Suitable for IHC-P, Flow Cyt, ICC/IF. Cited in 22 publications.
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- Scientific reports 12:226412022Live probiotic bacteria administered in a pathomimetic Leaky Gut Chip ameliorate impaired epithelial barrier and mucosal inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSoyoun Min,Nam Than,Yong Cheol Shin,Grace Hu,Woojung Shin,Yoko M Ambrosini,Hyun Jung KimPubMed 36587177
- iScience 25:1055212022Single-cell transcriptomic mapping of intestinal epithelium that undergoes 3D morphogenesis and mechanodynamic stimulation in a gut-on-a-chip.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWoojung Shin,Zhe Su,S Stephen Yi,Hyun Jung KimPubMed 36425760
- Nature protocols 17:910-93920223D in vitro morphogenesis of human intestinal epithelium in a gut-on-a-chip or a hybrid chip with a cell culture insert.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWoojung Shin,Hyun Jung KimPubMed 35110737
- Journal of orthopaedic surgery and research 17:532022Thioacetamide promotes osteoclast transformation of bone marrow macrophages by influencing PI3K/AKT pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoLi Jin,Yang Li,Yayang Yang,Hao Shen,Jin Chen,Bin Xu,Jian XuPubMed 35093114
- Autophagy 18:1841-18632021Inhibition of PLA2G4E/cPLA2 promotes survival of random skin flaps by alleviating Lysosomal membrane permeabilization-Induced necroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunsheng Lou,Xiangyang Wang,Haojie Zhang,Gaoxiang Yu,Jian Ding,Xuwei Zhu,Yao Li,Yaosen Wu,Hui Xu,Huazi Xu,Weiyang Gao,Jian Xiao,Kailiang ZhouPubMed 34872436
- Experimental biology and medicine (Maywood, N.J.) 247:416-4252021Intranasal administration of mitochondria improves spatial memory in olfactory bulbectomized mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatalia V Bobkova,Daria Y Zhdanova,Natalia V Belosludtseva,Nikita V Penkov,Galina D MironovaPubMed 34727745
- Analytical methods : advancing methods and applications 13:1191-12032021Nonspecific nuclear uptake of anti-MUC1 aptamers by dead cells: the role of cell viability monitoring in aptamer targeting of membrane-bound protein cancer biomarkers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShane Patrick Flanagan,Ronen Fogel,Adrienne Lesley Edkins,Lance St John Ho,Janice LimsonPubMed 33605950
- Cell metabolism 33:283-299.e92021Platelets Facilitate the Wound-Healing Capability of Mesenchymal Stem Cells by Mitochondrial Transfer and Metabolic Reprogramming.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJennyfer Levoux,Alexandre Prola,Peggy Lafuste,Marianne Gervais,Nathalie Chevallier,Zeynab Koumaiha,Kaouthar Kefi,Laura Braud,Alain Schmitt,Azzedine Yacia,Aurélie Schirmann,Barbara Hersant,Mounia Sid-Ahmed,Sabrina Ben Larbi,Katerina Komrskova,Jakub Rohlena,Frederic Relaix,Jiri Neuzil,Anne-Marie RodriguezPubMed 33400911
- Brain, behavior, and immunity 93:288-2982021Elastase inhibitor agaphelin protects from acute ischemic stroke in mice by reducing thrombosis, blood-brain barrier damage, and inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJonas Leinweber,Daniella M Mizurini,Ivo M B Francischetti,Michael Fleischer,Dirk M Hermann,Christoph Kleinschnitz,Friederike LanghauserPubMed 33401017
- Lab on a chip 20:3423-34342020Development of a cardiac-and-piezoelectric hybrid system for application in drug screening.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun-Han Huang et. al.PubMed 32785399
Key facts
- Host species
- Goat
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- DyLight® 488
- Applications
- IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC-P | Reactivity Reacts | Dilution info 1/50.00000 - 1/500.00000 | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/1000.00000 - 1/2000.00000 | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/50.00000 - 1/500.00000 | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Additional Targets
Alternative names
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form
Recommended products
Suitable for IHC-P, Flow Cyt, ICC/IF. Cited in 22 publications.
Key facts
- Description
- Goat Anti-Mouse IgG H&L (DyLight® 488)
- Host species
- Goat
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- DyLight® 488
- Applications
- IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Product promise
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1 product image
Flow Cytometry - Goat Anti-Mouse IgG H&L (DyLight® 488) (ab96871)
Overlay histogram showing Jurkat cells stained with Anti-CD3 antibody [MEM-57] ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-CD3 antibody [MEM-57] ab8090, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody Goat anti-mouse IgG H&L (DyLight® 488) (ab96871) was used at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control ab91361, 0.01μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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