AB97033

Goat Anti-Mouse IgG H&L (Biotin) preadsorbed

Name: Goat Anti Mouse (IgG) secondary antibody preadsorbed Biotin (ab97033) | Abcam
Brand: Abcam Limited
SKU: AB97033
Price: 295 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(8 Publications)

Suitable for WB, ELISA, IHC-P, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 8 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Sheep, Rabbit, Goat, Horse, Chicken, Cow, Human

Pre-adsorbed

Yes

Conjugation

Biotin

Excitation/Emission

Applications

WB, IHC-P, ICC/IF, ELISA

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/200000", "notes":"(Chemiluminescent)." }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/200000", "notes":"(Primary)." }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/250 - 1/2500", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/500", "notes":"" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information Ighg1

Additional targets

Ighg1

style

left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Brain & spine 4:102813 PubMed38681174

2024

A 72-h sedated porcine model of traumatic spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Mathias Møller Thygesen,Seyar Entezari,Nanna Houlind,Teresa Haugaard Nielsen,Nicholas Østergaard Olsen,Tim Damgaard Nielsen,Mathias Skov,Joel Borgstedt-Bendixen,Alp Tankisi,Mads Rasmussen,Halldór Bjarki Einarsson,Peter Agger,Dariusz Orlowski,Stig Eric Dyrskog,Line Thorup,Michael Pedersen,Mikkel Mylius Rasmussen

Acta neuropathologica 146:685-705 PubMed37740734

2023

Transcriptional mutagenesis of α-synuclein caused by DNA oxidation in Parkinson's disease pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Sambuddha Basu,Minkyung Song,Levi Adams,Inhye Jeong,Goun Je,Subhrangshu Guhathakurta,Jennifer Jiang,Nikpreet Boparai,Wei Dai,Fernando Cardozo-Pelaez,Suren A Tatulian,Kyu Young Han,Jordan Elliott,Jean Baum,Pamela J McLean,Dennis W Dickson,Yoon-Seong Kim

The Journal of experimental medicine 220: PubMed36469302

2022

Arachnoid granulations are lymphatic conduits that communicate with bone marrow and dura-arachnoid stroma.

Applications

Unspecified application

Species

Unspecified reactive species

Trishna Shah,Sue E Leurgans,Rashi I Mehta,Jingyun Yang,Chad A Galloway,Karen L de Mesy Bentley,Julie A Schneider,Rupal I Mehta

Advanced healthcare materials 10:e2101370 PubMed34605223

2021

A Modular Biomaterial Scaffold-Based Vaccine Elicits Durable Adaptive Immunity to Subunit SARS-CoV-2 Antigens.

Applications

Unspecified application

Species

Unspecified reactive species

Fernanda Langellotto,Maxence O Dellacherie,Chyenne Yeager,Hamza Ijaz,Jingyou Yu,Chi-An Cheng,Nikolaos Dimitrakakis,Benjamin T Seiler,Makda S Gebre,Tal Gilboa,Rebecca Johnson,Nadia Storm,Sarai Bardales,Amanda Graveline,Des White,Christina M Tringides,Mark J Cartwright,Edward J Doherty,Anna Honko,Anthony Griffiths,Dan H Barouch,David R Walt,David J Mooney

Translational cancer research 9:1215-1224 PubMed35117466

2020

Clinicopathological significance and prognostic implication of CD44 and its splice variants (v3 and v6) in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Yan,Ying Mu,Meiqi Cui,Long Liu

Physical biology 16:035002 PubMed30769341

2019

Facile single-molecule pull-down assay for analysis of endogenous proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin Croop,Kyu Young Han

Journal of cellular and molecular medicine 22:3330-3339 PubMed29570934

2018

Non-erythropoietic erythropoietin-derived peptide protects mice from systemic lupus erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Huang,Juntao Jiang,Bangwei Luo,Wen Zhu,Yuqi Liu,Zhishang Wang,Zhiren Zhang

Lab on a chip 16:1340-5 PubMed26980325

2016

Digital diffraction detection of protein markers for avian influenza.

Applications

Unspecified application

Species

Unspecified reactive species

Hyungsoon Im,Yong Il Park,Divya Pathania,Cesar M Castro,Ralph Weissleder,Hakho Lee

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat Anti-Mouse IgG H&L (Biotin) preadsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (8)

Complementary Products

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise