AB97035

Goat Anti-Mouse IgG H&L (Cy3 ®) preadsorbed

Name: Goat Anti-Mouse IgG (Cy3®) preadsorbed secondary antibody (ab97035) | Abcam
Brand: Abcam Limited
SKU: AB97035
Price: 235 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(200 Publications)

Goat Anti-Mouse IgG H&L (Cy3 ®) is a preadsorbed secondary antibody that is suitable for ICC/IF.

- Preadsorbed to prevent cross-reactivity and reduce background staining
- Cited in over 140 publications

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

2 Images

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Cy3 ®) preadsorbed (AB97035)

ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Cy3 ®) preadsorbed (AB97035)

(a–c) Immunofluorescent localization of histone H4 in early spermatids. (d) Ultrastructural localization of histone H4 in early spermatids. (e) Ultrastructural localization of histone H3 in intermediate spermatids. The secondary antibody used was Goat Anti-Mouse IgG H&L (Cy3 ^® )preadsorbed (ab97035) at 1/200 dilution.

Wu JL et al. Cloning and Functional Analysis of Histones H3 and H4 in Nuclear Shaping during Spermatogenesis of the Chinese Mitten Crab, Eriocheir sinensis. PLoS One 10:e0126623 (2015).

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Cy3 ®) preadsorbed (AB97035)

ab97035 (1/200) detecting ab14955 in assynchronous HeLa cells (red). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). ab14955, a mouse monoclonal anti-Histone H3 (phospho S10) antibody, was used at a 1/2000 dilution.

Image courtesy of Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Sheep, Rabbit, Goat, Horse, Chicken, Cow, Human, Pig

Pre-adsorbed

Yes

Conjugation

Cy3®

Excitation/Emission

Ex: 552nm, Em: 565nm

Applications

ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"<p></p>" } } }

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Product details

This antibody reacts with the heavy and light chains of Mouse IgG

Fluorochrome chart- a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy3®.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (200)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 31:e70618 PubMed40994415

2025

Congenital Hypothyroidism Dysregulates TRPC6 to Mediate Abnormal Dendritic Spine Growth of Hippocampal Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Tianci Li,Feifei Shen,Lingling Li,Peng Chen,Zhiwen Zhu,Yuqin Zheng,Haiying Li,Guihai Suo,Yongjun Wang,Jinlong Shi,Youjia Wu

Medical sciences (Basel, Switzerland) 13: PubMed40981193

2025

Mast Cell Association with the Microenvironment of a Phosphaturic Mesenchymal Tumour Secreting Fibroblast Growth Factor 23.

Applications

Unspecified application

Species

Unspecified reactive species

Andrey Kostin,Alexei Lyundup,Alexander Alekhnovich,Aleksandra Prikhodko,Olga Patsap,Sofia Gronskaia,Zhanna Belaya,Olga Lesnyak,Galina Melnichenko,Natalia Mokrysheva,Igor Buchwalow,Markus Tiemann,Dmitrii Atiakshin

Cell discovery 11:74 PubMed40921762

2025

Intrauterine hyperglycemia impairs mouse primordial germ cell development and fertility by sex-specific epigenetic reprogramming interference.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangshan Cong,Qing Li,Yangyang Li,Minghao Li,Yan Shi,Peiran Hu,Xidi Yin,Qianyun Zhang,Jianzhong Sheng,Jinsong Li,Guolian Ding,Yu Zhang,Hefeng Huang

Molecular therapy. Oncology 33:201028 PubMed40896366

2025

is a synthetic lethal interactor of in colonic epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tooba Razi,Ally C Farrell,Rubi Campos Gudiño,Nicole M Neudorf,Zelda Lichtensztejn,Kirk J McManus

The Journal of cell biology 224: PubMed40627464

2025

Myosin 15 participates in assembly and remodeling of the presynapse.

Applications

Unspecified application

Species

Unspecified reactive species

Astrid G Petzoldt,Marc J F Escher,Oriane Turrel,Niclas Gimber,Ina M Schedina,Sophie Walter,Torsten W B Götz,Marta Maglione,David Toppe,Tanja Matkovic-Rachid,Alexander Neumann,Janine Lützkendorf,Jan Schmoranzer,Martin Lehmann,Jörg Großhans,Stephan J Sigrist

Cell death & disease 16:470 PubMed40593489

2025

PKIB facilitates bladder cancer proliferation and metastasis through mediation of HSP27 phosphorylation by PKA.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolong Liu,Xiaoyu Yin,Feng Yuan,Shiqing Li,Fei Xiao,Feng Zhou,Xudong Pan,Yatfaat Ho,Shuo Dong,Duan Xu,Yunqing Ma,Zhengding Cao,Zhe Lei,Yi Sun

International journal of molecular sciences 26: PubMed40565000

2025

SARS-CoV-2 ORF7a Protein Impedes Type I Interferon-Activated JAK/STAT Signaling by Interacting with HNRNPA2B1.

Applications

Unspecified application

Species

Unspecified reactive species

Yujie Wen,Chaochao Li,Tian Tang,Chao Luo,Shan Lu,Na Lyu,Yongxi Li,Rong Wang

Mediators of inflammation 2025:7248780 PubMed40548298

2025

CCL2 Inhibitor Bindarit Improve Postoperative Cognitive Function by Attenuating Pericyte Loss-Related Blood-Brain Barrier Disruption and Neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Yuan,Bo Lu,Daofan Sun,Junping Chen,Xiangming Fang

Journal of translational medicine 23:647 PubMed40500704

2025

The SOX18-OTUB1-YAP1 axis: a new endometriosis target.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Feng,Jiamei Yue,Si Fan,Jiayan Wu

Nature communications 16:5248 PubMed40473626

2025

The TRIP12 E3 ligase induces SWI/SNF component BRG1-β-catenin interaction to promote Wnt signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Kassel,Kai Yuan,Nawat Bunnag,Leif R Neitzel,Wenhui Lu,Anna Schwarzkopf,Benjamin Maines,Matthew A Loberg,George Xu,Amber Adams,Andrew D McCray,Alex Cho,Mary Rockouski,Gemma Orton,Lily Goldsmith,Md Mubtaseem Ahnaf Aronno,Zachary T Spencer,Omar M Khan,Fei Ye,Charles Williams,Andres M Lebensohn,Rajat Rohatgi,Xiaofeng Wang,Vivian L Weiss,Charles C Hong,Arminja N Kettenbach,David J Robbins,Yashi Ahmed,Ethan Lee

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat Anti-Mouse IgG H&L (Cy3 ®) preadsorbed

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Cy3 ®) preadsorbed (AB97035)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Cy3 ®) preadsorbed (AB97035)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Additional targets

Publications (200)

Product promise