AB96874

Goat Anti-Mouse IgG H&L (DyLight® 650)

Name: Goat Anti Mouse (IgG) secondary antibody DyLight® 650 (ab96874) | Abcam
Brand: Abcam Limited
SKU: AB96874
Price: 265 USD
Availability: InStock

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(5 Publications)

Suitable for ICC/IF, Flow Cyt, IHC-P. Cited in 5 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

DyLight® 650

Excitation/Emission

Ex: 654nm, Em: 673nm

Applications

Flow Cyt, IHC-P, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments." } } }

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Product details

DyLight® 650 replaces DyLight® 649.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Animal reproduction science 211:106203 PubMed31785643

2019

Sperm cellular and nuclear dynamics associated with bull fertility.

Applications

Unspecified application

Species

Unspecified reactive species

Naseer A Kutchy,Erika S B Menezes,Muhammet R Ugur,Asma Ul Husna,Hazem ElDebaky,Holly C Evans,Emily Beaty,Fagner C Santos,Wei Tan,Robert W Wills,Einko Topper,Abdullah Kaya,Arlindo A Moura,Erdogan Memili

FASEB journal : official publication of the Federa 33:6551-6563 PubMed30794428

2019

C-reactive protein exacerbates epithelial-mesenchymal transition through Wnt/β-catenin and ERK signaling in streptozocin-induced diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Zhang,Zhi-Yuan Shen,Ke Wang,Wei Li,Jing-Ming Shi,Ezra Kombo Osoro,Naeem Ullah,Yan Zhou,Shang-Rong Ji

Cell stress & chaperones 22:589-600 PubMed28337642

2017

The small heat shock proteins αB-crystallin (HSPB5) and Hsp27 (HSPB1) inhibit the intracellular aggregation of α-synuclein.

Applications

Unspecified application

Species

Unspecified reactive species

Dezerae Cox,Heath Ecroyd

Journal of immunology (Baltimore, Md. : 1950) 194:5243-52 PubMed25917100

2015

C-reactive protein directly suppresses Th1 cell differentiation and alleviates experimental autoimmune encephalomyelitis.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Zhang,Shan-Hui Liu,Tyler T Wright,Zhi-Yuan Shen,Hai-Yun Li,Wei Zhu,Lawrence A Potempa,Shang-Rong Ji,Alexander J Szalai,Yi Wu

The Journal of biological chemistry 289:14283-90 PubMed24711458

2014

Topological localization of monomeric C-reactive protein determines proinflammatory endothelial cell responses.

Applications

Unspecified application

Species

Unspecified reactive species

Hai-Yun Li,Jing Wang,Yue-Xin Wu,Lin Zhang,Zu-Pei Liu,János G Filep,Lawrence A Potempa,Yi Wu,Shang-Rong Ji

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Goat Anti-Mouse IgG H&L (DyLight® 650)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Additional targets

Publications (5)

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