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Suitable for IHC-P, Flow Cyt, ICC/IF, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 74 publications.

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Images

Flow Cytometry - Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (AB96879), expandable thumbnail
  • Immunocytochemistry - Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (AB96879), expandable thumbnail
  • Flow Cytometry (Intracellular) - Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (AB96879), expandable thumbnail
  • Flow Cytometry - Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (AB96879), expandable thumbnail
  • Flow Cytometry - Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (AB96879), expandable thumbnail

Publications

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Rabbit, Goat, Horse, Chicken, Cow, Human, Pig

Conjugation

DyLight® 488

Applications

IHC-P, Flow Cyt, ICC/IF, WB

Clonality

Polyclonal

Abcam Recommends

Reactivity data

Application

IHC-P

Reactivity

Reacts

Dilution info

1/50.00000 - 1/500.00000

Notes

-

Application

Flow Cyt

Reactivity

Reacts

Dilution info

1/1000.00000 - 1/2000.00000

Notes

-

Application

ICC/IF

Reactivity

Reacts

Dilution info

1/50.00000 - 1/500.00000

Notes

-

Application

WB

Reactivity

Reacts

Dilution info

1/1000.00000 - 1/20000.00000

Notes

5% non-fat dry milk in PBST or TBST is recommended for blocking and incubation of antibodies. BSA is not recommended.

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Suitable for IHC-P, Flow Cyt, ICC/IF, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 74 publications.

Key facts

Description

Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed

Host species

Goat

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Rabbit, Goat, Horse, Chicken, Cow, Human, Pig

Conjugation

DyLight® 488

Applications

IHC-P, Flow Cyt, ICC/IF, WB

Clonality

Polyclonal

Pre-adsorbed

Yes

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.

Isotype

IgG

Concentration
Loading...

Properties

Form

Liquid

Storage buffer

pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Flow Cytometry - Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879), expandable thumbnail

    Flow Cytometry - Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)

    Overlay histogram showing Jurkat cells stained with Anti-CD3 antibody [MEM-57] ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-CD3 antibody [MEM-57] ab8090, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody Goat anti-mouse IgG H&L (DyLight® 488, pre-adsorbed) (ab96879) was used at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control ab91361, 0.01μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Immunocytochemistry - Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879), expandable thumbnail

    Immunocytochemistry - Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)

    ICC/IF image of ab40084 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40084, 5μg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

  • Flow Cytometry (Intracellular) - Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879), expandable thumbnail

    Flow Cytometry (Intracellular) - Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)

    Overlay histogram showing HeLa cells stained with Anti-Histone H2B antibody [mAbcam 64165] - ChIP Grade ab64165 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-Histone H2B antibody [mAbcam 64165] - ChIP Grade ab64165, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (Mouse IgG2b [PLPV219] - Isotype Control ab91366, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Flow Cytometry - Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879), expandable thumbnail

    Flow Cytometry - Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)

    Overlay histogram showing HepG2 (Human liver hepatocellular carcinoma cell line) cells stained with ab2861 (red line).
    The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2861, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) ab96879 at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control ab91361, 1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Flow Cytometry - Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879), expandable thumbnail

    Flow Cytometry - Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)

    Flow cytometry overlay histogram of 4% formaldehyde fixed Jurkat cells permeabilized with 90% methanol labeling PCNA with Anti-PCNA antibody [PC10] ab265585 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details, DyLight® 488-conjugated goat anti-mouse IgG (ab96879).

  • Flow Cytometry (Intracellular) - Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879), expandable thumbnail

    Flow Cytometry (Intracellular) - Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)

  • Flow Cytometry - Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879), expandable thumbnail
    This image is courtesy of an anonymous customer review.

    Flow Cytometry - Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)

    ab85137 staining S100 in a human melanoma cell line by Flow Cytometry. The cells were harvested using EDTA and washed in PBS. The sample was incubated with the primary antibody (1/100 in PBS) for 15 minutes at room temperature. A DyLight® 488-conjugated goat anti-mouse IgG H&L (ab96879) (1/100) was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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