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Suitable for IHC-P, Flow Cyt, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 26 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed (AB96881), expandable thumbnail

Publications

Key facts

Host species
Goat
Target species
Mouse
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Rat, Rabbit, Goat, Horse, Chicken, Cow, Human, Pig
Conjugation
DyLight® 594
Applications
IHC-P, Flow Cyt, ICC/IF
Clonality
Polyclonal

Reactivity data

Application
IHC-P
Reactivity
Reacts
Dilution info
1/50.00000 - 1/500.00000
Notes

-

Application
Flow Cyt
Reactivity
Reacts
Dilution info
1/50.00000 - 1/200.00000
Notes

-

Application
ICC/IF
Reactivity
Reacts
Dilution info
1/50.00000 - 1/500.00000
Notes

-

Associated Products

Select an associated product type

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Target data

Additional Targets

Alternative names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form

Recommended products

Suitable for IHC-P, Flow Cyt, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 26 publications.

Key facts

Description
Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed
Host species
Goat
Target species
Mouse
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Rat, Rabbit, Goat, Horse, Chicken, Cow, Human, Pig
Conjugation
DyLight® 594
Applications
IHC-P, Flow Cyt, ICC/IF
Clonality
Polyclonal
Pre-adsorbed
Yes
Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.

Isotype
IgG
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA

Purification technique
Affinity purification Immunogen
Purification notes

Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Product promise

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Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed (ab96881), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed (ab96881)

    ab96881 was used at dilution 1/100 for IHC analysis of FFPE human kidney. Primary Antibody: mouse anti-p53 (clone DO-1) used at a dilution of 1/100. Secondary Antibody: Red-fluorescent goat anti-mouse IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated ab96881 (2 different lots ) used at a dilution of 1/100 (5μg/mL). Counterstain: DAPI (blue)

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Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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