Goat Anti-Mouse IgG H&L (Cy3 ®) is a preadsorbed secondary antibody that is suitable for ICC/IF.
- Preadsorbed to prevent cross-reactivity and reduce background staining
- Cited in over 140 publications
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Publications
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- Nature communications 15:4382024Disparate macrophage responses are linked to infection outcome of Hantan virus in humans or rodents.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongwei Ma,Yongheng Yang,Tiejian Nie,Rong Yan,Yue Si,Jing Wei,Mengyun Li,He Liu,Wei Ye,Hui Zhang,Linfeng Cheng,Liang Zhang,Xin Lv,Limin Luo,Zhikai Xu,Xijing Zhang,Yingfeng Lei,Fanglin ZhangPubMed 38200007
- International journal of medical sciences 21:137-1502024Enhanced Bone Regeneration through Regulation of Mechanoresponsive FAK-ERK1/2 Signaling by ZINC40099027 during Distraction Osteogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShanyu Li,Hongxiao Wu,Feng Wang,Lingchi Kong,Yifan Yu,Rongtai Zuo,Haoyu Zhao,Jia Xu,Qinglin KangPubMed 38164350
- Life science alliance 7:2023DCAF14 regulates CDT2 to promote SET8-dependent replication fork protection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNeysha Tirado-Class,Caitlin Hathaway,Anthony Nelligan,Thuan Nguyen,Huzefa DungrawalaPubMed 37940188
- Nature communications 14:61732023The USP46 complex deubiquitylates LRP6 to promote Wnt/β-catenin signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVictoria H Ng,Zachary Spencer,Leif R Neitzel,Anmada Nayak,Matthew A Loberg,Chen Shen,Sara N Kassel,Heather K Kroh,Zhenyi An,Christin C Anthony,Jamal M Bryant,Amanda Lawson,Lily Goldsmith,Hassina Benchabane,Amanda G Hansen,Jingjing Li,Starina D'Souza,Andres M Lebensohn,Rajat Rohatgi,William A Weiss,Vivian L Weiss,Charles Williams,Charles C Hong,David J Robbins,Yashi Ahmed,Ethan LeePubMed 37798301
- Microbiome 11:1542023High-fat diet-disturbed gut microbiota-colonocyte interactions contribute to dysregulating peripheral tryptophan-kynurenine metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPenghao Sun,Mengli Wang,Yong-Xin Liu,Luqi Li,Xuejun Chai,Wei Zheng,Shulin Chen,Xiaoyan Zhu,Shanting ZhaoPubMed 37468922
- Foods (Basel, Switzerland) 12:2023"Biqi" Bayberry Extract Promotes Skeletal Muscle Fiber Type Remodeling by Increasing Fast Myofiber Formation via the Akt/FoxO1 Pathway in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinjie Li,Yi Li,Xiangying Suo,Jiangtao Li,Da Huang,Guangning KouPubMed 37444209
- Nature communications 14:29622023An mRNA-based T-cell-inducing antigen strengthens COVID-19 vaccine against SARS-CoV-2 variants.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWanbo Tai,Shengyong Feng,Benjie Chai,Shuaiyao Lu,Guangyu Zhao,Dong Chen,Wenhai Yu,Liting Ren,Huicheng Shi,Jing Lu,Zhuming Cai,Mujia Pang,Xu Tan,Penghua Wang,Jinzhong Lin,Qiangming Sun,Xiaozhong Peng,Gong ChengPubMed 37221158
- Molecular medicine (Cambridge, Mass.) 29:552023FUT1-mediated terminal fucosylation acts as a new target to attenuate renal fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJialiang Luo,Kaifeng Mao,Zhengyumeng Zhu,Junli Ye,Lei Li,Di Wang,Jia Zhou,Fenwang Lin,Juan Li,Junsheng YePubMed 37085770
- Nature communications 14:19252023Replication-associated formation and repair of human topoisomerase IIIα cleavage complexes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiton Kumar Saha,Sourav Saha,Xi Yang,Shar-Yin Naomi Huang,Yilun Sun,Ukhyun Jo,Yves PommierPubMed 37024461
- International journal of molecular sciences 24:2023Mast Cell Tryptase and Carboxypeptidase A3 in the Formation of Ovarian Endometrioid Cysts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDmitri Atiakshin,Olga Patsap,Andrey Kostin,Lyudmila Mikhalyova,Igor Buchwalow,Markus TiemannPubMed 37047472
Key facts
- Host species
- Goat
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Rat, Sheep, Rabbit, Goat, Horse, Chicken, Cow, Human, Pig
- Conjugation
- Cy3®
- Applications
- ICC/IF
- Clonality
- Polyclonal
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC/IF | Reactivity Reacts | Dilution info 1/50.00000 - 1/500.00000 | Notes - |
Associated Products
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Target data
Additional Targets
Alternative names
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form
Recommended products
Goat Anti-Mouse IgG H&L (Cy3 ®) is a preadsorbed secondary antibody that is suitable for ICC/IF.
- Preadsorbed to prevent cross-reactivity and reduce background staining
- Cited in over 140 publications
Key facts
- Description
- Goat Anti-Mouse IgG H&L (Cy3 ®) preadsorbed
- Host species
- Goat
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Rat, Sheep, Rabbit, Goat, Horse, Chicken, Cow, Human, Pig
- Conjugation
- Cy3®
- Applications
- ICC/IF
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% PBS, 0.2% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy3®.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
This antibody reacts with the heavy and light chains of Mouse IgG
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2 product images
Image courtesy of Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, CanadaImmunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Cy3 ®) preadsorbed (ab97035)
ab97035 (1/200) detecting Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] ab14955 in assynchronous HeLa cells (red). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] ab14955, a mouse monoclonal anti-Histone H3 (phospho S10) antibody, was used at a 1/2000 dilution.
Wu JL et al. Cloning and Functional Analysis of Histones H3 and H4 in Nuclear Shaping during Spermatogenesis of the Chinese Mitten Crab, Eriocheir sinensis. PLoS One 10:e0126623 (2015).Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Cy3 ®) preadsorbed (ab97035)
(a–c) Immunofluorescent localization of histone H4 in early spermatids. (d) Ultrastructural localization of histone H4 in early spermatids. (e) Ultrastructural localization of histone H3 in intermediate spermatids. The secondary antibody used was Goat Anti-Mouse IgG H&L (Cy3 ® )preadsorbed (ab97035) at 1/200 dilution.
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