa

true

Exposure time: 4min

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Alkaline Phosphatase

Excitation/Emission

Applications

ELISA, WB, IHC-P, ICC

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/5000 - 1/50000", "notes":"(Chemiluminescent)" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/1000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/5000 - 1/50000", "notes":"(Primary ELISA)" } } }

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Product details

Part of the AbExcel range.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA, 0.0095% Magnesium chloride, 0.00136% Zinc chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (33)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:29353 PubMed40790053

2025

Tat-specific antibodies associated with better HIV-associated motor function.

Applications

Unspecified application

Species

Unspecified reactive species

Catarina E Hioe,Clauvis Kunkeng Yengo,Xiaomei Liu,Reuben Davis,Gospel Enyindah-Asonye,Jéromine Klingler,Alex F Tang,Juan C Bandres,Anuradha Ganesan,Tahaniyat Lalani,Joseph Yabes,Brian K Agan,Xiaokun Liu,David J Volsky,Susan Morgello,Jessica Robinson-Papp

Clinical proteomics 22:16 PubMed40295933

2025

An ALS assembly modulator signature in peripheral blood mononuclear cells: implications for ALS pathophysiology, therapeutics, and diagnostics.

Applications

Unspecified application

Species

Unspecified reactive species

Shao Feng Yu,Maya Michon,Anuradha F Lingappa,Kumar Paulvannan,Dennis Solas,Kim Staats,Justin Ichida,Debendranath Dey,Jeffrey Rosenfeld,Vishwanath R Lingappa

Inflammation research : official journal of the European Histamine Research Society ... [et al.] 74:68 PubMed40272538

2025

Eosinophils but not mast cells exert anti-tumorigenic activity, without being predictive markers of the long-term response to Bacillus Calmette-Guérin (BCG) therapy in patients with bladder carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ilan Zaffran,Yara Zoabi,Pratibha Gaur,Fidan Rahimli Alekberli,Ekaterini Tiligada,Vladimir Yutkin,Francesca Levi-Schaffer

Molecular ecology 33:e17534 PubMed39314079

2024

Interplay between vertebrate adaptive immunity and bacterial infectivity genes: Bank vole MHC versus Borrelia afzelii OspC.

Applications

Unspecified application

Species

Unspecified reactive species

Joanna Różańska-Wróbel,Magdalena Migalska,Anna Urbanowicz,Maciej Grzybek,Ryan O M Rego,Anna Bajer,Dorota Dwuznik-Szarek,Mohammed Alsarraf,Jolanta Behnke-Borowczyk,Jerzy M Behnke,Jacek Radwan

Frontiers in pharmacology 14:1335305 PubMed38235110

2024

Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Uma Maheswara Rao Dindi,Sameer Al-Ghamdi,Naif Abdurhman Alrudian,Salman Bin Dayel,Abdulwahab Ali Abuderman,Mohammed Saad Alqahtani,Nasraddin Othman Bahakim,Thiyagarajan Ramesh,Ravikumar Vilwanathan

Cancers 15: PubMed38067256

2023

Influence of C Nanofilm on the Expression of Selected Markers of Mesenchymal-Epithelial Transition in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Malwina Sosnowska,Marta Kutwin,Katarzyna Zawadzka,Michał Pruchniewski,Barbara Strojny,Zuzanna Bujalska,Mateusz Wierzbicki,Sławomir Jaworski,Ewa Sawosz

Blood 142:1918-1927 PubMed37774369

2023

Cold exposure induces vaso-occlusion and pain in sickle mice that depend on complement activation.

Applications

Unspecified application

Species

Unspecified reactive species

Zalaya K Ivy,John D Belcher,Iryna A Khasabova,Chunsheng Chen,Joseph P Juliette,Fuad Abdulla,Conglin Ruan,Kaje Allen,Julia Nguyen,Victoria M Rogness,Joan D Beckman,Sergey G Khasabov,Kalpna Gupta,Ronald P Taylor,Donald A Simone,Gregory M Vercellotti

Journal of nanobiotechnology 21:175 PubMed37264420

2023

A liposomal carbohydrate vaccine, adjuvanted with an NKT cell agonist, induces rapid and enhanced immune responses and antibody class switching.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Xiang Jia,Sen-Lin Peng,Ndayambaje Yvan Kalisa,Qiang Chao,Zhifang Zhou,Xiao-Dong Gao,Ning Wang

Frontiers in immunology 14:1143034 PubMed37063887

2023

Development of a dual vaccine against East Coast fever and lumpy skin disease.

Applications

Unspecified application

Species

Unspecified reactive species

Leah Whittle,Ros Chapman,Nicola Douglass,Mohamed Jaffer,Emmanuel Margolin,Edward Rybicki,Anna-Lise Williamson

Scientific reports 13:4036 PubMed36899011

2023

Novel long non-coding RNAs associated with inflammation and macrophage activation in human.

Applications

Unspecified application

Species

Unspecified reactive species

Avisankar Chini,Prarthana Guha,Venkat S Malladi,Zibiao Guo,Subhrangsu S Mandal

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat Anti-Mouse IgG H&L (Alkaline Phosphatase)

Western blot - Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (AB97020)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Additional targets

Publications (33)

Product promise