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AB136775

Goat Anti-Mouse IgG H&L (beta-galactosidase)

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(2 Publications)

Suitable for WB, ICC, IHC-Fr, IHC-P, ELISA. Cited in 2 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Human

Pre-adsorbed

No

Conjugation

Beta-galactosidase

Excitation/Emission
Applications

WB, IHC-P, ICC, IHC-Fr, ELISA

applications

Clonality

Polyclonal

Isotype

IgG

Immunogen

Full Length Protein corresponding to Mouse Ighg1. The exact immunogen used to generate this antibody is proprietary information.

P01868

Specificity

ab136775 does not cross react with Human IgG, A, M.
Beta-galactosidase labelling provides good sensitivity and does not present endogenous activity in mammalian cells. It is well adapted to ELISA and produces no or very little background. It is more difficult to use in immunochemistry where its use can be justified only when the endogenous activities of other available labels produce significant problems.

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Alternative Products

Secondary Antibodies

AB150115

Goat Anti-Mouse IgG H&L (Alexa Fluor® 647)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (AB150115)

  • 5
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Secondary Antibodies

AB175473

Goat Anti-Mouse IgG H&L (Alexa Fluor® 568)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 568) (AB175473)

  • 5
  • 1
Secondary Antibodies

AB6785

Goat Anti-Mouse IgG H&L (FITC)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (FITC) (AB6785)

  • 5
  • 1
Secondary Antibodies

AB150116

Goat Anti-Mouse IgG H&L (Alexa Fluor® 594)

Flow Cytometry - Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (AB150116)

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Secondary Antibodies

AB205719

Goat Anti-Mouse IgG H&L (HRP)

Western blot - Goat Anti-Mouse IgG H&L (HRP) (AB205719)

  • 5
  • 4
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/1000", "notes":"<p></p>" } } }
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Product details

Preparation of Substrate Solutions: 0.1M phosphate buffer pH7, 1mM MgSO4, 0.2mM MnSO4, 2mM Mg titriplex. Add extemporaneously 7µl/ml beta-mercaptoethanol and 0.9mg/ml ortho-nitrophenyl-beta-galactopyranoside. Incubate for 30 minutes at 37°C. Stop the reaction with V/2 volume of 1M Na2CO3
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab136775 has been purified using a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography).
Storage buffer
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Avoid freeze / thaw cycle
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Product protocols

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Target data

See full target information Ighg1

Additional targets

Ighg1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:6541 PubMed37848408

2023

Capturing nascent extracellular vesicles by metabolic glycan labeling-assisted microfluidics.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuyue Wu,Wencheng Wang,Chi Zhang,Zhenlong You,Yinyan Zeng,Yinzhu Lu,Suhui Zhang,Xingrui Li,Chaoyong Yang,Yanling Song

Histochemistry and cell biology 159:233-246 PubMed36374321

2022

Galactosidase-catalyzed fluorescence amplification method (GAFAM): sensitive fluorescent immunohistochemistry using novel fluorogenic β-galactosidase substrates and its application in multiplex immunostaining.

Applications

Unspecified application

Species

Unspecified reactive species

Masahiro Hirata,Toshiaki Kogame,Souichi Adachi,Hironori Haga
View all publications
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Product promise

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