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AB96871

Goat Anti-Mouse IgG H&L (DyLight® 488)

5

(3 Reviews)

|

(22 Publications)

Suitable for IHC-P, Flow Cyt, ICC/IF. Cited in 22 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form

1 Images
Flow Cytometry - Goat Anti-Mouse IgG H&L (DyLight® 488) (AB96871)
  • Flow Cyt

Unknown

Flow Cytometry - Goat Anti-Mouse IgG H&L (DyLight® 488) (AB96871)

Overlay histogram showing Jurkat cells stained with ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8090, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody Goat anti-mouse IgG H&L (DyLight® 488) (ab96871) was used at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.01μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

DyLight® 488

Excitation/Emission

Ex: 493nm, Em: 518nm

Applications

IHC-P, Flow Cyt, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/2000", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

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Target data

See full target information Ighg1

Additional targets

Ighg1
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Publications (22)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 12:22641 PubMed36587177

2022

Live probiotic bacteria administered in a pathomimetic Leaky Gut Chip ameliorate impaired epithelial barrier and mucosal inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Soyoun Min,Nam Than,Yong Cheol Shin,Grace Hu,Woojung Shin,Yoko M Ambrosini,Hyun Jung Kim

iScience 25:105521 PubMed36425760

2022

Single-cell transcriptomic mapping of intestinal epithelium that undergoes 3D morphogenesis and mechanodynamic stimulation in a gut-on-a-chip.

Applications

Unspecified application

Species

Unspecified reactive species

Woojung Shin,Zhe Su,S Stephen Yi,Hyun Jung Kim

Nature protocols 17:910-939 PubMed35110737

2022

3D in vitro morphogenesis of human intestinal epithelium in a gut-on-a-chip or a hybrid chip with a cell culture insert.

Applications

Unspecified application

Species

Unspecified reactive species

Woojung Shin,Hyun Jung Kim

Journal of orthopaedic surgery and research 17:53 PubMed35093114

2022

Thioacetamide promotes osteoclast transformation of bone marrow macrophages by influencing PI3K/AKT pathways.

Applications

Unspecified application

Species

Unspecified reactive species

XiaoLi Jin,Yang Li,Yayang Yang,Hao Shen,Jin Chen,Bin Xu,Jian Xu

Autophagy 18:1841-1863 PubMed34872436

2021

Inhibition of PLA2G4E/cPLA2 promotes survival of random skin flaps by alleviating Lysosomal membrane permeabilization-Induced necroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Junsheng Lou,Xiangyang Wang,Haojie Zhang,Gaoxiang Yu,Jian Ding,Xuwei Zhu,Yao Li,Yaosen Wu,Hui Xu,Huazi Xu,Weiyang Gao,Jian Xiao,Kailiang Zhou

Experimental biology and medicine (Maywood, N.J.) 247:416-425 PubMed34727745

2021

Intranasal administration of mitochondria improves spatial memory in olfactory bulbectomized mice.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia V Bobkova,Daria Y Zhdanova,Natalia V Belosludtseva,Nikita V Penkov,Galina D Mironova

Analytical methods : advancing methods and applications 13:1191-1203 PubMed33605950

2021

Nonspecific nuclear uptake of anti-MUC1 aptamers by dead cells: the role of cell viability monitoring in aptamer targeting of membrane-bound protein cancer biomarkers.

Applications

Unspecified application

Species

Unspecified reactive species

Shane Patrick Flanagan,Ronen Fogel,Adrienne Lesley Edkins,Lance St John Ho,Janice Limson

Cell metabolism 33:283-299.e9 PubMed33400911

2021

Platelets Facilitate the Wound-Healing Capability of Mesenchymal Stem Cells by Mitochondrial Transfer and Metabolic Reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Jennyfer Levoux,Alexandre Prola,Peggy Lafuste,Marianne Gervais,Nathalie Chevallier,Zeynab Koumaiha,Kaouthar Kefi,Laura Braud,Alain Schmitt,Azzedine Yacia,Aurélie Schirmann,Barbara Hersant,Mounia Sid-Ahmed,Sabrina Ben Larbi,Katerina Komrskova,Jakub Rohlena,Frederic Relaix,Jiri Neuzil,Anne-Marie Rodriguez

Brain, behavior, and immunity 93:288-298 PubMed33401017

2021

Elastase inhibitor agaphelin protects from acute ischemic stroke in mice by reducing thrombosis, blood-brain barrier damage, and inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Jonas Leinweber,Daniella M Mizurini,Ivo M B Francischetti,Michael Fleischer,Dirk M Hermann,Christoph Kleinschnitz,Friederike Langhauser

Lab on a chip 20:3423-3434 PubMed32785399

2020

Development of a cardiac-and-piezoelectric hybrid system for application in drug screening.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-Han Huang,Chiou-Fong Yang,Yu-Hsiang Hsu
View all publications
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