AB96881

Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed

Name: Goat Anti-Mouse IgG (DyLight® 594) preadsorbed secondary antibody (ab96881) | Abcam
Brand: Abcam Limited
SKU: AB96881

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(31 Publications)

Suitable for IHC-P, Flow Cyt, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 31 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed (AB96881)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed (AB96881)

ab96881 was used at dilution 1/100 for IHC analysis of FFPE human kidney. Primary Antibody : mouse anti-p53 (clone DO-1) used at a dilution of 1/100. Secondary Antibody : Red-fluorescent goat anti-mouse IgG-heavy and light chain cross-adsorbed Antibody DyLight^® 594 Conjugated ab96881 (2 different lots ) used at a dilution of 1/100 (5μg/mL). Counterstain : DAPI (blue)

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Rabbit, Goat, Horse, Chicken, Cow, Human, Pig

Pre-adsorbed

Yes

Conjugation

DyLight® 594

Excitation/Emission

Ex: 593nm, Em: 618nm

Applications

ICC/IF, Flow Cyt, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, pig, rabbit and rat IgG was detected.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (31)

Recent publications for all applications. Explore the full list and refine your search

Virology journal 22:189 PubMed40500754

2025

Lamin A/C promotes HBV transcription by modulating histone modification associated with cccDNA minichromosome in an HBx-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Linshan Jiang,Lanlang Peng,Hong Chen,Fangli Liao,Liping Yang,Yuanyuan Ye,Gaoli Zhang,Hua Sun,Qin Hu,Weixian Chen

Aging 16:7870-7888 PubMed38709288

2024

Leptin attenuates the osteogenic induction potential of BMP9 by increasing β-catenin malonylation modification via Sirt5 down-regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Kai-Xin Ke,Xiang Gao,Lu Liu,Wen-Ge He,Yue Jiang,Cheng-Bin Long,Gan Zhong,Zheng-Hao Xu,Zhong-Liang Deng,Bai-Cheng He,Ning Hu

Cell reports 43:113883 PubMed38430517

2024

Neural and metabolic dysregulation in PMM2-deficient human in vitro neural models.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Radenkovic,Rohit Budhraja,Teun Klein-Gunnewiek,Alexia Tyler King,Tarun N Bhatia,Anna N Ligezka,Karen Driesen,Rameen Shah,Bart Ghesquière,Akhilesh Pandey,Nael Nadif Kasri,Steven A Sloan,Eva Morava,Tamas Kozicz

Journal of cellular physiology 239:e31213 PubMed38308641

2024

UBC9-mediated SUMOylation of Lamin B1 enhances DNA-damage-induced nuclear DNA leakage and autophagy after spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-Peng Fan,Jun-Sheng Lou,Meng-Ran Jin,Cong-Hui Zhou,Hong-Hao Shen,Chun-Yan Fu,Xing-Jia Mao,Ying-Ying Chen,Jin-Jie Zhong,Lin-Lin Wang,Jun-Song Wu

Nature communications 14:8217 PubMed38086852

2023

Nuclear cGAS restricts L1 retrotransposition by promoting TRIM41-mediated ORF2p ubiquitination and degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengyi Zhen,Yu Chen,Haiyan Wang,Huanyin Tang,Haiping Zhang,Haipeng Liu,Ying Jiang,Zhiyong Mao

Frontiers in immunology 13:1089266 PubMed36713360

2023

is associated with tumor immunity and implies poor prognosis in glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Mingchen Yu,Yuanhao Chang,You Zhai,Bo Pang,Peng Wang,Guanzhang Li,Tao Jiang,Fan Zeng

Experimental lung research 49:12-26 PubMed36656657

2023

Hypoxic conditions promote a proliferative, poorly differentiated phenotype in COPD lung tissue progenitor cells in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Tina P Dale,Michael D Santer,Mohammed Haris,Wei Zuo,Nicholas R Forsyth

Experimental and therapeutic medicine 25:21 PubMed38895650

2022

miR‑146a‑5p protects against renal injury in MRL/lpr mice via improvement of the Treg/Th17 imbalance by targeting the TRAF6/NF‑κB axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajia Teng,Feng Yang,Xiaoling Li

The Journal of biological chemistry 298:102585 PubMed36223838

2022

Tick-borne encephalitis virus capsid protein induces translational shutoff as revealed by its structural-biological analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Selinger,Radim Novotný,Jakub Sýs,Justin A Roby,Hana Tykalová,Ganji Sri Ranjani,Marie Vancová,Kateřina Jaklová,Filip Kaufman,Marshall E Bloom,Zbyněk Zdráhal,Libor Grubhoffer,Jade K Forwood,Richard Hrabal,Michaela Rumlová,Ján Štěrba

Computational and structural biotechnology journal 20:2759-2777 PubMed35685361

2022

Integrative RNA profiling of TBEV-infected neurons and astrocytes reveals potential pathogenic effectors.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Selinger,Pavlína Věchtová,Hana Tykalová,Petra Ošlejšková,Michaela Rumlová,Ján Štěrba,Libor Grubhoffer

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed (AB96881)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Alternative Products

Secondary Antibodies

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Secondary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Additional targets

Publications (31)

Product promise