Goat Anti-Mouse IgG H&L (Cy5 ®) preadsorbed

2 product images

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  Antanaviciute et al PLoS One. 2014 Jun 19;9(6):e99196. doi: 10.1371/journal.pone.0099196. eCollection 2014. Fig 4. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

  Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Cy5 ®) preadsorbed (ab6563)

  Formation of TT3s between LSCC cells.
  (Panel G) TT4s form between the intersecting rear or secondary lamellipodia establishing functional Cx43 GJs (the arrow in the inset) as confirmed by patch-clamp measurements.
  

  Cells were grown in 24-well plates with glass coverslips on the bottom, fixed with 4% paraformaldehyde for 15 min, and permeabilized with 0.2% Triton X-100/PBS for 3 min. Coverslips were incubated for 1 h with the following primary antibodies: mouse anti-α-tubulin, rabbit anti-Cx43, mouse anti-Cx43, rabbit anti-Cx26, rabbit anti-Cx30, then rinsed with 1% BSA/PBS and incubated with secondary goat anti-mouse IgG H&L (Cy5) (ab6563, Abcam Cambridge, UK) or with donkey anti-rabbit IgG (FITC) for 30 min. The F-actin network was visualized using Alexa Fluor 594 phalloidin; coverslips were incubated with the dye for 30 min at 37°C. Coverglasses were attached using Vectashield Mounting Medium with DAPI and sealed with clear nail polish. MitoTracker Green was used to stain mitochondria in live cells.

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  This image is courtesy of a customer review submitted by Dr laurent barbe

  Immunocytochemistry - Goat Anti-Mouse IgG H&L (Cy5 ®) preadsorbed (ab6563)

  Anti-P4HB antibody [RL90] ab2792 (PDI antibody (RL90) - ER marker) staining human U2-OS (bone osteosarcoma) cells by ICC/IF. ab6563 (undiluted) was used as the secondary.