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AB97037

Goat Anti-Mouse IgG H&L (Cy5 ®) preadsorbed

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(13 Publications)

Suitable for ICC/IF, Flow Cyt, IHC-P. Preadsorbed to minimise non-specific binding and high background staining. Cited in 13 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Sheep, Rabbit, Goat, Horse, Chicken, Cow, Human

Pre-adsorbed

Yes

Conjugation

Cy5®

Excitation/Emission

Ex: 650nm, Em: 667nm

Applications

Flow Cyt, ICC/IF, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/1000", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy5 ®.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Product protocols

Target data

See full target information Ighg1

Additional targets

Ighg1

Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Methods in molecular biology (Clifton, N.J.) 2668:257-276 PubMed37140802

2023

Magnetic Separation of Cell-Secreted Vesicles with Tailored Magnetic Particles and Downstream Applications.

Applications

Unspecified application

Species

Unspecified reactive species

Mireia Bernuz,Arnau Pallarès-Rusiñol,Rosanna Rossi,Carolina Fernández-Senac,Mercè Martí,María Isabel Pividori

Analytical chemistry 95:2487-2495 PubMed36683335

2023

Electrochemical Genosensing of Overexpressed GAPDH Transcripts in Breast Cancer Exosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Arnau Pallares-Rusiñol,Silio Lima Moura,Mercè Martí,Maria Isabel Pividori

Dental materials : official publication of the Academy of Dental Materials 39:204-216 PubMed36642687

2023

Immunomodulatory IL-23 receptor antagonist peptide nanocoatings for implant soft tissue healing.

Applications

Unspecified application

Species

Unspecified reactive species

John A Pizarek,Nicholas G Fischer,Conrado Aparicio

Cell 185:3931-3949.e26 PubMed36240740

2022

GPC3-Unc5 receptor complex structure and role in cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Onno Akkermans,Céline Delloye-Bourgeois,Claudia Peregrina,Maria Carrasquero-Ordaz,Maria Kokolaki,Miguel Berbeira-Santana,Matthieu Chavent,Florie Reynaud,Ritu Raj,Jon Agirre,Metin Aksu,Eleanor S White,Edward Lowe,Dounia Ben Amar,Sofia Zaballa,Jiandong Huo,Irene Pakos,Patrick T N McCubbin,Davide Comoletti,Raymond J Owens,Carol V Robinson,Valérie Castellani,Daniel Del Toro,Elena Seiradake

Frontiers in neurology 13:877342 PubMed35493807

2022

Purification of Fibroblasts From the Spiral Ganglion.

Applications

Unspecified application

Species

Unspecified reactive species

Annett Anacker,Karl-Heinz Esser,Thomas Lenarz,Gerrit Paasche

Stem cell research & therapy 12:604 PubMed34922628

2021

Culture and characterization of various porcine integumentary-connective tissue-derived mesenchymal stromal cells to facilitate tissue adhesion to percutaneous metal implants.

Applications

Unspecified application

Species

Unspecified reactive species

Devaveena Dey,Nicholas G Fischer,Andrea H Dragon,Elsa Ronzier,Isha Mutreja,David T Danielson,Cole J Homer,Jonathan A Forsberg,Joan E Bechtold,Conrado Aparicio,Thomas A Davis

Biosensors & bioelectronics 198:113826 PubMed34891059

2021

The activity of alkaline phosphatase in breast cancer exosomes simplifies the biosensing design.

Applications

Unspecified application

Species

Unspecified reactive species

Silio Lima Moura,Arnau Pallarès-Rusiñol,Luciano Sappia,Mercè Martí,María Isabel Pividori

Coatings (Basel, Switzerland) 10: PubMed32855816

2020

Surface Immobilization Chemistry of a Laminin-Derived Peptide Affects Keratinocyte Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas G Fischer,Jiahe He,Conrado Aparicio

Sensors (Basel, Switzerland) 20: PubMed32054015

2020

Matrix Effect in the Isolation of Breast Cancer-Derived Nanovesicles by Immunomagnetic Separation and Electrochemical Immunosensing-A Comparative Study.

Applications

Unspecified application

Species

Unspecified reactive species

Silio Lima Moura,Mercè Martì,María Isabel Pividori

Journal of cell science 130:779-790 PubMed28062850

2017

Cellular strain avoidance is mediated by a functional actin cap - observations in an -deficient cell model.

Applications

Unspecified application

Species

Unspecified reactive species

Chiara Tamiello,Maurice Halder,Miriam A F Kamps,Frank P T Baaijens,Jos L V Broers,Carlijn V C Bouten
View all publications

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