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Suitable for WB, ICC, IHC-Fr, IHC-P, ELISA.
Alternative names=Ig gamma-1 chain C region secreted form, Ighg1, Igh-4
- Host species
Goat
- Target species
Mouse
- Target isotype
IgG
- Target specificity
Heavy & Light chains
- Minimal cross-reactivity
Human
- Conjugation
Beta-galactosidase
- Applications
WB, ICC, IHC-Fr, IHC-P, ELISA
- Clonality
Polyclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information. Database link: P01868
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application ICC | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Recommended products
Suitable for WB, ICC, IHC-Fr, IHC-P, ELISA.
Alternative names=Ig gamma-1 chain C region secreted form, Ighg1, Igh-4
Key facts
- Description
Goat Anti-Mouse IgG H&L (beta-galactosidase)
- Host species
Goat
- Target species
Mouse
- Target isotype
IgG
- Target specificity
Heavy & Light chains
- Minimal cross-reactivity
Human
- Conjugation
Beta-galactosidase
- Applications
WB, ICC, IHC-Fr, IHC-P, ELISA
- Clonality
Polyclonal
- Pre-adsorbed
No
- Immunogen
The exact immunogen used to generate this antibody is proprietary information. Database link: P01868
- Specificity
ab136775 does not cross react with Human IgG, A, M.
Beta-galactosidase labelling provides good sensitivity and does not present endogenous activity in mammalian cells. It is well adapted to ELISA and produces no or very little background. It is more difficult to use in immunochemistry where its use can be justified only when the endogenous activities of other available labels produce significant problems.- Isotype
IgG
- Concentration
Properties
- Form
Liquid
- Storage buffer
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS
- Purification technique
Affinity purification Immunogen
- Purification notes
ab136775 has been purified using a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography).
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
Preparation of Substrate Solutions: 0.1M phosphate buffer pH7, 1mM MgSO4, 0.2mM MnSO4, 2mM Mg titriplex. Add extemporaneously 7µl/ml beta-mercaptoethanol and 0.9mg/ml ortho-nitrophenyl-beta-galactopyranoside. Incubate for 30 minutes at 37°C. Stop the reaction with V/2 volume of 1M Na2CO3
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