AB97021

Goat Anti-Mouse IgG H&L (Biotin)

Name: Goat Anti Mouse (IgG) secondary antibody Biotin (ab97021) | Abcam
Brand: Abcam Limited
SKU: AB97021

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(15 Publications)

Suitable for ICC/IF, ELISA, IHC-P, WB. Cited in 15 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Biotin

Excitation/Emission

Applications

ELISA, IHC-P, WB, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20000 - 1/400000", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500 - 1/5000", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20000 - 1/400000", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed36810131

2023

Skeletal dysplasia-causing TRPV4 mutations suppress the hypertrophic differentiation of human iPSC-derived chondrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda R Dicks,Grigory I Maksaev,Zainab Harissa,Alireza Savadipour,Ruhang Tang,Nancy Steward,Wolfgang Liedtke,Colin G Nichols,Chia-Lung Wu,Farshid Guilak

Methods in molecular biology (Clifton, N.J.) 2598:87-114 PubMed36355287

2022

Chondrogenic Differentiation of Human-Induced Pluripotent Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda R Dicks,Nancy Steward,Farshid Guilak,Chia-Lung Wu

International journal of molecular sciences 22: PubMed34575895

2021

Unveiling the Metabolic Effects of Glycomacropeptide.

Applications

Unspecified application

Species

Unspecified reactive species

Maria João Pena,Raquel Costa,Ilda Rodrigues,Sandra Martins,João Tiago Guimarães,Ana Faria,Conceição Calhau,Júlio César Rocha,Nuno Borges

Annals of biomedical engineering 48:968-979 PubMed31147805

2019

Re-Differentiation of Human Meniscus Fibrochondrocytes Differs in Three-Dimensional Cell Aggregates and Decellularized Human Meniscus Matrix Scaffolds.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Liang,Alexander R A Szojka,Enaam Idrees,Melanie Kunze,Aillette Mulet-Sierra,Adetola B Adesida

Stem cells (Dayton, Ohio) 37:65-76 PubMed30378731

2018

Step-Wise Chondrogenesis of Human Induced Pluripotent Stem Cells and Purification Via a Reporter Allele Generated by CRISPR-Cas9 Genome Editing.

Applications

Unspecified application

Species

Unspecified reactive species

Shaunak S Adkar,Chia-Lung Wu,Vincent P Willard,Amanda Dicks,Adarsh Ettyreddy,Nancy Steward,Nidhi Bhutani,Charles A Gersbach,Farshid Guilak

Tissue engineering. Part A 24:1531-1544 PubMed29756533

2018

Genetic Engineering of Mesenchymal Stem Cells for Differential Matrix Deposition on 3D Woven Scaffolds.

Applications

Unspecified application

Species

Unspecified reactive species

Nguyen P T Huynh,Jonathan M Brunger,Catherine C Gloss,Franklin T Moutos,Charles A Gersbach,Farshid Guilak

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33:358-372 PubMed29985644

2018

High-depth transcriptomic profiling reveals the temporal gene signature of human mesenchymal stem cells during chondrogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Nguyen P T Huynh,Bo Zhang,Farshid Guilak

Biomaterials 91:57-72 PubMed26999455

2016

Fabrication of anatomically-shaped cartilage constructs using decellularized cartilage-derived matrix scaffolds.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher R Rowland,Lina A Colucci,Farshid Guilak

Tissue engineering. Part A 21:1261-74 PubMed25517798

2015

Knockdown of the cell cycle inhibitor p21 enhances cartilage formation by induced pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Brian O Diekman,Pratiksha I Thakore,Shannon K O'Connor,Vincent P Willard,Jonathan M Brunger,Nicolas Christoforou,Kam W Leong,Charles A Gersbach,Farshid Guilak

Arthritis & rheumatology (Hoboken, N.J.) 66:3062-72 PubMed25047145

2014

Use of cartilage derived from murine induced pluripotent stem cells for osteoarthritis drug screening.

Applications

Unspecified application

Species

Unspecified reactive species

Vincent P Willard,Brian O Diekman,Johannah Sanchez-Adams,Nicolas Christoforou,Kam W Leong,Farshid Guilak

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Goat Anti-Mouse IgG H&L (Biotin)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (15)

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