Suitable for ICC, WB, IHC-P, ELISA. Cited in 28 publications.
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- Frontiers in pharmacology 14:13353052024Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesUma Maheswara Rao Dindi,Sameer Al-Ghamdi,Naif Abdurhman Alrudian,Salman Bin Dayel,Abdulwahab Ali Abuderman,Mohammed Saad Alqahtani,Nasraddin Othman Bahakim,Thiyagarajan Ramesh,Ravikumar VilwanathanPubMed 38235110
- Journal of nanobiotechnology 21:1752023A liposomal carbohydrate vaccine, adjuvanted with an NKT cell agonist, induces rapid and enhanced immune responses and antibody class switching.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJi-Xiang Jia,Sen-Lin Peng,Ndayambaje Yvan Kalisa,Qiang Chao,Zhifang Zhou,Xiao-Dong Gao,Ning WangPubMed 37264420
- Frontiers in immunology 14:11430342023Development of a dual vaccine against East Coast fever and lumpy skin disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeah Whittle,Ros Chapman,Nicola Douglass,Mohamed Jaffer,Emmanuel Margolin,Edward Rybicki,Anna-Lise WilliamsonPubMed 37063887
- Scientific reports 13:40362023Novel long non-coding RNAs associated with inflammation and macrophage activation in human.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAvisankar Chini,Prarthana Guha,Venkat S Malladi,Zibiao Guo,Subhrangsu S MandalPubMed 36899011
- Stem cells international 2022:47754452022Force-Induced Nitric Oxide Promotes Osteogenic Activity during Orthodontic Tooth Movement in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuqing Sun,Jingfei Fu,Feiran Lin,Shengnan Li,Juan Du,Yi Liu,Yuxing BaiPubMed 36110889
- The Plant journal : for cell and molecular biology 110:548-5612022Identification of the genome-wide distribution of cyanobacterial group-2 sigma factor SigE, accountable for its regulon.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRyo Kariyazono,Takashi OsanaiPubMed 35092706
- Vaccines 10:2022Characterization of a Novel Chimeric p67 Antigen Which Incorporates into Virus-like Particles and Is Highly Immunogenic in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeah Whittle,Ros Chapman,Michiel van Diepen,Edward P Rybicki,Anna-Lise WilliamsonPubMed 35214669
- PloS one 16:e02568162021Characterization of linear epitope specificity of antibodies potentially contributing to spontaneous clearance of hepatitis C virus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAsma Ahsan,Saira Dar,Fareeha Hassan,Farkhanda Ghafoor,Muhammad Haroon Yousuf,Syed Shahzad-Ul-HussanPubMed 34449828
- Plant physiology 186:2003-20202021mRNA surveillance complex PELOTA-HBS1 regulates phosphoinositide-dependent protein kinase1 and plant growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Kong,Shutang Tan,Qing Zhao,De-Li Lin,Zhi-Hong Xu,Jiří Friml,Hong-Wei XuePubMed 33930167
Key facts
- Host species
- Goat
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alkaline Phosphatase
- Applications
- ICC, WB, IHC-P, ELISA
- Clonality
- Polyclonal
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/5000 - 1/50000 | Notes (Chemiluminescent) |
Application IHC-P | Reactivity Reacts | Dilution info 1/100 - 1/1000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/5000 - 1/50000 | Notes (Primary ELISA) |
Associated Products
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6 products for Alternative Product
Target data
Additional Targets
Alternative names
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form
Recommended products
Suitable for ICC, WB, IHC-P, ELISA. Cited in 28 publications.
Key facts
- Description
- Goat Anti-Mouse IgG H&L (Alkaline Phosphatase)
- Host species
- Goat
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alkaline Phosphatase
- Applications
- ICC, WB, IHC-P, ELISA
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA, 0.0095% Magnesium chloride, 0.00136% Zinc chloride- Purification technique
- Affinity purification Immunogen
- Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Part of the AbExcel range.
Product promise
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Full details and terms and conditions can be found here:
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1 product image
Western blot - Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97020)
All lanes: Western blot - Anti-beta Actin antibody [mAbcam 8226] - Loading Control (Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226) at 1 µg/mL
All lanes: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
SecondaryLanes 1 - 2: Western blot - Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97020) at 1/5000 dilution
Lanes 3 - 4: Western blot - Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97020) at 1/20000 dilution
Lanes 5 - 6: Western blot - Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab97020) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Exposure time: 4min
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