AB96880

Goat Anti-Mouse IgG H&L (DyLight® 550) preadsorbed

Name: Goat Anti Mouse (IgG) secondary antibody preadsorbed DyLight® 550 (ab96880)
Brand: Abcam Limited
SKU: AB96880

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(13 Publications)

Suitable for ICC/IF, Flow Cyt, IHC-P. Preadsorbed to minimise non-specific binding and high background staining. Cited in 13 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Rabbit, Goat, Horse, Chicken, Cow, Human, Pig

Pre-adsorbed

Yes

Conjugation

DyLight® 550

Excitation/Emission

Ex: 562nm, Em: 576nm

Applications

IHC-P, Flow Cyt, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments." } } }

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Product details

DyLight® 550 replaces DyLight® 549.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadp8093 PubMed40215293

2025

Loss of Ten1 in mice induces telomere shortening and models human dyskeratosis congenita.

Applications

Unspecified application

Species

Unspecified reactive species

Adrián Sanz-Moreno,Lore Becker,Kan Xie,Patricia da Silva-Buttkus,Nathalia R V Dragano,Antonio Aguilar-Pimentel,Oana V Amarie,Julia Calzada-Wack,Markus Kraiger,Stefanie Leuchtenberger,Claudia Seisenberger,Susan Marschall,Birgit Rathkolb,Enzo Scifo,Ting Liu,Anoja Thanabalasingam,Raul Sanchez-Vazquez,Paula Martinez,Maria A Blasco,Sharon A Savage,Helmut Fuchs,Dan Ehninger,Valérie Gailus-Durner,Martin Hrabê de Angelis

Cell structure and function 49:21-29 PubMed38797697

2024

A high-resolution phase-contrast microscopy system for label-free imaging in living cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kentaro Shimasaki,Yuko Okemoto-Nakamura,Kyoko Saito,Masayoshi Fukasawa,Kaoru Katoh,Kentaro Hanada

International journal of molecular sciences 25: PubMed38732109

2024

Melatonin Enhances Neural Differentiation of Adipose-Derived Mesenchymal Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ivana Roberta Romano,Floriana D'Angeli,Elisa Gili,Mary Fruciano,Giuseppe Angelo Giovanni Lombardo,Giuliana Mannino,Nunzio Vicario,Cristina Russo,Rosalba Parenti,Carlo Vancheri,Rosario Giuffrida,Rosalia Pellitteri,Debora Lo Furno

World journal of stem cells 13:1783-1796 PubMed34909123

2021

ARPE-19 conditioned medium promotes neural differentiation of adipose-derived mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Giuliana Mannino,Martina Cristaldi,Giovanni Giurdanella,Rosario Emanuele Perrotta,Debora Lo Furno,Rosario Giuffrida,Dario Rusciano

eLife 10: PubMed34269678

2021

Homo-oligomerization of the human adenosine A receptor is driven by the intrinsically disordered C-terminus.

Applications

Unspecified application

Species

Unspecified reactive species

Khanh Dinh Quoc Nguyen,Michael Vigers,Eric Sefah,Susanna Seppälä,Jennifer Paige Hoover,Nicole Star Schonenbach,Blake Mertz,Michelle Ann O'Malley,Songi Han

Journal of bioenergetics and biomembranes 50:339-354 PubMed29998379

2018

The complexity of mitochondrial outer membrane permeability and VDAC regulation by associated proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Aleksandr Klepinin,Lyudmila Ounpuu,Kati Mado,Laura Truu,Vladimir Chekulayev,Marju Puurand,Igor Shevchuk,Kersti Tepp,Anu Planken,Tuuli Kaambre

FEBS letters 590:3295-306 PubMed27543907

2016

Adenosine A2a receptors form distinct oligomers in protein detergent complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole S Schonenbach,Monica D Rieth,Songi Han,Michelle A O'Malley

Antioxidants & redox signaling 25:41-58 PubMed27059697

2016

Redox Indicator Mice Stably Expressing Genetically Encoded Neuronal roGFP: Versatile Tools to Decipher Subcellular Redox Dynamics in Neuropathophysiology.

Applications

ICC/IF

Species

Unspecified reactive species

Kerstin C Wagener,Benedikt Kolbrink,Katharina Dietrich,Kathrin M Kizina,Lukas S Terwitte,Belinda Kempkes,Guobin Bao,Michael Müller

Cancer biology & therapy 17:526-42 PubMed26828826

2016

Pluripotency markers are differentially induced by MEK inhibition in thyroid and melanoma BRAFV600E cell lines.

Applications

Species

Unspecified reactive species

Emma R Dorris,Gordon Blackshields,Gary Sommerville,Mohsen Alhashemi,Andrew Dias,Victoria McEneaney,Paul Smyth,John J O'Leary,Orla Sheils

The international journal of biochemistry & cell b 55:171-86 PubMed25218857

2014

An in situ study of bioenergetic properties of human colorectal cancer: the regulation of mitochondrial respiration and distribution of flux control among the components of ATP synthasome.

Applications

IHC-P

Species

Unspecified reactive species

Andrus Kaldma,Aleksandr Klepinin,Vladimir Chekulayev,Kati Mado,Igor Shevchuk,Natalja Timohhina,Kersti Tepp,Manana Kandashvili,Minna Varikmaa,Andre Koit,Margus Planken,Karoliina Heck,Laura Truu,Anu Planken,Vahur Valvere,Egle Rebane,Tuuli Kaambre

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat Anti-Mouse IgG H&L (DyLight® 550) preadsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Additional targets

Publications (13)

Product promise